Izihlunu zeMagnetic Animal Tissue Genomic Kit

Ukubekwa ecaleni nokuhlanjululwa kwe-DNA ekumgangatho ophezulu kwizicwili zezilwanyana.

Ikhithi yamkela ubuhlalu bemagnethi ngomsebenzi owahlukileyo wokwahlula kunye nenkqubo ekhethekileyo ye-buffer yokwahlula nokucoca i-DNA ekumgangatho ophezulu kwiisampulu zezicubu zezilwanyana. Ubungangamsha obufihliweyo obahlukileyo bunobungqongqo obomeleleyo be-asidi ye-nucleic phantsi kweemeko ezithile. Xa iimeko zitshintsha, ubuhlalu bemagnethi buya kukhupha i-acidic acidic ads, ngaloo ndlela kufezekiswe injongo yokwahlukana okukhawulezayo kunye nokucocwa kwe-acid ye-nucleic. Yonke le nkqubo ikhuselekile kwaye kulula. Ngamaqhekeza amakhulu e-genomic e-genomic, ubunyulu obuphezulu kunye nomgangatho onokuthenjwa, le ndlela ilungele ngokukodwa ukufunyanwa okuzenzekelayo kwendawo yokusebenza.

Ikati. Hayi	Ubungakanani bokuPakisha
4992404	50 preps
4992405	200 preps
4992864	1000 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

FAQ

Iimpawu zemveliso

Iimbonakalo

■ Ilula kwaye iyakhawuleza: I-lysis ethe ngqo ngaphandle kokufukama, kunye ne-ultrapure genomic DNA inokufunyanwa kwisithuba seyure enye.
■ Ukuhamba okuphezulu: Ingadityaniswa nezixhobo ezizenzekelayo zendlela yokubetha kunye nendlela yentsimbi yemagnethi yovavanyo oluphezulu lokutsalwa kwemveliso.
■ Ukhuselekile kwaye awuyityhefu: Akukho zixhobo ze-organic ezinje nge phenol / chloroform ezifunekayo.
■ Ukucoceka okuphezulu: I-DNA efunyenweyo inobunyulu obuphezulu kwaye inokusetyenziswa ngokuthe ngqo ekufumaniseni i-chip, ukulandelelana okuphezulu kunye nolunye uvavanyo.

Izicelo

I-DNA esulungekileyo inokusetyenziswa kuvavanyo oluqhelekileyo oluqhelekileyo olufana nokwetyiswa kwe-enzyme, i-PCR, ukwakhiwa kwethala leencwadi, i-Southern hybridization, i-chip hybridization, ukulandelelana okuphezulu, njl.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-Serum / iPlasma ejikeleza i-Kit Kit

Okulandelayo: Amagqabantshintshi egazi Magnetic Kit

Sebenzisa iMagnetic Animal Tissue Genomic DNA Kit ukucoca i-DNA kwizicwili ezahlukeneyo zeempuku, intlanzi, i-shrimp, iqokobhe, iiseli ezinenkcubeko, inyama yomlenze wedada kunye nezinye iisampulu ngokwemiyalelo. M: Isiphawuli seTIANGEN D15000.
Umthamo we-Elution ngu-150 µl. I-1-2 µl eluent yayilayishwe kwi-agarose gel electrophoresis.
Iziphumo: I-TIAGNEN Magnetic Animal Tissue Genomic DNA Kit inoluhlu olubanzi lokusebenziseka kwesampulu, ngakumbi kwiisampulu ezinzima ezinje ngomsila wempuku, intlanzi, i-shrimp kunye ne-shellfish.

Sebenzisa i-TIAGNEN Magnetic Animal Tissue Genomic DNA Kit kunye neekiti ezifanelekileyo zoMthengisi M, O, A ukucoca i-DNA kwi-25 mg yeethishu zentliziyo ngokwemiyalelo. M: Isiphawuli seTIANGEN D15000.
Elution yevolumu: 150 .l. I-µl eluent ilayishwe kwi-agarose gel electrophoresis.
Iziphumo: I-TIAGNEN Magnetic Tissue Animal Genomic DNA Kit inesivuno esikhulu sokukhupha.

Umbuzo: Incinci okanye ayikho i-DNA kwi-eluent.

A-1 Ukuxinana kweeseli okanye intsholongwane kwisampulu yokuqala -Tyebisa uxinzelelo lweeseli okanye iintsholongwane.

A-2 I-lysis engonelanga yeesampulu -Iisampulu azange zixutywe kakuhle kunye ne-lysis buffer. Kucetyiswa ukuba udibanise ngokucofa i-vortexing kangangezihlandlo ezi-1-2. -I-lysis yeseli engonelanga ebangelwe kukuncipha kwemisebenzi ye-proteinase K. Kucetyiswa ukuba usike izicwili zibe ziingceba kwaye wandise ixesha lokuhlamba ukususa yonke intsalela yelysate.

A-3 Ukungoneliseki kwe-DNA adsorption. -Ayikho i-ethanol okanye ipesenti esezantsi endaweni ye-100% ye-ethanol eyongezwe ngaphambi kokuba kudluliselwe i-lysate kwikholamu yokujikeleza.

A-4 Ixabiso le-pH ye-elution buffer liphantsi kakhulu. Lungisa i-pH iye phakathi kwe-8.0-8.3.

Q: I-DNA ayisebenzi kakuhle kulingelo lokuphendula kwe-enzymatic reaction.

Intsalela ye-ethanol kwi-eluent.

-Kukho intsalela yokuhlambela i-PW kwisikali. I-ethanol ingasuswa ngokufaka phakathi kwikholamu ejikelezayo ye-3-5 min, kwaye emva koko ubeke kubushushu begumbi okanye kwi-50 ℃ incubator ye-1-2 min.

Q: Ukonakaliswa kweDNA

A-1 Isampulu ayisiyonto intsha. -Khupha isampulu yeDNA elungileyo njengokulawula ukumisela ukuba ingaba iDNA ekwisampulu ihlaziyiwe.

Unyango lwangaphambi kokunyanga okungalunganga. -Yenziwe kukugaya i-nitrogen engamanzi ngokugqithisileyo, ukubuyisa ukufuma, okanye isixa esikhulu sesampulu.

Umbuzo: Uyenza njani inyanzelo yokukhutshwa kwe-gDNA?

Ukufakwa kwangaphambili kufuneka kwahluka kwiisampulu ezahlukeneyo. Kwiisampulu zezityalo, qiniseka ukuba usile kakuhle kwi-nitrogen engamanzi. Kwiisampulu zezilwanyana, homogenate okanye ugaye kakuhle kwi-nitrogen engamanzi. Kwiisampulu ezineendonga zeseli ekunzima ukuziqhekeza, ezinjenge-G + bacteria kunye nemvubelo, kucetyiswa ukuba kusetyenziswe i-lysozyme, i-lyticase okanye iindlela zoomatshini zokwaphula iindonga zeseli.

Q: Nguwuphi umahluko phakathi kweekiti ezintathu zokukhupha i-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 ye-Genomic DNA Kit isebenzisa indlela esekwe kwikholamu efuna ikloroform yokukhupha. Ifanelekile ngakumbi kwiisampulu ezahlukeneyo zezityalo, kunye nomgubo owomileyo wezityalo. I-Hi-DNAsecure Plant Kit nayo isekwe kwikholamu, kodwa kungekho mfuneko yokukhutshwa kwe-phenol / chloroform, ukuyenza ikhuseleke kwaye ingabinetyhefu. Kufanelekile ukuba izityalo ezine-polysaccharides ephezulu kunye nomxholo we-polyphenol. 4992709/4992710 DNAquick Plant System yamkela indlela esekwe kulwelo. Ukukhutshwa kwePhenol / chloroform akufuneki nako. Inkqubo yokuhlanjululwa ilula kwaye iyakhawuleza ngaphandle komda kwiisampulu zezixa zokuqala, ke abasebenzisi banokuhlengahlengisa isixa ngokuhambelana neemfuno zovavanyo. Ubungakanani obukhulu bamaqhekeza e-gDNA anokufunyanwa ngesivuno esikhulu.

Sesiphi isivuno esiqikelelweyo se-gDNA kwi-1 ml yesampulu yegazi yi-TIANamp Blood DNA Kit?

I-genomic DNA yakhutshwa kwimilinganiselo eyahlukeneyo yeesampulu zabantu zegazi yi-TIANamp Blood DNA Kit. Iziphumo zilandelayo. Iziphumo zidweliswe njengereferensi kuphela, ezona ziphumo zokukhupha zixhomekeke kwimeko yeesampulu.

Q: Ngaba i-4992207/4992208 kunye ne-4992722/4992723 ingasetyenziselwa ukukhupha i-DNA yegazi?

Ukukhutshelwa kwegazi le-DNA kungena kusetyenziswa ii-reagents ezibonelelwe kwezi khithi zimbini ngokutshintsha nje umthetho olandelwayo kumyalelo othile wokukhutshwa kwegazi le-DNA. Ikopi ethambileyo yenkqubo yokutsalwa kwegazi kwi-DNA inokukhutshwa xa uceliwe.

Q: Xa ufaka isicelo se-TIANamp Genomic DNA Kit, ungaqhekeza njani izicwili ezitsha ekumisweni kweseli?

Misa isampulu entsha nge-1 ml ye-PBS, isaline eqhelekileyo okanye i-TE buffer. Ngokupheleleyo homogenize isampuli yi homogenizer kwaye uqokelele isitshisi ukuya ezantsi kumbhobho nge-centrifuging. Yilahle into engaphaya kwamandla, kwaye uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde wenziwe khona nge-200 μl buffer GA. Ukucocwa kwe-DNA elandelayo kunokwenziwa ngokomyalelo.

Q: Uyikhetha njani imveliso yokukhutshwa kwe-DNA kwiplasma, i-serum kunye neesampulu zamanzi omzimba?

Ukucoca i-gDNA kwi-plasma, i-serum kunye neesampuli zomzimba, kuyacetyiswa i-TIANamp Micro DNA Kit. Ukucoca intsholongwane gDNA kwiisampulu ze-serum / plasma, I-TIANamp Virus DNA / RNA Kit iyacetyiswa. Ukucoca i-gDNA yebhaktiriya kwiisampulu ze-serum kunye neplasma, i-TIANamp Bacteria DNA Kit iyacetyiswa (i-lysozyme kufuneka ibandakanywe kubhaktiriya). Kwiisampulu zamathe, iHi-Swab DNA Kit kunye ne-TIANamp Bacteria DNA Kit ziyacetyiswa.

Q: Ungazikhetha njani iikiti ze-gDNA zokukhupha kwiisampulu zomngundo?

I-DNAsecure Plant Kit okanye i-DNAquick Plant System iyacetyiswa ukuba isuswe kwi-fungal genome extraction. Ukukhutshwa kwegwele le-genome, i-TIANamp Yeast DNA Kit iyacetyiswa (i-lyticase kufuneka izilungiselele).

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