I-RNAprep Kit efanelekileyo yeeTishu

Ukucoca ukuya kuthi ga kwi-100 μg iyonke i-RNA kwizicubu zezilwanyana.

I-RNAprep Pure Tissue Kit ibonelela ngendlela ekhawulezayo, elula, kunye neendleko zokucoca i-RNA iyonke kwizicubu zezilwanyana ngokusebenzisa ikholamu efanelekileyo yokujikeleza kunye nenkqubo eyingqayizivele yesixhobo. Ikhithi ibandakanya i-RNase-Free spin iikholamu ze-CR3 zokucoca i-RNA ekumgangatho ophezulu ngokusebenzisa itekhnoloji ye-silica-membrane. Umgangatho opheleleyo we-RNA unokufunyanwa kwimizuzu engama-40-50 ngokusulungeka okuphezulu kwaye akukho simahla kwiprotein kunye ne-genomic DNA ungcoliseko.

Ikati. Hayi	Ubungakanani bokuPakisha
4992236	50 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

FAQ

Iimpawu zemveliso

Iimbonakalo

Buff Izilondolozi ezilungiselelwe izicwili zezilwanyana zenza ukuba inkqubo ibe lula kwaye ifaneleke.
■ I-DNase eyahlukileyo ndiyacutha ukungcoliseka kwe-genomic DNA.
■ Ukucoceka okuphezulu kunye nokulungele ukusetyenziswa kwe-RNA kufanelekile kwizicelo ezibuthathaka ezantsi.
■ Akukho kucolwa kwephenol / chloroform, akukho LiCl kunye nemvula ethanol, kwaye akukho CsCl gradients centrifugation efunekayo, eyenza inkqubo ikhuseleke kwaye ithembeke.

Izicelo

■ I-RT-PCR.
■ Iblukhwe laseMantla, iBala eliMachaphaza.
■ I-PCR yokwenene.
■ Uhlalutyo lwe-Chip.
■ Ukuvavanywa kwePolyA, kuguqulelo lwe-vitro, uhlalutyo lokhuselo lwe-RNase kunye nokwenziwa kwemolekyuli.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo RNAprep Isisele esinyulu / iBacteria Kit

Okulandelayo: RNAprep Pure FFPE Kit

Isixhobo: 20 mg umbungu (iintsuku ezili-13), izintso ezili-15 mg, isibindi esingu-10 mg, udakada weshumi mg, 10 mg thymus, 20 mg lung
Indlela: Iyonke i-RNA evela kwiisampulu ezahlukeneyo zethini yacocwa kusetyenziswa i-RNAprep Tissue Kit.
Iziphumo: Umfanekiso we-agarose gel electrophoresis uboniswe apha ngasentla. I-2-4 μl ye-100 μl eluates ilayishwe ngomgaqo ngamnye.
M: ICANDELO LE-DNA Phawu III;
Umgaqo 1-2: Umbungu (iintsuku ezili-13); Umgaqo 3: Izintso; Umgaqo 4-6: Isibindi; Umgaqo 7: Udakada; Umgaqo 8: Thymus; Umgaqo 9: Umphunga.
I-electrophoresis yenziwa nge-6 V / cm kwimizuzu engama-30 kwi-1% yejeli ye-agarose ..

Q: Ikholamu yokuvalela

I-A-1 Cell lysis okanye i-homogenization ayonelanga

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampuli esetyenzisiweyo okanye ukwandisa inani le-lysis buffer.

Q: Isivuno seRNA esisezantsi

A-1 I-lysis yeseli engonelanga okanye i-homogenization

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

- Nceda ubhekise kubungakanani bokusebenza.

I-A-3 RNA ayikhutshiwe ngokupheleleyo kwikholamu

--Ngasemva kokongeza amanzi asimahla e-RNase, yishiye imizuzu embalwa ngaphambi kokufaka isenti.

I-A-4 Ethanol elufeni

- Emva kokucoca, icentrifuge kwakhona kwaye ususe isikhuseli sokuhlamba kangangoko.

I-A-5 yesiko lenkcubeko yeseli ayisuswanga ngokupheleleyo

- Xa uqokelela iiseli, nceda uqiniseke ukuba ususa indawo yenkcubeko kangangoko kunokwenzeka.

A-6 Iiseli ezigcinwe kwiRNAstore azisebenzi kakuhle kwisenti

--Uxinano lweRNAstore lukhulu kunophakathi ophakathi kwenkcubeko yeseli; ke amandla e-centrifugal kufuneka anyuswe. Kuyacetyiswa kwi-centrifuge kwi-3000x g.

Umxholo we-A-7 Low RNA kunye nobuninzi kwisampulu

--Sebenzisa isampulu elungileyo ukumisela ukuba ngaba isivuno esisezantsi sibangelwa sisampulu.

Q: Ukuthotywa kweRNA

A-1 Imathiriyeli ayisiyonto intsha

Izicubu ezintsha kufuneka zigcinwe kwi-nitrojeni engamanzi kwangoko okanye kwangoko ibekwe kwi-RNAstore reagent ukuqinisekisa ukusebenza kokukhutshwa.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-3 RNase ukungcolan

- Nangona i-buffer ebonelelwe kwikhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngexesha lenkqubo yokukhupha kwaye kufuneka iphathwe ngononophelo.

Ungcoliseko lwe-4-Electrophoresis

---- Faka endaweni ye-electrophoresis buffer kwaye uqiniseke ukuba izinto ezisetyenziswayo kunye nokuLayisha iBuffer zikhululekile kungcoliseko lweRNase.

A-5 Ukulayisha kakhulu i-electrophoresis

---- Ukunciphisa inani lokulayisha isampula, ukulayishwa kwequla ngalinye akufuneki kudlule kwi-2 μg.

Umbuzo: Ungcoliseko lwe-DNA

A-1 Isixa sesampulu sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-2 Ezinye iisampulu zinomxholo ophezulu we-DNA kwaye zinokunyangwa nge-DNase.

- Yenza unyango lwe-RNase-Simahla ye-DNase kwisisombululo esifunyenweyo se-RNA, kwaye i-RNA inokusetyenziselwa ngokuthe ngqo kuvavanyo olulandelayo emva konyango, okanye inokucocwa ngakumbi ziikhithi zokucocwa zeRNA.

Q: Uyisusa njani i-RNase kwizinto ezisetyenziswayo zovavanyo nakwiglasi?

Ngeiglasi, kubhakwe kwi-150 ° C nge-4 h. Kwizikhongozeli zeplastiki, zibhaptizwe kwi-0.5 M NaOH kangange-10 min, emva koko zahlanjwa kakuhle ngamanzi angenasimahla e-RNase kwaye emva koko zibulale ukuze ususe i-RNase ngokupheleleyo. Izinto ezisetyenziswayo okanye isisombululo esisetyenzisiweyo kulingo, ngakumbi amanzi, kufuneka simahla kwi-RNase. Sebenzisa amanzi asimahla e-RNase kuwo onke amalungiselelo e-reagent (yongeza amanzi kwibhotile yeglasi ecocekileyo, yongeza i-DEPC kuxinzelelo lokugqibela lwe-0.1% (V / V), gungqisa ubusuku kunye ne-autoclave).

Bhala umyalezo wakho apha uze uyithumele kuthi