RNA Easy Fast Tissue/Cell Kit Featured Image

I-RNA Tissue ekhawulezayo / iselfowuni

Ukucocwa komgangatho ophezulu we-RNA epheleleyo kwizicwili / iiseli zezilwanyana.

I-RNA Easy Tissue / Cell Kit yinto ekhawulezayo yokukhupha iRNA yezihlunu zezilwanyana / iisampulu zeseli. Iphuhlisiwe ngokusekwe kwitekhnoloji ye-genomic yokususa itekhnoloji eyenziwe ngu-TIANGEN kuphela. Inani elikhulu leesampulu ezahlukeneyo zinokuqwalaselwa ngaxeshanye ngenkqubo yokukhawuleza ngaphakathi kwimizuzu engama-30. I-RNA ebekelwe bucala yile mveliso inokusebenza ngokuthe ngqo njengeetemplate zokufumana ukuhla okanye ezinye iinkqubo.

Ikati. Hayi	Ubungakanani bokuPakisha
4992732	50 preps

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FAQ

Iimpawu zemveliso

Iimbonakalo

■ Ukusebenza ngokukhawuleza: I-RNA inokufunyanwa kwisithuba semizuzu engama-30.
■ Ukucoceka okuphezulu: I-Silica membrane isusa konke ukungcola kwaye ikholamu yokususa i-gDNA isusa i-genomic DNA.
■ Ukusetyenziswa okubanzi: Zilungele iisampulu ezahlukeneyo ezinje ngezicubu, iiseli kunye neebhaktiriya.

Ukucaciswa

Uhlobo: Spin ikholamu esekwe
Isampulu kunye nokuqala kwevolumu: I-10-20 mg yethishu okanye <10⁷ iiseli
Ithagethi: RNA
Ixesha lokusebenza: ~ Imizuzu engama-30
Izicelo ezisezantsi: RT-PCR / RT-qPCR, Northern Blot, Dot Blot, poly (A) ukhetho, in vitro translation, RNase protection assay, molecular cloning, njl.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-RNYenza i-Hi-Blood Kit emsulwa

Okulandelayo: I-RNA Kit ekhawulezayo yeTissue Kit

I-RNA ikhutshwe kwisampulu yesibindi esine-15 mg yesampulu kusetyenziswa i-RNA Easy Tissue / Cell Kit kunye nemveliso efanelekileyo kumthengisi u-A kunye no-T.
Ubungakanani bevolumu: 100 μl; Ukulayisha ivolumu: 3 μl
M: Isiphawuli seTIANGEN D15000
Iziphumo zokulinga: I-RNA Easy Tissue / Cell Kit ineqondo lokutsala eliphezulu kunemveliso efanelekileyo evela kumthengisi uA kunye no-T.

Q: Ikholamu yokuvalela

I-A-1 Cell lysis okanye i-homogenization ayonelanga

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampuli esetyenzisiweyo okanye ukwandisa inani le-lysis buffer.

Q: Isivuno seRNA esisezantsi

A-1 I-lysis yeseli engonelanga okanye i-homogenization

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

- Nceda ubhekise kubungakanani bokusebenza.

I-A-3 RNA ayikhutshiwe ngokupheleleyo kwikholamu

--Ngasemva kokongeza amanzi asimahla e-RNase, yishiye imizuzu embalwa ngaphambi kokufaka isenti.

I-A-4 Ethanol elufeni

- Emva kokucoca, icentrifuge kwakhona kwaye ususe isikhuseli sokuhlamba kangangoko.

I-A-5 yesiko lenkcubeko yeseli ayisuswanga ngokupheleleyo

- Xa uqokelela iiseli, nceda uqiniseke ukuba ususa indawo yenkcubeko kangangoko kunokwenzeka.

A-6 Iiseli ezigcinwe kwiRNAstore azisebenzi kakuhle kwisenti

--Uxinano lweRNAstore lukhulu kunophakathi ophakathi kwenkcubeko yeseli; ke amandla e-centrifugal kufuneka anyuswe. Kuyacetyiswa kwi-centrifuge kwi-3000x g.

Umxholo we-A-7 Low RNA kunye nobuninzi kwisampulu

--Sebenzisa isampulu elungileyo ukumisela ukuba ngaba isivuno esisezantsi sibangelwa sisampulu.

Q: Ukuthotywa kweRNA

A-1 Imathiriyeli ayisiyonto intsha

Izicubu ezintsha kufuneka zigcinwe kwi-nitrojeni engamanzi kwangoko okanye kwangoko ibekwe kwi-RNAstore reagent ukuqinisekisa ukusebenza kokukhutshwa.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-3 RNase ukungcolan

- Nangona i-buffer ebonelelwe kwikhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngexesha lenkqubo yokukhupha kwaye kufuneka iphathwe ngononophelo.

Ungcoliseko lwe-4-Electrophoresis

---- Faka endaweni ye-electrophoresis buffer kwaye uqiniseke ukuba izinto ezisetyenziswayo kunye nokuLayisha iBuffer zikhululekile kungcoliseko lweRNase.

A-5 Ukulayisha kakhulu i-electrophoresis

---- Ukunciphisa inani lokulayisha isampula, ukulayishwa kwequla ngalinye akufuneki kudlule kwi-2 μg.

Umbuzo: Ungcoliseko lwe-DNA

A-1 Isixa sesampulu sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-2 Ezinye iisampulu zinomxholo ophezulu we-DNA kwaye zinokunyangwa nge-DNase.

- Yenza unyango lwe-RNase-Simahla ye-DNase kwisisombululo esifunyenweyo se-RNA, kwaye i-RNA inokusetyenziselwa ngokuthe ngqo kuvavanyo olulandelayo emva konyango, okanye inokucocwa ngakumbi ziikhithi zokucocwa zeRNA.

Q: Uyisusa njani i-RNase kwizinto ezisetyenziswayo zovavanyo nakwiglasi?

Ngeiglasi, kubhakwe kwi-150 ° C nge-4 h. Kwizikhongozeli zeplastiki, zibhaptizwe kwi-0.5 M NaOH kangange-10 min, emva koko zahlanjwa kakuhle ngamanzi angenasimahla e-RNase kwaye emva koko zibulale ukuze ususe i-RNase ngokupheleleyo. Izinto ezisetyenziswayo okanye isisombululo esisetyenzisiweyo kulingo, ngakumbi amanzi, kufuneka simahla kwi-RNase. Sebenzisa amanzi asimahla e-RNase kuwo onke amalungiselelo e-reagent (yongeza amanzi kwibhotile yeglasi ecocekileyo, yongeza i-DEPC kuxinzelelo lokugqibela lwe-0.1% (V / V), gungqisa ubusuku kunye ne-autoclave).

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