Itekhnoloji yokuqonda ngemolekyuli, ngakumbi ukubonwa kwemetagenomic (mNGS), inezicelo ezifanelekileyo zokuchongwa kwesifo sepathogen, ukungaziwa kwesifo esitsha, isifo sokuchaphazeleka, ukuxilongwa kokuchasana neziyobisi, ukuvavanywa kokuphendula kwabantu kunye nokuvavanywa kwentsholongwane. Inika iindlela ezibalulekileyo zokuthintela kunye nokulawula izifo ezosulelayo, kwaye ikhuthaza ukuphuculwa kwesakhono sokuxilonga.
I-mNGS inokuhlalutya ngokubanzi i-microbial kunye ne-genetic material (i-DNA kunye ne-RNA) kwiisampulu zezigulana, kwaye ngokuthe ngcembe idluliselwe isuka kwilabhoratri iye kufakwa kwiklinikhi, njengenxalenye yesiseko sokuchongwa kokwahluka koogqirha, kunye nokubonelela ngenkxaso yokufumanisa isifo.
I-TIANGEN ibonelela ngesisombululo sokukhupha i-microorganism yoqobo kunye nolwakhiwo lwethala le-mNGS.
Ukuphendula kubhubhane we-COVID-19
Okoko i-COVID-19 breakout, i-TIANGEN ibonelele ngovavanyo lwezigidi ezi-5 njengezinto ezingafunekiyo zokukhupha intsholongwane ye-acid kunye ne-fluorescent yokufumanisa ubungakanani bezinto ezingaphezulu kwama-200 abavelisi bezenzi kunye neeyunithi zokufumanisa eAsia, eMantla nakuMzantsi Merika, kunye naseYurophu. Kwaye iyanceda izigidi zabantu kumazwe angaphezu kwama-30 kwihlabathi liphela.
Iimveliso zokukhutshwa kwentsholongwane ka-TIANGEN, njengezinto ezingavunyelwanga, zaqwalaselwa kwingxelo yovavanyo ngokusetyenziswa okungxamisekileyo kwe-COVID-19 ekhutshwe nguMbutho wezeMpilo weHlabathi ngoJuni 2020, zidweliswe kuluhlu olucetyiswayo lwee-reagents ezintsha ze-COVID-19 ezikhutshwe yi INgxowa-mali yeHlabathi ngoJanuwari 2021.
Ukusuka kwimpahla eluhlaza ukugqiba iimveliso
Zonke izixhumanisi zilawulwa ngokungqongqo phantsi kwenkqubo yolawulo lomgangatho we-ISO13485
Imveliso yokusiNgqongileyo
Iimpahla ezingekasilwa
Iimveliso ezingaphelelanga
I-QC-NGS-esekwe
Ukulondolozwa kweSampula
Isampulu yokuzibandakanya kwangaphambili
Ukukhutshwa kwe-acid yeNyukliya
NGS
Isampula yomlomo wesampula yoLondolozo
IRNAstore Reagent
Khusela i-RNA kwindawo engumkhenkce
Isicelo: ingqondo, intliziyo, izintso, udakada, isibindi, imiphunga kunye ne-thymus, njl.
Igcina i-RNA: usuku olu-1 kuma-37 ° C, iintsuku ezisi-7 nge-15-25 ° C, okanye iiveki ezi-4 nge-2-8 ° C. -20 ° C okanye -80 ° C
Isampuli yeethishu yokugaya
Isisombululo seHomogenization
Kukulungele ukukhutshwa kwe-DNA / RNA / protein ukusuka kwizicubu zezityalo / zezilwanyana, umhlaba, ilindle, ifungi, njl.
Ubushushu bokusebenza: njenge-10 ℃ iphantsi
Iziphumo: 1-24 iisampulu
Isiphumo sezilwanyana i-RNA ekhutshwe emva kokusila yi-H24R
Isixa sesampulu: 20 mg (intliziyo: 10mg) Isampulu: Umarker weCrucian carp: I-DNA MarkerIII, TIANGEN, MD103-02, RNA Kit Extraction: RNA Easy Fast Tissue / Cell Kit (TIANGEN, 4992732) I-RNA ekhutshwe emva kokusila yi-TGrinder H24R yoxinzelelo oluphezulu kunye nobunyulu.
Uthotho lwe-Nucleic Acid Extractor Series
● Amajelo angama-32 nama-96 ngokuzikhethela.
● Ukukhupha ngokukhawuleza intsholongwane ye-acid ngaphakathi kwemizuzu engama-30.
● Umgangatho ophezulu weekhithi ezizenzekelayo ezifumanekayo ukuze zisebenze ngokufanelekileyo.
Vula isisombululo sokuPhuma okuphezulu
IMagnetic Hi-DNA / RNA Kit (4992408 -T4A)
● Ukuhambelana okuphezulu, ukuhambelana ngokugqibeleleyo kunye nenkunkuma ye-nucleic acid kwimarike.
● Ukupakisha okwenziwe ngokwezifiso kunye neenkonzo ze-OEM ziyilelwe ngokweemfuno ezithile.
● Iyahambelana: KingFisher, Hamilton, Beckman Coulter, Chemagen njl.
Ixesha langempela le-PCR amplifi cation curve ye-TIANGEN 4992408
Ixesha langempela le-PCR amplifi cation curve yoMthengisi T
I-AIV-H5 yahlanjululwa yaya kwi-10-6 -10-8Iigradients ngamanzi e-Milli-Q, emva koko akhutshwa nguKing Fisher Flex. I-200 μl yesampulu nganye yasetyenziswa. Ikhithi ye-TIANGEN ibonise ubuntununtunu kunye nozinzo. Isixhobo se-PCR sokwenyani: I-ABI 7500 yeXesha langempela le-PCR yokufumanisa reagent: FP314
Isisombululo sokuSusa iManyuwali
● Kufuneka izixhobo ezilula kuphela ukugqibezela ulingelo lokutsala
● Izinto ezisetyenziswa zipakishwe zodwa ukunqanda ungcoliseko
● Ixesha elifutshane lokukhupha kunye nokusebenza okulula, ngokusebenza kakuhle.
● Ukupakisha okwenziwe ngokwezifiso kunye neenkonzo ze-OEM ziyilelwe ngokweemfuno ezithile.
I-TIANamp Virus DNA / RNA Kit (4992285)
I-TIANamp Virus RNA Kit (4992286)
I-TIANamp Micro DNA Kit (4992287)
| Ikati.no. | Uhlobo lwenyukliya | Uhlobo lwesampulu olusebenzayo |
| 4992285 | IDNA / iRNA | I-Serum, iplasma, ulwelo lomzimba, izicwili, isisombululo sokulondolozwa kwe-swab, intsholongwane phakathi kwenkcubeko, njl |
| 4992286 | RNA | I-Serum, iplasma, ulwelo lomzimba, izicwili, isisombululo sokulondolozwa kwe-swab, intsholongwane phakathi kwenkcubeko, njl |
| 4992287 | IDNA | Iibhaktheriya, iifungi, iiparasites kunye neentsholongwane ezizimeleyo kwi-serum, iplasma, izicubu, i-pleural kunye ne-ascites, i-cerebrospinal fluid, isikhohlela, i-bronchoalveolar lavage fluid kunye neparafini |
● I-TIANSeq i-DirectFast yeThala leeNcwadi (i-illumina) (4992259/4992260)
Ukuqhekeka kwe-Enzymatic DNA. Ilungele ukwakhiwa kwethala leencwadi le-DNA yeqonga lokulandelelana okuphezulu kwe-Illumina
● I-TIANSeq ekhawulezayo yeThala lencwadi le-DNA (illumina) (4992261/4992262)
Ilungele ukwakhiwa kwethala leencwadi le-DNA yeqonga lokulandelelana okuphezulu kwe-Illumina
● IKiti yeThala leNcwadi ekhawulezayo yeDNA yePlatform yeIon (izixhobo ezenziwe ngokwezifiso)
Ilungele ukwakhiwa kwethala leencwadi le-DNA ye-Ion yeqonga eliphezulu lokulandelela iqonga
● IKiti yeThala leNcwadi ekhawulezayo yeDNA yeQonga le-MGI (izixhobo ezenziwe ngokwezifiso)
Ilungele ukwakhiwa kwethala leencwadi le-DNA kwi-MGI yokulandelela okuphezulu kweqonga
● I-TIANSeq rRNA Depletion Kit (H / M / R) (4992363/4992364/4992391) (ye-Illumina / Ion torrent / yeqonga le-MGI)
Ukususwa komamkeli weRRNA ukuze kuphuculwe ubuntununtunu bokubonwa kweRNA yentsholongwane
● I-TIANSeq ekhawulezayo ye-RNA yeLayibrari yokuLungiselela iKhithi (i-Illumina) (4992375)
Ekwakhiweni kwethala leencwadi le-RNA yentsholongwane emva kokususa i-rRNA yokubamba, enokuchonga ngokukhawuleza ubukho bentsholongwane ye-RNA
● I-TIANSeq Stranded RNA-Seq Kit (Illumina) (4993007)
Ekwakhiweni kwethala leencwadi le-RNA yentsholongwane emva kokususa i-rRNA yomamkeli, enokuchonga ngokuchanekileyo umahluko wokulandelelana kwentsholongwane
● I-RNA RNA yeLayibrari yokuLungiselela iThala leQonga le-MGI (izixhobo ezenziwe ngokwezifiso)
Ilungele ukwakhiwa kwethala leencwadi le-RNA kwi-MGI yokuhamba ngokulandelelana okuphezulu kweqonga
● I-TIANSeq yoKhetho loKhetho lweDNA (4992358/4992359/4992979)
Ukucoca kunye nokukhetha ubungakanani beziqwenga zeDNA ngexesha lokwakhiwa kwethala leencwadi le-DNA
● I-TIANSeq RNA Amaso acocekileyo (4992360/4992362/4992867)
Ukucocwa kweRNA emva kokucetyiswa kweRNA
● I-TIANSeq Adapter-Index Adapter (Illumina) (4992642/4992378)
● I-TIANSeq ye-Dual-Index Adapter (i-Illumina) (NG216-T1 / 2/3/4/5/6)
Ukwakhiwa kwethala leencwadi le-DNA yeqonga lokulandelela okuphezulu kwe-Illumina
