Magnetic Blood Genomic DNA Kit Featured Image

Ikhithi ye-Magnetic Genomic DNA Kit

Ukucoca okuphezulu kakhulu kwe-DNA ekumgangatho ophezulu ukusuka kwi-100 μl-1 ml yegazi.

Ikhithi yamkela ubuhlalu bemagnethi ngomsebenzi owahlukileyo wokwahlula kunye nenkqubo eyahlukileyo yesixhobo sokuzahlula nokucoca i-DNA ekumgangatho ophezulu kwi-100 μl-1 ml yegazi. Ubungangamsha obufihliweyo obahlukileyo bunobungqongqo obomeleleyo be-asidi ye-nucleic phantsi kweemeko ezithile. Xa iimeko zitshintsha, ubuhlalu bemagnethi bukhupha i-asidi ye-nucleic acid, ngaloo ndlela kufezekiswa injongo yokwahlukana okukhawulezayo kunye nokucocwa kwe-acid ye-nucleic. Yonke le nkqubo ikhuselekile kwaye kulula. Ngamaqhekeza amakhulu e-genomic e-genomic, ukusulungeka okuphezulu kunye nomgangatho onokuthenjwa, kufaneleke ngokukodwa ekutsalweni okuzenzekelayo kwendawo yokusebenza. I-DNA ehlanjululwe yikhithi inokulungela ukusebenza ngeendlela ezahlukeneyo, kubandakanya ukwetyiswa kwe-enzyme, i-PCR, ixesha lokwenyani le-PCR, ukwakhiwa kwethala leencwadi, ibloth esemaZantsi, ukufunyanwa kwe-chip, ukulandelelana okuphezulu kunye nolunye uvavanyo.

Ikati. Hayi	Ubungakanani bokuPakisha
4992402	50 preps
4992403	200 preps
4992976	1000 zangaphambili

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FAQ

Iimpawu zemveliso

Iimbonakalo

■ Ilula kwaye iyakhawuleza: I-Ultra-pure genomic DNA inokufumaneka kwisithuba seyure enye.
■ UkuPhuma okuphezulu: Inokudibanisa isixhobo esizenzekelayo sokubetha kunye nentonga yemagnethi ukwenza amalinge aphezulu okuphuma.
■ Ukhuselekile kwaye awuyityhefu: Akukho zixhobo zinetyhefu yezinto eziphilayo ezinjenge phenol / chloroform ezifunekayo.
■ Ukucoceka okuphezulu: I-DNA efunyenweyo inobunyulu obuphezulu kwaye inokusetyenziswa ngokuthe ngqo ekufumaniseni i-chip, ukulandelelana okuphezulu kunye nolunye uvavanyo.

Ukukhutshwa kwemveliso

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo Isitayile seTIANamp Stool DNA

Okulandelayo: I-TIANquick FFPE DNA Kit

Iziphumo zemilinganiselo yeDNA NanoDrop yeDNA ekhishwe kwigazi elingamakhulu amabini μl kusetyenziswa i-TIANGEN Magnetic Igazi le-Genomic DNA Kit kunye nemveliso efanelekileyo evela kuMthengisi G

Sebenzisa i-TIANGEN Magnetic Blood Genomic DNA Kit kunye nemveliso efanelekileyo kuMthengisi G ukukhupha i-DNA kwi-200 μl yegazi. I-DNA yadibana ne-200 μl Buffer TB. Thatha i-5 μl ye-electrophoresis, kunye ne-2 μl yeNanoDrop 2000 ukulinganisa uxinzelelo kunye nobunyulu. Iziphumo zibonisa ukuba imveliso yokutsalwa kweTIANGEN Magnetic Blood Genomic DNA Kit yi-18% ephezulu kunaleyo yoMthengisi uG.
M: Isiphawuli seTIANGEN D15000

Umbuzo: Incinci okanye ayikho i-DNA kwi-eluent.

A-1 Ukuxinana kweeseli okanye intsholongwane kwisampulu yokuqala -Tyebisa uxinzelelo lweeseli okanye iintsholongwane.

A-2 I-lysis engonelanga yeesampulu -Iisampulu azange zixutywe kakuhle kunye ne-lysis buffer. Kucetyiswa ukuba udibanise ngokucofa i-vortexing kangangezihlandlo ezi-1-2. -I-lysis yeseli engonelanga ebangelwe kukuncipha kwemisebenzi ye-proteinase K. Kucetyiswa ukuba usike izicwili zibe ziingceba kwaye wandise ixesha lokuhlamba ukususa yonke intsalela yelysate.

A-3 Ukungoneliseki kwe-DNA adsorption. -Ayikho i-ethanol okanye ipesenti esezantsi endaweni ye-100% ye-ethanol eyongezwe ngaphambi kokuba kudluliselwe i-lysate kwikholamu yokujikeleza.

A-4 Ixabiso le-pH ye-elution buffer liphantsi kakhulu. Lungisa i-pH iye phakathi kwe-8.0-8.3.

Q: I-DNA ayisebenzi kakuhle kulingelo lokuphendula kwe-enzymatic reaction.

Intsalela ye-ethanol kwi-eluent.

-Kukho intsalela yokuhlambela i-PW kwisikali. I-ethanol ingasuswa ngokufaka phakathi kwikholamu ejikelezayo ye-3-5 min, kwaye emva koko ubeke kubushushu begumbi okanye kwi-50 ℃ incubator ye-1-2 min.

Q: Ukonakaliswa kweDNA

A-1 Isampulu ayisiyonto intsha. -Khupha isampulu yeDNA elungileyo njengokulawula ukumisela ukuba ingaba iDNA ekwisampulu ihlaziyiwe.

Unyango lwangaphambi kokunyanga okungalunganga. -Yenziwe kukugaya i-nitrogen engamanzi ngokugqithisileyo, ukubuyisa ukufuma, okanye isixa esikhulu sesampulu.

Umbuzo: Uyenza njani inyanzelo yokukhutshwa kwe-gDNA?

Ukufakwa kwangaphambili kufuneka kwahluka kwiisampulu ezahlukeneyo. Kwiisampulu zezityalo, qiniseka ukuba usile kakuhle kwi-nitrogen engamanzi. Kwiisampulu zezilwanyana, homogenate okanye ugaye kakuhle kwi-nitrogen engamanzi. Kwiisampulu ezineendonga zeseli ekunzima ukuziqhekeza, ezinjenge-G + bacteria kunye nemvubelo, kucetyiswa ukuba kusetyenziswe i-lysozyme, i-lyticase okanye iindlela zoomatshini zokwaphula iindonga zeseli.

Q: Nguwuphi umahluko phakathi kweekiti ezintathu zokukhupha i-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 ye-Genomic DNA Kit isebenzisa indlela esekwe kwikholamu efuna ikloroform yokukhupha. Ifanelekile ngakumbi kwiisampulu ezahlukeneyo zezityalo, kunye nomgubo owomileyo wezityalo. I-Hi-DNAsecure Plant Kit nayo isekwe kwikholamu, kodwa kungekho mfuneko yokukhutshwa kwe-phenol / chloroform, ukuyenza ikhuseleke kwaye ingabinetyhefu. Kufanelekile ukuba izityalo ezine-polysaccharides ephezulu kunye nomxholo we-polyphenol. 4992709/4992710 DNAquick Plant System yamkela indlela esekwe kulwelo. Ukukhutshwa kwePhenol / chloroform akufuneki nako. Inkqubo yokuhlanjululwa ilula kwaye iyakhawuleza ngaphandle komda kwiisampulu zezixa zokuqala, ke abasebenzisi banokuhlengahlengisa isixa ngokuhambelana neemfuno zovavanyo. Ubungakanani obukhulu bamaqhekeza e-gDNA anokufunyanwa ngesivuno esikhulu.

Sesiphi isivuno esiqikelelweyo se-gDNA kwi-1 ml yesampulu yegazi yi-TIANamp Blood DNA Kit?

I-genomic DNA yakhutshwa kwimilinganiselo eyahlukeneyo yeesampulu zabantu zegazi yi-TIANamp Blood DNA Kit. Iziphumo zilandelayo. Iziphumo zidweliswe njengereferensi kuphela, ezona ziphumo zokukhupha zixhomekeke kwimeko yeesampulu.

Q: Ngaba i-4992207/4992208 kunye ne-4992722/4992723 ingasetyenziselwa ukukhupha i-DNA yegazi?

Ukukhutshelwa kwegazi le-DNA kungena kusetyenziswa ii-reagents ezibonelelwe kwezi khithi zimbini ngokutshintsha nje umthetho olandelwayo kumyalelo othile wokukhutshwa kwegazi le-DNA. Ikopi ethambileyo yenkqubo yokutsalwa kwegazi kwi-DNA inokukhutshwa xa uceliwe.

Q: Xa ufaka isicelo se-TIANamp Genomic DNA Kit, ungaqhekeza njani izicwili ezitsha ekumisweni kweseli?

Misa isampulu entsha nge-1 ml ye-PBS, isaline eqhelekileyo okanye i-TE buffer. Ngokupheleleyo homogenize isampuli yi homogenizer kwaye uqokelele isitshisi ukuya ezantsi kumbhobho nge-centrifuging. Yilahle into engaphaya kwamandla, kwaye uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde wenziwe khona nge-200 μl buffer GA. Ukucocwa kwe-DNA elandelayo kunokwenziwa ngokomyalelo.

Q: Uyikhetha njani imveliso yokukhutshwa kwe-DNA kwiplasma, i-serum kunye neesampulu zamanzi omzimba?

Ukucoca i-gDNA kwi-plasma, i-serum kunye neesampuli zomzimba, kuyacetyiswa i-TIANamp Micro DNA Kit. Ukucoca intsholongwane gDNA kwiisampulu ze-serum / plasma, I-TIANamp Virus DNA / RNA Kit iyacetyiswa. Ukucoca i-gDNA yebhaktiriya kwiisampulu ze-serum kunye neplasma, i-TIANamp Bacteria DNA Kit iyacetyiswa (i-lysozyme kufuneka ibandakanywe kubhaktiriya). Kwiisampulu zamathe, iHi-Swab DNA Kit kunye ne-TIANamp Bacteria DNA Kit ziyacetyiswa.

Q: Ungazikhetha njani iikiti ze-gDNA zokukhupha kwiisampulu zomngundo?

I-DNAsecure Plant Kit okanye i-DNAquick Plant System iyacetyiswa ukuba isuswe kwi-fungal genome extraction. Ukukhutshwa kwegwele le-genome, i-TIANamp Yeast DNA Kit iyacetyiswa (i-lyticase kufuneka izilungiselele).

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