Inkqubo yeTeasy AP 400/600 yokuZenzekelayo

Ukuhamba okuphezulu, imibhobho ezenzekelayo.

Inkqubo yokuPeyinta ngokuZenzekelayo iyinkqubo ezenzekelayo kunye nocoselelo oluphezulu. Isetyenziswa ngokukodwa ukulungiselela inkqubo encinci ye-PCR / qPCR, enokuthi ithathe indawo yokulungiselela ngesandla kwePCR / qPCR. Inkqubo inokuqinisekisa ukuchaneka, ukuchaneka nokuphindeka kovavanyo, kunye nokunciphisa iimpazamo zokusebenza ngesandla. Okwangoku, i-TEasy AP 400/600 Inkqubo yokuPaka ngokuZenzekelayo inokuxhotyiswa ngesibane se-UV kunye ne-HEPA, kwaye inokusetyenziselwa ukuhanjiswa kolwelo kwinkcubeko yeseli.

Ikati. Hayi	Ubungakanani bokuPakisha
I-OSE-AP400	1 Cwangcisa
I-OSE-AP600	1 Cwangcisa

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

Iimpawu zemveliso

Iparameters zokusebenza

TEasy AP 400/600 Automated Pipetting System

Ukuxhasa iibhloko

TEasy AP 400/600 Automated Pipetting System

Iimbonakalo

■ Kulula ukuyisebenzisa: Umsebenzi wesoftware unokufumana ngokulula kwi-1 h. Inkqubo eyakhelweyo yokwakha ye-PCR / qPCR inokulungiswa kwaye ihanjiswe ngokukhawuleza.
■ Izinto ezisetyenziswayo ezihambelanayo: Ingcebiso yepipette etshintshayo kunye nenkqubo yeBeckman Biomek 3000.
■ Ulondolozo olulula: Imodyuli yombhobho wokuzenzekelayo (i-APM) inokutshintshwa ngokulula kwaye ibuyiselwe ukulungisa ingxaki.
■ Ukuchaneka okuphezulu nokuchaneka.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-TGreen Transilluminator

Okulandelayo: I-TGrade Dry Bath Incubator

TEasy AP 400/600 Automated Pipetting System

Umzobo 1: Iziphumo zegophe eziqhelekileyo ze-qPCR zibonisa ukuphindeka okuhle
Iisampulu ze-cDNA ze-7 μl NIH 3T3 iiseli zixutywe amaxesha ama-4 nge-21 μl yamanzi kumyinge we-1: 4. IRoche LightCycler 480 yokwenene yexesha le-fluorescence yesixhobo sePCR kunye neSuperReal PreMix Plus (SYBR Green) zazisetyenziselwa ukufumanisa.

Umzobo 2: Ukuchaneka okuphezulu xa kuthelekiswa nombhobho wokubhala
(Ekhohlo: Imanyuwali; Ekunene: I-TEasy Pipetting)
Ukuphindwaphindwa okungu-4 kolwaziso lomntu lwe-GAPDH (ijika ephezulu). Yongeza i-2 μl cDNA kwi-18 μl MasterMix ukwenza inkqubo ye-20 μl yokuphendula. I-Roche LightCycler 480 yokwenene yexesha le-PCR yesixhobo kunye ne-SuperReal PreMix Plus (i-SYBR Green) yayisetyenziselwa ukufumanisa.

Bhala umyalezo wakho apha uze uyithumele kuthi