RNA Easy Fast Plant Tissue Kit Featured Image

I-RNA Kit ekhawulezayo yeTissue Kit

Ukucoca i-RNA ekumgangatho opheleleyo kwizicubu zezityalo.

I-RNA Easy Plant Tissue Kit sisixhobo esikhawulezayo sokukhupha i-RNA yezihlunu zezityalo eziphuhliswe ngokusekwe kwitekhnoloji ye-genomic yokususa itekhnoloji eyenziwe nguTIANGEN kuphela. Izenzi ezinetyhefu ezinje nge-β-mercaptoethanol okanye i-DTT azifuneki ngexesha lokusebenza, kwaye yonke inkqubo yokukhutshwa kweRNA inokugqitywa kungaphelanga imizuzu engama-30. Ayilungelanga kuphela iisampulu zamagqabi ezityalo eziqhelekileyo ezinje ngengqolowa nombona, kodwa nakwiisampulu ezityebileyo zepolysaccharide / polyphenol ezinje ngegqabi leMalus spectabilis, igqabi lomqhaphu, igqabi le-chrysanthemum, intyatyambo ye-hibiscus, i-potato tuber, ivatala, ikhukhamba, inaliti yepayini njl.

Ikati. Hayi	Ubungakanani bokuPakisha
4992880	50 preps

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FAQ

Iimpawu zemveliso

Iimbonakalo

■ ilula kwaye iyakhawuleza: Iyonke iRNA yokukhupha iisampulu zezityalo inokugqitywa kwisithuba semizuzu engama-30.
■ Ukuhambelana okunamandla: Le khithi ayilungelanga kuphela iisampuli zesiqu kunye namagqabi, kodwa kunye neesampulu ze-polysaccharide / polyphenol.
■ Ikhuselekile kwaye iphantsi inetyhefu: Izinto ezinetyhefu ezinje nge-β-mercaptoethanol, DTT, chloroform, phenol azifuneki.

Izicelo

■ Ilungele ukusebenza ngasemazantsi, kubandakanya i-RT-PCR, i-RT-qPCR, i-chip hybridization, i-Northern Blot, iDot Blot, ukuvavanywa kwe-PolyA, inguqulelo ye-vitro, uhlalutyo lokhuselo lwe-RNase kunye nokwenziwa kwemolekyuli, njl.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-RNA Tissue ekhawulezayo / iselfowuni

Okulandelayo: I-TGuide S32 Magnetic Kit Kit yegazi

	I-RNA iyonke yeesampulu ezingamagqabi ezingama-65 mg (amagqabi e-clove, amagqabi e-hibiscus, amagqabi engqolowa, amagqabi erayisi, amagqabi eziqhamo ezi-ugli, amagqabi ePrunus cerasifera, amagqabi omkhiwane, amagqabi emini, amagqabi e-Kerria japonica), iisampulu zefungus eziyi-150 mg (i-Lentinus edode) kunye ne-200 Iisampulu ze-mg petal (i-pet-lily petals) zakhutshwa zisebenzisa i-RNA Easy Plant Tissue Kit.
	I-Agilent Bioanalyzer 2100 isiphumo sohlalutyo lwe-RNA iyonke kwiipalali zeSuku lweenyibiba.
	I-RNA ikhutshwe kwiisampulu ezahlukeneyo ezidweliswe kwitafile kusetyenziswa iRNA Easy Plant Tissue Kit. Ubungakanani bevolumu: 50 μl; Ukulayisha ivolumu: 2 μl. I-electrophoresis yenziwa nge-6 V / cm kwimizuzu eli-15 kwi-1% ye-agarose.

Q: Ikholamu yokuvalela

I-A-1 Cell lysis okanye i-homogenization ayonelanga

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampuli esetyenzisiweyo okanye ukwandisa inani le-lysis buffer.

Q: Isivuno seRNA esisezantsi

A-1 I-lysis yeseli engonelanga okanye i-homogenization

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

- Nceda ubhekise kubungakanani bokusebenza.

I-A-3 RNA ayikhutshiwe ngokupheleleyo kwikholamu

--Ngasemva kokongeza amanzi asimahla e-RNase, yishiye imizuzu embalwa ngaphambi kokufaka isenti.

I-A-4 Ethanol elufeni

- Emva kokucoca, icentrifuge kwakhona kwaye ususe isikhuseli sokuhlamba kangangoko.

I-A-5 yesiko lenkcubeko yeseli ayisuswanga ngokupheleleyo

- Xa uqokelela iiseli, nceda uqiniseke ukuba ususa indawo yenkcubeko kangangoko kunokwenzeka.

A-6 Iiseli ezigcinwe kwiRNAstore azisebenzi kakuhle kwisenti

--Uxinano lweRNAstore lukhulu kunophakathi ophakathi kwenkcubeko yeseli; ke amandla e-centrifugal kufuneka anyuswe. Kuyacetyiswa kwi-centrifuge kwi-3000x g.

Umxholo we-A-7 Low RNA kunye nobuninzi kwisampulu

--Sebenzisa isampulu elungileyo ukumisela ukuba ngaba isivuno esisezantsi sibangelwa sisampulu.

Q: Ukuthotywa kweRNA

A-1 Imathiriyeli ayisiyonto intsha

Izicubu ezintsha kufuneka zigcinwe kwi-nitrojeni engamanzi kwangoko okanye kwangoko ibekwe kwi-RNAstore reagent ukuqinisekisa ukusebenza kokukhutshwa.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-3 RNase ukungcolan

- Nangona i-buffer ebonelelwe kwikhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngexesha lenkqubo yokukhupha kwaye kufuneka iphathwe ngononophelo.

Ungcoliseko lwe-4-Electrophoresis

---- Faka endaweni ye-electrophoresis buffer kwaye uqiniseke ukuba izinto ezisetyenziswayo kunye nokuLayisha iBuffer zikhululekile kungcoliseko lweRNase.

A-5 Ukulayisha kakhulu i-electrophoresis

---- Ukunciphisa inani lokulayisha isampula, ukulayishwa kwequla ngalinye akufuneki kudlule kwi-2 μg.

Umbuzo: Ungcoliseko lwe-DNA

A-1 Isixa sesampulu sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-2 Ezinye iisampulu zinomxholo ophezulu we-DNA kwaye zinokunyangwa nge-DNase.

- Yenza unyango lwe-RNase-Simahla ye-DNase kwisisombululo esifunyenweyo se-RNA, kwaye i-RNA inokusetyenziselwa ngokuthe ngqo kuvavanyo olulandelayo emva konyango, okanye inokucocwa ngakumbi ziikhithi zokucocwa zeRNA.

Q: Uyisusa njani i-RNase kwizinto ezisetyenziswayo zovavanyo nakwiglasi?

Ngeiglasi, kubhakwe kwi-150 ° C nge-4 h. Kwizikhongozeli zeplastiki, zibhaptizwe kwi-0.5 M NaOH kangange-10 min, emva koko zahlanjwa kakuhle ngamanzi angenasimahla e-RNase kwaye emva koko zibulale ukuze ususe i-RNase ngokupheleleyo. Izinto ezisetyenziswayo okanye isisombululo esisetyenzisiweyo kulingo, ngakumbi amanzi, kufuneka simahla kwi-RNase. Sebenzisa amanzi asimahla e-RNase kuwo onke amalungiselelo e-reagent (yongeza amanzi kwibhotile yeglasi ecocekileyo, yongeza i-DEPC kuxinzelelo lokugqibela lwe-0.1% (V / V), gungqisa ubusuku kunye ne-autoclave).

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