■ Imowudi yokuhamba-ntathu: Amandla okusila e-homogenization amandla aphindwe kabini ukuya kwezi-5 kwizixhobo zemveli, kwaye i-homogenization yokugaya ihamba ngesantya esiphezulu kunye nokusebenza kakuhle.
■ Isetyhula yoyilo lwesiphatho setyhula: Isiphumo sokuguba okungafaniyo ngaphandle kongcoliseko lomnqamlezo.
■ Uyilo olukhethekileyo kunye noyilo lokunciphisa ingxolo: Izixhobo zesixhobo akukho lula ukunxiba, kwaye ingxolo ebalekayo iyancitshiswa ukuqinisekisa amava omsebenzisi angcono.
■ Isixhobo sokukhusela ngokuzenzekelayo: Xa isiciko sokhuselo singavaliwe, isixhobo asinakuqala okanye siqhekeze ngexesha likaxakeka, ukuze sikhusele abasebenzi baselebhu.
Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)
Ukugaya kunye ne-homogenization yeesampulu zezilwanyana / zezityalo kunye nokukhutshwa kwe-genomic ye-DNA 1. Manual ulwelo nitrogen lokusila, isibindi ibuzi; 2: TGrinder H24, isibindi somlenze; 3. Manual ulwelo nitrogen yokuguba, intliziyo yeempuku; 4: TGrinder H24, intliziyo yempuku; 5. Ukusila incindi yenitrojeni ngesandla, igqabi lengqolowa; 6: TGrinder H24, igqabi lengqolowa. I-genomic DNA ye-20 mg yezicubu zezilwanyana kunye ne-10 mg yezicubu zezityalo zakhutshwa nge-nitrogen engamanzi kunye ne-TGrinder H24 yokugaya kunye ne-homogenizing ngokwahlukeneyo. Ukuveliswa kwe-genomic ye-DNA yeesampulu kwakulingana ngokulinganayo. |
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Ukugaya kunye nokwenza homogenization kweesampulu zomhlaba kunye nokukhutshwa kwe-genomic ye-DNA 1: Ukuhlamba ibhafu yentsimbi; 2: IGrinder H24. I-genomic ye-DNA ye-0.25 g yeesampulu zomhlaba yakhutshwa ngokushukumisa ibhafu yentsimbi kunye ne-TGrinder H24 ngokwahlukeneyo Isivuno se-genomic DNA ngu-TGrinder H24 sasiphezulu kakhulu, kwaye ixesha lokuphinda kuthathwe iisampulu lalisezantsi kakhulu (ukushukumisa ibhafu yentsimbi-2000 rpm 10 min, TGrinder H24-6 m / s 30 s 2 cycles). |
Oko yasekwa, umzi-mveliso wethu uphuhlisa iimveliso zodidi lokuqala lwehlabathi ngokubambelela kumgaqo
esemgangathweni kuqala. Iimveliso zethu ziye zaziwa ngokugqwesileyo kushishino kunye nexabiso lokuthenjwa phakathi kwabathengi abatsha nabadala ..