NgoDisemba ngo-2019, uthotho lwamatyala enyumoniya esizathu esingaziwayo aqale eWuhan, kwiPhondo laseHubei, nase Kungekudala isasazeke kumaphondo nakwizixeko zase China, nakwamanye amazwe amaninzi ngoJanuwari 2020. Ukusukela nge-22: 00 ngokuhlwa Nge-27 kaJanuwari, i-28 iqinisekisile amatyala kunye nama-5794 akrokrelwa amatyala e-2019-nCov axeliwe e-China. Inkqubo ye-usuleleko lwentsholongwane lwathathwa njengeRhinolophus, kwaye inqanaba lokusweleka ngoku li-2.9%.
NgoJanuwari 12, 2020, uMbutho wezeMpilo weHlabathi (i-WHO) wabhengeza ukuba ubhubhane wenyumoniya wabangelwa luhlobo olutsha lwe-coronavirus (2019-nCov). ICoronavirus luhlobo lwentsholongwane eyasasazeka phakathi kwezilwanyana. Uhlobo lwentsholongwane ye-acid ye-acid luhlobo olunye lwe-RNA. Kwangelo xesha, i-WHO ikhuphe ulwazi ngokulandelelana kwe-acid ye-nCov ekwabelwana ngayo ngabaphengululi baseTshayina, ebonelele ngesiseko sokufunyanwa kwemolekyuli yokufumanisa intsholongwane kwaye yenze ukuba kubonwe ngokukhawuleza kunye nokuchongwa kwezifo.
I-WHO icebisa ukuba abantu abanezifo zokuphefumla ezibi ezifana nokukhohlela, umkhuhlane, usulelo lokuphefumla kwaye baye eWuhan kwiintsuku ezili-14 okanye bavezwa ukuba banxibelelana nezinye izigulana kufuneka bavavanywe ngokupheleleyo. Ngomhla we-17 kuJanuwari 2020, iWHO ikhuphe “uvavanyo lwelabhoratri lwenoveli ye-coronavirus ka-2019 (2019-nCoV) kumatyala abantu abarhanelwa, isikhokelo sethutyana, nge-17 kuJanuwari 2020”. Isikhokelo sichaza ukuba iisampulu eziza kuqokelelwa kwizigulana ezinophawu zibandakanya iisampulu zokuphefumla (i-nasopharynx kunye ne-oropharyngeal swabs, isikhohlela, i-bronchoalveolar lavage, njl.) Kunye neesampuli ze-serum, ngolu hlobo lulandelayo:
* Ukuhambisa iisampulu zokufumana intsholongwane, sebenzisa i-VTM (izixhobo zothutho lwentsholongwane) eziqulathe izithinteli zokubola kunye nezibulala ntsholongwane. Kwinkcubeko yebhaktiriya okanye yokungunda, ukuhambisa komile okanye kwisixa esincinci samanzi amdaka. Gwema ukupholisa okuphindaphindiweyo kunye nokunyibilika kweesampuli.
Ngaphandle kwezinto ezithile zokuqokelela eziboniswe kwitafile zikwaqinisekisa nezinye izinto kunye nezixhobo ezikhoyo: umz. Izikhongozeli zothutho kunye neengxowa zengqokelela yokuqokelelwa kunye nokupakishwa, izinto ezipholileyo kunye neepakethi ezibandayo okanye umkhenkce owomileyo, izixhobo zokutsala igazi ezingenazintsholongwane (umz. Iinaliti, iisirinji kunye neetyhubhu), iilebheli kunye nokumakisha okusisigxina, i-PPE, izixhobo zokucoca indawo.
Ukufumana intsholongwane, iisampulu ze-serum zinokusetyenziselwa ukukhangela intsholongwane ngendlela ye-immunological, ngelixa i-RTqPCR icetyiswa ukuba ibone i-nCov nucleic acid ukuze ikhawuleze ichaneke kwaye ichane ngakumbi. Njengentsholongwane
Ukulandelelana kuyaziwa, umxholo wentsholongwane ye-acid kwi-specimen inokufunyanwa ngokukhawuleza kuphela
ukukhetha ii-reagents ezifanelekileyo kunye neeprimers ezifanayo
(Ukuchongwa kwe-Wuhan coronavirus 2019 yi-real-time RTPCR, Protocol kunye novavanyo lokuqala ukusuka ngoJan 13, 2020) Njengenkokheli kwicandelo lokukhutshwa kwe-acid ye-acid kunye nokufumanisa e-China, I-TIANGEN BIOTECH (BEIJING) CO., LTD. ibonelele ngentsholongwane yonyawo lwenyawo kunye nomkhuhlane A (H1N1) ubhaqo lokufumana intsholongwane kubantu abangaphezulu kwesigidi se-10. Kwi-2019, intsholongwane ye-TIANGEN intsholongwane ye-acid ye-nucleic acid kunye nokufunyanwa kwezinto ezisetyenzisiweyo kuye kwasetyenziswa kwizigidi ezingama-30 zeesampulu zeengulube zaseAfrika, zenza igalelo elibonakalayo ekuchongeni nasekuthinteleni umkhuhlane weengulube zaseAfrika eChina. I-TIANGEN ayiniki nje ukukhutshwa kwentsholongwane ngokukhawuleza nangokuchanekileyo, kodwa ikwabonelela ngezixhobo zokugaya izicwili ezifanelekileyo kunye nezixhobo ze-nucleic acid ezizenzekelayo, ezenza iseti epheleleyo yezisombululo zokutsalwa kwentsholongwane kunye nokufunyanwa.
Isisombululo sokukhupha iAcidic Acid
I-TIANGEN Automated Nucleic Acid Extractors ngamaqonga azenzekelayo okukhutshwa kwe-acid ye-asidi kusetyenziswa indlela ye-magnetic bead. Ukusetyenziswa kwala maqonga akupheleli nje ekunciphiseni umthwalo wokuvavanywa kunye nokukhutshwa kwamasebe, kodwa kunciphisa neempazamo zokusebenza zokukhupha ngesandla, kwaye kuqinisekisa ukungaguquguquki kunye nozinzo lomgangatho we-nucleic acid.
I-TIANGEN I-automated Nucleic Acid Extractor ineendlela ezahlukeneyo (kubandakanya i-16, 24, 32, 48, 96 iitshaneli), kunye nezinto ezinokusetyenziswa ezinokusetyenziselwa ukukhutshwa kwe-acid ye-asidi kwiintlobo ezahlukeneyo zesampulu. I-TIANGEN ikwabonelela ngeenkonzo ezizenzekelayo zophuhliso kunye nokudityaniswa kweenkonzo ngokweemfuno ezahlukeneyo zovavanyo.
TGrinder H24 Izicubu Homogenizer
● Isetyenziselwa ngokubanzi ukugaya kunye nokwenza homogenizing izicwili kunye nelindle
● Ngokugaya homogenization amandla amaxesha 2-5 ukuba izixhobo zemveli
● Ukusila ngokungafaniyo kunye ne-homogenization, kuthintela ungcoliseko lomnqamlezo
● Isixhobo sokukhusela ngokuzenzekelayo ukukhusela abasebenzi baselebhu
I-TGuide S32 yeZithuthi zeNyukliya ezenzekelayo
● Ukuhamba kwesampulu: 1-32 iisampulu
● Ukucwangcisa umthamo: 20-1000 μl
● Uhlobo lwesampulu: Igazi, iiseli, izicwili, ilindle, intsholongwane kunye nezinye iisampulu
● Ixesha lokuqhubekeka: Ukuya kuthi ga kwi-8 min ukufumana i-viral nucleic acid
● Imowudi yolawulo: Imowudi yolawulo ezimbini zeWindows Pad kunye neqhosha lesikrini
● Ukuphuhliswa kweqonga: Iqonga elivulekileyo, simahla ukulinganisa ii-reagents
● I-TGuide S32 Magnetic Viral DNA / RNA Kit (DP604)
● Ukusetyenziswa okubanzi: Intsholongwane ye-DNA / i-RNA ekumgangatho ophezulu inokucocwa kwi-serum, iplasma, isampulu yeswabi, isisombululo sonyango lwethishu kunye nezisombululo ezahlukeneyo zokulondolozwa kwentsholongwane.
● Ilula kwaye iyasebenza: Le mveliso ihambelana ngokugqibeleleyo ne-TGuide S32 Automated Nucleic Acid Extractor, enokukhupha intsholongwane ye-DNA / i-RNA ngesivuno esikhulu, ubunyulu obuphezulu, umgangatho ozinzileyo nothembekileyo.
● Ukusetyenziswa kwezantsi: I-asidi ye-nucleic esulungekileyo ilungele ulingelo olusemazantsi lwentsholongwane ye-PCR kunye nexesha langempela le-PCR.
Isisombululo seNyukliya yokuSusa isisombululo
Njengenkokheli kwicandelo lokukhutshwa kwe-acid ye-acid kunye nokuchongwa, i-TIANGEN inoluhlu olubanzi kakhulu lweemveliso ze-acidic e-China, ezilungele ukubonwa kwe-coronavirus kuzo zonke iintlobo zeesampulu: igazi, i-serum / iplasma, izicwili, i-swab, intsholongwane njl.
I-TIANamp Virus DNA / RNA Kit (DP315)
● Ukusebenza okuphezulu: Umgangatho ophezulu wentsholongwane ye-DNA kunye ne-RNA inokufumaneka ngokucoca ngokukhawuleza nokuphindaphinda okuphezulu.
● Ukucoceka okuphezulu: Ukususwa ngokupheleleyo kwezingcolisi kunye nezithintelo kwisicelo esisezantsi.
● Ukhuseleko oluphezulu.
Isisombululo sokufumanisa intsholongwane yeRNA
1. Inkqubo yokuChwetheza ngokuZenzekelayo
● Ukuchaneka okuphezulu: Ibhloko lokupholisa lingagcina ubushushu besampulu ye-reagent engezantsi kwe-7 ℃ ngaphezulu kwe-60 min. I-APM nganye ilinganiswe ngokwemigangatho yamazwe aphesheya, enokuchaneka okuphezulu kunombhobho wokubhalwa ngesandla.
● Ukusebenza ngokulula: Ubungakanani obukhulu. Ubunzima bokukhanya. Akukho sixhobo siyafuneka endaweni yebhlokhi. Inkqubo eyakhiweyo yePCR / qPCR yokulungiselela. Umsebenzi olula wesandla wokulungiswa kwesisombululo sePCR.
● Ukusetyenziswa okubanzi: Inokusetyenziselwa i-96/384-ipleyiti yombhobho, i-PCR, i-qPCR, ukufunyanwa kwemfuza kunye nolunye uvavanyo oluphezulu.
Ukukhawulezisa inyathelo elinye kwi-RT-qPCR Kit (Probe) (FP314)
I-FastKing One Step RT-qPCR Kit (Probe) (FP314) ephuhliswe yi-TIANGEN yindlela yokubuyela umva ekhutshelweyo yokubonisa ubungakanani bokubona okusekwe kwindlela yophando, eyilelwe ngokukodwa ukufunyanwa kwemfuza yokulandelela kwiisampulu ezahlukeneyo. I-KingRTase kwikhithi yindlela entsha eguqulweyo ye-molekyuli eguqulweyo, ene-RNA eyomeleleyo kunye nokuzinza kwe-thermal, kunye nokuphuculwa kokukhutshelwa kokubuyela umva kunye nokwandiswa kwesakhiwo seetemplate ze-RNA ezintsonkothileyo. Isiqalo esitsha eshushu seTaq DNA polymerase siyasetyenziswa ukunika impendulo ye-PCR ukusebenza okuphezulu kokukhulisa kunye nokucaciswa. Ukongeza, ikhithi yenza lula izinto ukuya kuthi ga ngokuxuba i-Taq kunye ne-MLV enzyme kumxube we-enzyme, kunye nokuxuba kwangaphambili i-ion buffer, i-dNTPs, i-PCR stabilizer kunye ne-enhancer kwi-MasterMix, ukuze amanyathelo okuxuba amacandelo amaninzi abe
yenziwe lula.
● Ukusebenza okuphezulu: I-reverse transcriptase kunye ne-DNA polymerase eqinisekisa ukuphendula okuphezulu
● Ukubuyela umva okuhle: Ipolymerase inokufunda ngetemplate yeRNA enomxholo ophezulu we-GC kunye nolwakhiwo lwesekondari oluntsonkothileyo
● Ukusetyenziswa okubanzi: Ukusebenza okuphezulu kwiitemplate zeRNA ezinokungcola kwiintlobo ezahlukeneyo
● Ubuntununtunu obuphezulu: Iitemplate eziphantsi njenge-1 ng zinokuchongwa ngokuchanekileyo, ngakumbi kwiitemplate ezinobuninzi obusezantsi
Umzekelo wokuchongwa kwentsholongwane ye-RNA
I-nucleic acid ye-H5 avian influenza yakhutshwa yi-TGuide S32 Magnetic Viral DNA / RNA Kit (DP604). I-FastKing One Step RT-qPCR Kit (Probe) (FP314) yayisetyenziselwa ukufumanisa i-RT-qPCR kusetyenziswa i-H5 avian influenza primers kunye ne-probes.
I-ABi7500Fast yayisetyenziselwa ukufumanisa i-RT-qPCR. Iziphumo ze-H5 avian influenza virus antigen (10-2, 10-3, 10-4, 10-5, 10-6and 10-7 dilution) from 200 μl samples show high virus nucleic acid extraction yield, that can meet the following iimfuno zokukhuphela umva, i-PCR, i-RT-PCR, i-PCR yokwenene, njlnjl. Ukujongwa okungafaniyo kweesampulu ze-viral nucleic acid kunokufunyanwa ngokuchanekileyo.
Ixesha Post: Apr-11-2021
