RelaxGene Blood DNA System (0.1-20ml) Featured Image

RelaxGene Inkqubo yeDNA yeGazi (0.1-20ml)

Ukukhutshwa kwe-genomic DNA kwi-0.1-20 ml yegazi elitsha neligciniweyo eligcinwe nge-anticoagulants.

Inkqubo ye-RelaxGene yegazi ye-DNA isebenzisa inkqubo ye-buffer eyahlukileyo yokukhupha i-genomic DNA kwi-0.1-20 ml yegazi elitsha kunye nee-anticoagulants ezahlukeneyo kunye neesampulu zegazi. Inkqubo ye-buffer ingasusa iiproteni, i-pigment, i-lipids kunye nokunye ukungcola okungathinteliyo ukuya kuthi ga kwinqanaba eliphezulu, kunye nokufumana iziqwenga ezinkulu ze-DNA ngesivuno esikhulu, ubunyulu obuphezulu kunye nozinzo olulungileyo. Ikhithi ayifuni ukusebenzisa izinyibilikisi ezinjenge phenol / chloroform. I-DNA esulungekileyo inokusetyenziswa kuvavanyo olwahlukeneyo olufana nokuthintela ukwetyisa, i-PCR, ulwakhiwo lwethala leencwadi, ibloth esemaZantsi (ukuhanjiswa okuphezulu kokulandelelana kwe-chip hybridization), njl.

Ikati. Hayi	Ubungakanani bokuPakisha
4992722	50 ml
4992723	200 ml

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

FAQ

Iimpawu zemveliso

Iimbonakalo

■ Isixa sokuqala seFlexible sokuqala: I-2-400 μg i-DNA esulungekileyo inokukhutshwa kwi-0.1-20 ml yeesampulu zegazi ezahlukeneyo.
■ Ikhuselekile kwaye ithembekile: Akukho kungcoliseka kwezinyibilikisi ezinjenge phenol / chloroform.

Izicelo

Ilungele usetyenziso olwahlukeneyo oluqhelekileyo, olufana nokuthintela ukwetyisa, iPCR, ukwakhiwa kwethala leencwadi, ibloth esemaZantsi (i-chip hybridization yokulandelelana okuphezulu), njl.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-TIANamp yegazi yeDNA Kit

Okulandelayo: I-Hi-DNAsecurity Kit

	Ukucocwa kwe-genomic DNA ukusuka kwi-300 μl, 5 ml kunye ne-10 ml yegazi lokulwa ne-anticoagulation nge-EDTA kusetyenziswa iNkqubo yeDela yeGene yeGazi. I-3 μl ye-200 μl, i-500 μl kunye ne-1 ml eluates ngokwahlukeneyo zilayishwe kumgaqo ngamnye. M: Isiphawuli seTIANGEN D15000. Iziphumo zovavanyo: I-0.1-20 ml yegazi inokukhishwa yiRelaxGene System yeDNA yeDNA enoluhlu olufanelekileyo.
	Thelekisa i-genomic DNA ekhutshwe kwi-300 μl ye-anticoagulation yabantu kunye ne-EDTA kusetyenziswa iRelaxGene yeGazi leNkqubo yeDNA kunye neemveliso ezifanayo kwabanye abathengisi. M: Isiphawuli seTIANGEN D15000. I-3 μl ye-200 μl eluates ilayishwe ngomgaqo ngamnye. Iziphumo zovavanyo: Thelekisa nabanye abathengisi, iRelaxGene yegazi leNkqubo yeDNA inokufumana isivuno esiphezulu, ubunyulu kunye nokudityaniswa kwegazi le-gDNA.
	I-Genomic DNA yathatyathwa kwiisampulu zegazi kusetyenziswa iRelaxGene Blood DNA System. Iisampulu: I-300 μl yegazi lokulwa umntu nge-EDTA, i-EDTA / NaF, i-heparin kunye ne-sodium citrate; ibhatyi e-2 ml yegazi; I-10 μl yegazi yenkukhu kunye ne-200 μl yegazi legazi. I-3 μl ye-200 μl eluates ilayishwe ngomgaqo ngamnye. M: Isiphawuli seTIANGEN D15000. Iziphumo zovavanyo: RelaxGene Blood DNA System inokusetyenziswa ngokubanzi kumgangatho ophezulu wokucocwa kwe-DNA ye-anticoagulants yabantu ngonyango olwahlukeneyo kunye negazi leentlobo ezahlukeneyo.
	Olunye uQokelelo lweDatha

Umbuzo: Incinci okanye ayikho i-DNA kwi-eluent.

A-1 Ukuxinana kweeseli okanye intsholongwane kwisampulu yokuqala -Tyebisa uxinzelelo lweeseli okanye iintsholongwane.

A-2 I-lysis engonelanga yeesampulu -Iisampulu azange zixutywe kakuhle kunye ne-lysis buffer. Kucetyiswa ukuba udibanise ngokucofa i-vortexing kangangezihlandlo ezi-1-2. -I-lysis yeseli engonelanga ebangelwe kukuncipha kwemisebenzi ye-proteinase K. Kucetyiswa ukuba usike izicwili zibe ziingceba kwaye wandise ixesha lokuhlamba ukususa yonke intsalela yelysate.

A-3 Ukungoneliseki kwe-DNA adsorption. -Ayikho i-ethanol okanye ipesenti esezantsi endaweni ye-100% ye-ethanol eyongezwe ngaphambi kokuba kudluliselwe i-lysate kwikholamu yokujikeleza.

A-4 Ixabiso le-pH ye-elution buffer liphantsi kakhulu. Lungisa i-pH iye phakathi kwe-8.0-8.3.

Q: I-DNA ayisebenzi kakuhle kulingelo lokuphendula kwe-enzymatic reaction.

Intsalela ye-ethanol kwi-eluent.

-Kukho intsalela yokuhlambela i-PW kwisikali. I-ethanol ingasuswa ngokufaka phakathi kwikholamu ejikelezayo ye-3-5 min, kwaye emva koko ubeke kubushushu begumbi okanye kwi-50 ℃ incubator ye-1-2 min.

Q: Ukonakaliswa kweDNA

A-1 Isampulu ayisiyonto intsha. -Khupha isampulu yeDNA elungileyo njengokulawula ukumisela ukuba ingaba iDNA ekwisampulu ihlaziyiwe.

Unyango lwangaphambi kokunyanga okungalunganga. -Yenziwe kukugaya i-nitrogen engamanzi ngokugqithisileyo, ukubuyisa ukufuma, okanye isixa esikhulu sesampulu.

Umbuzo: Uyenza njani inyanzelo yokukhutshwa kwe-gDNA?

Ukufakwa kwangaphambili kufuneka kwahluka kwiisampulu ezahlukeneyo. Kwiisampulu zezityalo, qiniseka ukuba usile kakuhle kwi-nitrogen engamanzi. Kwiisampulu zezilwanyana, homogenate okanye ugaye kakuhle kwi-nitrogen engamanzi. Kwiisampulu ezineendonga zeseli ekunzima ukuziqhekeza, ezinjenge-G + bacteria kunye nemvubelo, kucetyiswa ukuba kusetyenziswe i-lysozyme, i-lyticase okanye iindlela zoomatshini zokwaphula iindonga zeseli.

Q: Nguwuphi umahluko phakathi kweekiti ezintathu zokukhupha i-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 ye-Genomic DNA Kit isebenzisa indlela esekwe kwikholamu efuna ikloroform yokukhupha. Ifanelekile ngakumbi kwiisampulu ezahlukeneyo zezityalo, kunye nomgubo owomileyo wezityalo. I-Hi-DNAsecure Plant Kit nayo isekwe kwikholamu, kodwa kungekho mfuneko yokukhutshwa kwe-phenol / chloroform, ukuyenza ikhuseleke kwaye ingabinetyhefu. Kufanelekile ukuba izityalo ezine-polysaccharides ephezulu kunye nomxholo we-polyphenol. 4992709/4992710 DNAquick Plant System yamkela indlela esekwe kulwelo. Ukukhutshwa kwePhenol / chloroform akufuneki nako. Inkqubo yokuhlanjululwa ilula kwaye iyakhawuleza ngaphandle komda kwiisampulu zezixa zokuqala, ke abasebenzisi banokuhlengahlengisa isixa ngokuhambelana neemfuno zovavanyo. Ubungakanani obukhulu bamaqhekeza e-gDNA anokufunyanwa ngesivuno esikhulu.

Sesiphi isivuno esiqikelelweyo se-gDNA kwi-1 ml yesampulu yegazi yi-TIANamp Blood DNA Kit?

I-genomic DNA yakhutshwa kwimilinganiselo eyahlukeneyo yeesampulu zabantu zegazi yi-TIANamp Blood DNA Kit. Iziphumo zilandelayo. Iziphumo zidweliswe njengereferensi kuphela, ezona ziphumo zokukhupha zixhomekeke kwimeko yeesampulu.

Q: Ngaba i-4992207/4992208 kunye ne-4992722/4992723 ingasetyenziselwa ukukhupha i-DNA yegazi?

Ukukhutshelwa kwegazi le-DNA kungena kusetyenziswa ii-reagents ezibonelelwe kwezi khithi zimbini ngokutshintsha nje umthetho olandelwayo kumyalelo othile wokukhutshwa kwegazi le-DNA. Ikopi ethambileyo yenkqubo yokutsalwa kwegazi kwi-DNA inokukhutshwa xa uceliwe.

Q: Xa ufaka isicelo se-TIANamp Genomic DNA Kit, ungaqhekeza njani izicwili ezitsha ekumisweni kweseli?

Misa isampulu entsha nge-1 ml ye-PBS, isaline eqhelekileyo okanye i-TE buffer. Ngokupheleleyo homogenize isampuli yi homogenizer kwaye uqokelele isitshisi ukuya ezantsi kumbhobho nge-centrifuging. Yilahle into engaphaya kwamandla, kwaye uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde wenziwe khona nge-200 μl buffer GA. Ukucocwa kwe-DNA elandelayo kunokwenziwa ngokomyalelo.

Q: Uyikhetha njani imveliso yokukhutshwa kwe-DNA kwiplasma, i-serum kunye neesampulu zamanzi omzimba?

Ukucoca i-gDNA kwi-plasma, i-serum kunye neesampuli zomzimba, kuyacetyiswa i-TIANamp Micro DNA Kit. Ukucoca intsholongwane gDNA kwiisampulu ze-serum / plasma, I-TIANamp Virus DNA / RNA Kit iyacetyiswa. Ukucoca i-gDNA yebhaktiriya kwiisampulu ze-serum kunye neplasma, i-TIANamp Bacteria DNA Kit iyacetyiswa (i-lysozyme kufuneka ibandakanywe kubhaktiriya). Kwiisampulu zamathe, iHi-Swab DNA Kit kunye ne-TIANamp Bacteria DNA Kit ziyacetyiswa.

Q: Ungazikhetha njani iikiti ze-gDNA zokukhupha kwiisampulu zomngundo?

I-DNAsecure Plant Kit okanye i-DNAquick Plant System iyacetyiswa ukuba isuswe kwi-fungal genome extraction. Ukukhutshwa kwegwele le-genome, i-TIANamp Yeast DNA Kit iyacetyiswa (i-lyticase kufuneka izilungiselele).

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