EndoFree Maxi Plasmid Kit Featured Image

Ikhithi ye-EndoFree Maxi Plasmid

Ukucocwa kwebakala le-plasmid DNA engenasiphelo.

I-EndoFree Maxi Plasmid Kit isekwe kubuchwephesha be-silica membrane ukukhupha i-endotoxin-free plasmid DNA. Le khithi ibonelela ngenkqubo yokususa i-endotoxin yokuvelisa i-plasmid DNA ekumgangatho ophezulu kwizicelo ezisezantsi. Yenzelwe ukwahlula i-DNA yeplasmid yokusulungeka okuphezulu ukusuka kwi-100-200 ml yenkcubeko yebhaktiriya kwaye inokuvelisa ukuya kuthi ga kwi-1.5 mg ye-plasmid grade ye-DNA.

Ikati. Hayi	Ubungakanani bokuPakisha
4992194	10 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

Yield Isivuno esikhawulezayo nesiphezulu: I-200 μg-1.5 mg yeplasmid isivuno seDNA kwiyure e-1 enomlinganiselo ophezulu wolwakhiwo olugqwesileyo.
■ Ukucoceka okuphezulu: I-plasmid DNA esulungeke kakhulu ifunyanwa yinkqubo eyodwa ye-buffer kunye ne-Spin Column CP6.
■ Ukusebenza ngokugqwesileyo kosulelo: Kukulungele ukuvavanywa kokukhutshelwa kweeselfowuni.
■ Uluhlu olubanzi lwezicelo: Ilungele ukuthintela ukwetyisa i-enzyme, ukutshintsha, ukulandelelana, i-microinjection, ukuthulisa imfuza kunye nokuvavanywa kokuhanjiswa.

Ukuveliswa kwePlasmid

Plasmid Yield

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-TIANprep Rapid N96 Plasmid Kit

Okulandelayo: I-Mini Eplasmid Kit II engapheliyo

Ikopi ephezulu / esezantsi yokukhutshwa kweplasmid
Ikopi ephantsi yeplasmid pBR322, ekhutshwe kwi-200 ml yenkcubeko yebhaktiriya isebenzisa i-EndoFree Maxi Plasmid Kit, yaxutywa kwi-1 ml Buffer TB, ene-0.6 μg / μl. Ikopi ephezulu ye-plasmid pBS, ekhutshwe kwi-100 ml yenkcubeko yebhaktiriya isebenzisa i-EndoFree Maxi Plasmid Kit, yaxutywa kwi-1 ml Buffer TB, ene-1.2 μg / μl.
I-MIV: I-TIANGEN yeDNA ephawulayo IV
pBR322: 2 μl, pBS: 2 μl

I-pEGFP efunyenwe nge-EndoFree Maxi Plasmid Kit yahanjiswa ngokwahlukeneyo yaya kwi-endotoxin-insensitive cell line 293T kunye ne-endotoxin-sensitive cell line line Huh7 yi-TIANGEN TIANfect. Inkcazo ye-GFP yafunyanwa kwiiyure ezingama-24 emva kokudluliselwa.

Bhala umyalezo wakho apha uze uyithumele kuthi