RNAprep Pure Plant Plus Kit Featured Image

RNAprep Isityalo esicocekileyo kunye neKhithi

Ukucoca i-RNA iyonke ukusuka kwiipolysaccharides kunye neesampulu zezityalo ezityebileyo.

I-RNAprep yePlanti yeZityalo eziSulungekileyo kunye nePolysaccharides kunye nePolyphenolics-sisityebi) sisicoci se-RNA sokucoca i-RNA kit eyenzelwe iisampulu zezityalo ezininzi ezine-polysaccharides kunye ne-polyphenolics. Inikwa nge-Buffer SL enobuchule obugqwesileyo be-lysis. Ubumsulwa obupheleleyo be-RNA ngaphandle kwe-gDNA bunokukhutshwa kwiinyama zebhanana, iivatala, iiapile, amapere, izilimo zeetapile, iitapile, kunye namagqabi omqhaphu, irose, ialfalfa, irayisi kunye neenaliti ezimhlophe zepine ngaphakathi kweyure enye .

Ikati. Hayi	Ubungakanani bokuPakisha
4992239	50 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

FAQ

Iimpawu zemveliso

Iimbonakalo

Tar Ithagethi: Yenzelwe ngokukodwa iisampulu zezityalo ekunzima ukuzikhupha, ezifana neepolysaccharides kunye nezityalo ezinepolyphenolics. Inkqubo ilungiselelwe ngakumbi, kwaye iziphumo zithembekile.
■ Ukususwa ngokufanelekileyo kwe-gDNA: Ukusebenza okuphezulu kwe-DNase I kunikezelwa ngokususwa ngokukhawuleza kwe-gDNA kwikholamu.
■ Kulula kwaye kuyakhawuleza: Uvavanyo lweRNA lokukhupha lunokugqitywa kwiyure enye.
■ Ityhefu ekhuselekileyo nephantsi: akukho zixhobo zinetyhefu yezinto eziphilayo ezinjenge phenol kunye ne chloroform ezifunekayo.

Izicelo

Le khithi inokusetyenziselwa ngokuthe ngqo kuvavanyo lwebhayoloji ezahlukeneyo ezinjenge-RT-PCR, i-Real-time PCR, iNorthern Blot, iDot Blot, ukuvavanywa kwePolyA, ukuguqulelwa kwe-vitro, uhlalutyo lokhuselo lwe-RNase, kunye nokwenza ikhonco, njl.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo RNALock Reagent

Okulandelayo: I-RNYenza i-Hi-Blood Kit emsulwa

Iyonke i-RNA ikhutshwe kwi-100 mg yenyama yebhanana, ivatala, iapile kunye neeperile, izilimo eziyizigaxa zebhatata kunye neetapile, amagqabi omqhaphu, irose, ialfalfa, irayisi kunye neenaliti ezimhlophe zepine ngokulandelanayo zisebenzisa iRNAprep Pure Plant Plus Kit. I-4-6 μl ye-30 μl eluates ilayishwe ngomgaqo ngamnye.
M: UMarki III weTIANGEN;
I-electrophoresis yenziwa nge-6 V / cm kwimizuzu engama-30 kwi-1% ye-agarose.
Iziphumo: I-RNAprep Pure Plant Plus Kit inokukhupha ubunyulu obuphezulu, isivuno esiphakamileyo kunye nokunyaniseka okupheleleyo kweRNA kwiipolysaccharides kunye neesampulu zezityalo ezityebileyo ze-polyphenolics.

Q: Ikholamu yokuvalela

I-A-1 Cell lysis okanye i-homogenization ayonelanga

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampuli esetyenzisiweyo okanye ukwandisa inani le-lysis buffer.

Q: Isivuno seRNA esisezantsi

A-1 I-lysis yeseli engonelanga okanye i-homogenization

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

- Nceda ubhekise kubungakanani bokusebenza.

I-A-3 RNA ayikhutshiwe ngokupheleleyo kwikholamu

--Ngasemva kokongeza amanzi asimahla e-RNase, yishiye imizuzu embalwa ngaphambi kokufaka isenti.

I-A-4 Ethanol elufeni

- Emva kokucoca, icentrifuge kwakhona kwaye ususe isikhuseli sokuhlamba kangangoko.

I-A-5 yesiko lenkcubeko yeseli ayisuswanga ngokupheleleyo

- Xa uqokelela iiseli, nceda uqiniseke ukuba ususa indawo yenkcubeko kangangoko kunokwenzeka.

A-6 Iiseli ezigcinwe kwiRNAstore azisebenzi kakuhle kwisenti

--Uxinano lweRNAstore lukhulu kunophakathi ophakathi kwenkcubeko yeseli; ke amandla e-centrifugal kufuneka anyuswe. Kuyacetyiswa kwi-centrifuge kwi-3000x g.

Umxholo we-A-7 Low RNA kunye nobuninzi kwisampulu

--Sebenzisa isampulu elungileyo ukumisela ukuba ngaba isivuno esisezantsi sibangelwa sisampulu.

Q: Ukuthotywa kweRNA

A-1 Imathiriyeli ayisiyonto intsha

Izicubu ezintsha kufuneka zigcinwe kwi-nitrojeni engamanzi kwangoko okanye kwangoko ibekwe kwi-RNAstore reagent ukuqinisekisa ukusebenza kokukhutshwa.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-3 RNase ukungcolan

- Nangona i-buffer ebonelelwe kwikhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngexesha lenkqubo yokukhupha kwaye kufuneka iphathwe ngononophelo.

Ungcoliseko lwe-4-Electrophoresis

---- Faka endaweni ye-electrophoresis buffer kwaye uqiniseke ukuba izinto ezisetyenziswayo kunye nokuLayisha iBuffer zikhululekile kungcoliseko lweRNase.

A-5 Ukulayisha kakhulu i-electrophoresis

---- Ukunciphisa inani lokulayisha isampula, ukulayishwa kwequla ngalinye akufuneki kudlule kwi-2 μg.

Umbuzo: Ungcoliseko lwe-DNA

A-1 Isixa sesampulu sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-2 Ezinye iisampulu zinomxholo ophezulu we-DNA kwaye zinokunyangwa nge-DNase.

- Yenza unyango lwe-RNase-Simahla ye-DNase kwisisombululo esifunyenweyo se-RNA, kwaye i-RNA inokusetyenziselwa ngokuthe ngqo kuvavanyo olulandelayo emva konyango, okanye inokucocwa ngakumbi ziikhithi zokucocwa zeRNA.

Q: Uyisusa njani i-RNase kwizinto ezisetyenziswayo zovavanyo nakwiglasi?

Ngeiglasi, kubhakwe kwi-150 ° C nge-4 h. Kwizikhongozeli zeplastiki, zibhaptizwe kwi-0.5 M NaOH kangange-10 min, emva koko zahlanjwa kakuhle ngamanzi angenasimahla e-RNase kwaye emva koko zibulale ukuze ususe i-RNase ngokupheleleyo. Izinto ezisetyenziswayo okanye isisombululo esisetyenzisiweyo kulingo, ngakumbi amanzi, kufuneka simahla kwi-RNase. Sebenzisa amanzi asimahla e-RNase kuwo onke amalungiselelo e-reagent (yongeza amanzi kwibhotile yeglasi ecocekileyo, yongeza i-DEPC kuxinzelelo lokugqibela lwe-0.1% (V / V), gungqisa ubusuku kunye ne-autoclave).

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