■ Inqanaba lokubuyisa le-100 bp-10 kb yeqhekeza le-DNA linokufikelela ngaphezulu kwe-80%.
The Elona xabiso liphezulu le-DNA yentengiso kwiqula ngalinye yi-5 µg.
■ Ukucoceka okuphezulu: I-DNA esulungekileyo inokusetyenziswa ngokuthe ngqo kuvavanyo olusezantsi.
Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)
Ama-900 bp, ama-3500 bp kunye nama-4500 amaqhekeza e-bp ahlanjululwa kusetyenziswa i-TIANquick N96 Purit Kit.
I-3 μl ye-50 μl eluant yafakwa kwi-1% ye-agarose gel, kunye ne-electrophoresis yenziwa nge-6 V / cm kwimizuzu engama-20.
1, 2 no-3: I-DNA ihlanjululwe kusetyenziswa i-TIANGEN TIANquick N96 Kit Yokuhlanjululwa;
4: I-DNA iyacocwa kusetyenziswa imveliso efanelekileyo evela kuMthengisi A.
Oko yasekwa, umzi-mveliso wethu uphuhlisa iimveliso zodidi lokuqala lwehlabathi ngokubambelela kumgaqo
esemgangathweni kuqala. Iimveliso zethu ziye zaziwa ngokugqwesileyo kushishino kunye nexabiso lokuthenjwa phakathi kwabathengi abatsha nabadala ..