I-RNYenza i-Hi-Blood Kit emsulwa

Ukucocwa komgangatho ophezulu kunye nokuzinzileyo kweRNA iyonke egazini.

I-RNAprep i-Hi-Blood Kit emsulwa ngokuchanekileyo ikhupha i-RNA iyonke kwigazi kunye negazi elitsha kunye ne-anticoagulants ezininzi ezivela kwiindidi ezahlukeneyo. Izinto zeatrix zesilicon ezisetyenziswe kwikholamu ye-adsorption yinto entsha eyahlukileyo eyenziwe yi-TIANGEN, esebenza ngokufanelekileyo nangokuchanekileyo kwi-RNA, kwaye isuse iiproteni zokungahlambuluki ukuya kuthi ga kwinqanaba eliphezulu. I-RNA ekhutshiweyo inokusetyenziswa kuvavanyo olwahlulayo olwahlukeneyo njenge-RT-PCR, i-RT-qPCR, uhlalutyo lwe-chip, ukulandelelana okuphezulu, iNorthern Blot, iDot Blot, ukuvavanywa kwePolyA, ukuguqulelwa kwe-vitro, uhlalutyo lokhuselo lwe-RNase, ubumbano lweemolekyuli, njl.

Ikati. Hayi	Ubungakanani bokuPakisha
4992903	50 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

FAQ

Iimpawu zemveliso

Iimbonakalo

■ Ilungele lonke igazi elitsha elipheleleyo leentlobo ezahlukeneyo, ekulula ukusebenziseka.
■ Ukuxhotyiswa nge-RNase-Free Filtration Column CS ukususa ngokufanelekileyo ukungcola.
■ Isikhuseli esenziwe ngokukodwa sinokuqinisekisa ukukhutshwa okukuko kunye nokuzinzileyo kwe-RNA kwizintlu ezahlukeneyo zamalinge asezantsi.
■ Ukusebenza okukhuselekileyo nokuthembekileyo, akukho phenol / chloroform yokukhutshwa kufunekayo.

Izicelo

I-RT-PCR, i-Northern Blot, i-RT-qPCR, uhlalutyo lwe-chip, ukulandelelana okuphezulu, ukuvavanywa kwePolyA, uhlalutyo lokhuselo lwe-RNase, ukuguqulelwa kwe-vitro, njl.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo RNAprep Isityalo esicocekileyo kunye neKhithi

Okulandelayo: I-RNA Tissue ekhawulezayo / iselfowuni

	Umzobo 1. I-RNA ihlanjululwe kwi-100 μl yegazi elitsha le-rat kwi-anticoagulants ezahlukeneyo zisebenzisa i-RNAprep Pure Hi-Blood Kit. I-4-6 μl ye-50 μl eluates ilayishwe ngomgaqo ngamnye. M: ICandelo leDNA eliThengisiweyo le-DNA.
	Umzobo 2. I-RNA ihlanjululwe kwi-100 μl yegazi elitsha lempuku kusetyenziswa i-RNAprep Pure Hi-Blood Kit. I-4-6 μl ye-50 μl eluates ilayishwe ngomgaqo ngamnye. M: ICandelo leDNA eliThengisiweyo le-DNA.

Q: Ikholamu yokuvalela

I-A-1 Cell lysis okanye i-homogenization ayonelanga

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampuli esetyenzisiweyo okanye ukwandisa inani le-lysis buffer.

Q: Isivuno seRNA esisezantsi

A-1 I-lysis yeseli engonelanga okanye i-homogenization

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

- Nceda ubhekise kubungakanani bokusebenza.

I-A-3 RNA ayikhutshiwe ngokupheleleyo kwikholamu

--Ngasemva kokongeza amanzi asimahla e-RNase, yishiye imizuzu embalwa ngaphambi kokufaka isenti.

I-A-4 Ethanol elufeni

- Emva kokucoca, icentrifuge kwakhona kwaye ususe isikhuseli sokuhlamba kangangoko.

I-A-5 yesiko lenkcubeko yeseli ayisuswanga ngokupheleleyo

- Xa uqokelela iiseli, nceda uqiniseke ukuba ususa indawo yenkcubeko kangangoko kunokwenzeka.

A-6 Iiseli ezigcinwe kwiRNAstore azisebenzi kakuhle kwisenti

--Uxinano lweRNAstore lukhulu kunophakathi ophakathi kwenkcubeko yeseli; ke amandla e-centrifugal kufuneka anyuswe. Kuyacetyiswa kwi-centrifuge kwi-3000x g.

Umxholo we-A-7 Low RNA kunye nobuninzi kwisampulu

--Sebenzisa isampulu elungileyo ukumisela ukuba ngaba isivuno esisezantsi sibangelwa sisampulu.

Q: Ukuthotywa kweRNA

A-1 Imathiriyeli ayisiyonto intsha

Izicubu ezintsha kufuneka zigcinwe kwi-nitrojeni engamanzi kwangoko okanye kwangoko ibekwe kwi-RNAstore reagent ukuqinisekisa ukusebenza kokukhutshwa.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-3 RNase ukungcolan

- Nangona i-buffer ebonelelwe kwikhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngexesha lenkqubo yokukhupha kwaye kufuneka iphathwe ngononophelo.

Ungcoliseko lwe-4-Electrophoresis

---- Faka endaweni ye-electrophoresis buffer kwaye uqiniseke ukuba izinto ezisetyenziswayo kunye nokuLayisha iBuffer zikhululekile kungcoliseko lweRNase.

A-5 Ukulayisha kakhulu i-electrophoresis

---- Ukunciphisa inani lokulayisha isampula, ukulayishwa kwequla ngalinye akufuneki kudlule kwi-2 μg.

Umbuzo: Ungcoliseko lwe-DNA

A-1 Isixa sesampulu sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-2 Ezinye iisampulu zinomxholo ophezulu we-DNA kwaye zinokunyangwa nge-DNase.

- Yenza unyango lwe-RNase-Simahla ye-DNase kwisisombululo esifunyenweyo se-RNA, kwaye i-RNA inokusetyenziselwa ngokuthe ngqo kuvavanyo olulandelayo emva konyango, okanye inokucocwa ngakumbi ziikhithi zokucocwa zeRNA.

Q: Uyisusa njani i-RNase kwizinto ezisetyenziswayo zovavanyo nakwiglasi?

Ngeiglasi, kubhakwe kwi-150 ° C nge-4 h. Kwizikhongozeli zeplastiki, zibhaptizwe kwi-0.5 M NaOH kangange-10 min, emva koko zahlanjwa kakuhle ngamanzi angenasimahla e-RNase kwaye emva koko zibulale ukuze ususe i-RNase ngokupheleleyo. Izinto ezisetyenziswayo okanye isisombululo esisetyenzisiweyo kulingo, ngakumbi amanzi, kufuneka simahla kwi-RNase. Sebenzisa amanzi asimahla e-RNase kuwo onke amalungiselelo e-reagent (yongeza amanzi kwibhotile yeglasi ecocekileyo, yongeza i-DEPC kuxinzelelo lokugqibela lwe-0.1% (V / V), gungqisa ubusuku kunye ne-autoclave).

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