■ Ukhuseleko olufanelekileyo: Khusela i-RNA egazini elitsha ekonakaleni.
Con Lula: Olu tyando luthatha amanyathelo ama-2 kuphela kwaye lulungele ukugcinwa kwesampulu enkulu yegazi.
■ ukuhambelana ngokugqibeleleyo: Iyahambelana neTIANGEN's proprietary Silicon Matrix Membrane Ukucocwa Kits ukukhupha iRNA yegazi okanye iDNA ukuqinisekisa ukukhutshwa kwemveliso kunye nobunyulu.
Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)
Izinto eziphathekayo: 100 μl igazi elitsha lempuku Indlela: I-RNA iyonke yegazi lempuku elitsha (eligcinwe kwi-RNALock Reagent) eligcinwe kwi-15-25 ℃, 4-8 ℃, -20 / -70 ℃ zahlulwa zisebenzisa i-RNAprep Pure Blood Kit (4992238) elandela umgaqo we-RNALock . Iziphumo: Nceda ubone lo mfanekiso ungasentla we-agarose gel electrophoresis. I-4-6 μl ye-50 μl eluates ilayishwe ngomgaqo ngamnye. M: ICANDELO LE-DNA Phawu III; Umgaqo 1-2 (ulawulo olululo): I-RNA ihlanjululwe kwigazi elitsha; Umgaqo 3-4: I-RNA ihlanjululwe kwiisampulu zegazi ezigcinwe kwi-15-25 ℃ kangangeentsuku ezimbini. Umgaqo 5-6: I-RNA ihlanjululwe kwiisampulu zegazi ezigcinwe kwi-4-8 ℃ kwiveki enye. Umgaqo 7-8: I-RNA ihlanjululwe kwiisampulu zegazi ezigcinwe kwi -20 ℃ okanye -70 ℃ kangangesiqingatha sonyaka. I-electrophoresis yenziwa nge-6 V / cm kwimizuzu engama-30 kwi-1% ye-agarose. |
Oko yasekwa, umzi-mveliso wethu uphuhlisa iimveliso zodidi lokuqala lwehlabathi ngokubambelela kumgaqo
esemgangathweni kuqala. Iimveliso zethu ziye zaziwa ngokugqwesileyo kushishino kunye nexabiso lokuthenjwa phakathi kwabathengi abatsha nabadala ..