Ikhithi ye-EndoFree Maxi Plasmid V2

Ukucocwa kwe-endotoxin-free transfection grade plasmid DNA ekhethekileyo yeeseli ezibuthathaka.

I-EndoFree Maxi Plasmid Kit V2 yamkela ubuchule obukhethekileyo be-silica membrane adsorption ngokuchanekileyo nangokukhethekileyo ibopha iplasmid DNA. I-Buffer ER ekhethekileyo kunye ne-Filtration CS1 zinokususa ngokufanelekileyo ukungcola okunje nge-endotoxin kunye neprotein. Yonke inkqubo yokukhupha ithatha iyure enye.
Kucetyiswa inani eliphakathi lenkcubeko yebhaktiriya: Kwikopi ephezulu yeplasmid, kucetyiswa i-100 ml yebhaktiriya ephakathi, enokuvelisa ukuya kuthi ga kwi-500-1500 μg plasmid. Kwikopi ephantsi yeplasmid, 200 ml yeebhaktiriya eziphakathi kucetyiswa ukufezekisa isivuno se-50-300 μg.

Ikati. Hayi	Ubungakanani bokuPakisha
4992438	10 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Ukuhamba komsebenzi

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

■ Ukucoceka okuphezulu: Itekhnoloji ekhethekileyo yokutsalwa kwe-endotoxin yamkelwe ukususa ngokukodwa i-endotoxin.
■ Kulula ukusebenza: Itekhnoloji yekholamu yokuthengisa isetyenziselwa ukukhangela ngokuthe ngqo i-plasmid DNA, eyenza umsebenzi ube lula.
Trans Ukonyuka kokusebenza okuphezulu: Kukulungele ukutshintshwa kweeseli ezininzi kubandakanya iiseli ezibuthathaka kwi-endotoxin.
■ Uluhlu olubanzi lwezinto ezisetyenziswayo: Iplasmid ecociweyo inokusetyenziswa kugqithiso lweeseli zezilwanyana nezityalo kunye nolingo lwebhayoloji yemolekyuli.

Izicelo

I-plasmid DNA ekhutshwe kusetyenziswa le khithi inokusetyenziselwa imisebenzi eyahlukeneyo yesiqhelo, kubandakanya ukuthintela ukwetyisa i-enzyme, i-PCR, ukulandelelana, ukudibanisa, ukuguqula kunye nokuhambisa iiseli ezahlukeneyo.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-TIANprep N96 Magnetic Plasmid Kit

Okulandelayo: I-STIANqucik N96 Kit yokuCoca

Ukususwa okukodwa kwe-endotoxin reagent Buffer ER kungazisusa ngokufanelekileyo iintsalela ze-endotoxin kwinkqubo yokuphendula, kunye neplasmid yokucoceka okuphezulu inokufumaneka. Intsalela yeplasmid endotoxin yi ≤ 0.1 EU / ml.

Iplasmid ecociweyo kusetyenziswa i-EndoFree Maxi Plasmid Kit V2 kunye neemveliso ezifanayo ezivela kuMboneleli 1 kunye noMthengisi 2 zikhutshiwe ngevolumu efanayo ye-elution buffer. I-1 μl plasmid ilayishwe ngomgaqo ngamnye ukuqikelela uxinzelelo lweplasmid. I-100 ng ye-plasmid egxininiswe ngo-spectrophotometer yafakwa kwi-gel efanayo ukumisela ukuba ixabiso loxinzelelo laliphezulu ngokuxoka.
Isiphelo: Isiphumo se-electrophoresis sibonisa eyona plasmid ikhishwe yi-EndoFree Maxi Plasmid kit V2, nokuba ikopi ephezulu okanye ikopi ephantsi, iphezulu kakhulu kunaleyo ikhutshwe ngabanikezeli nge-1 kunye ne-2.

Bhala umyalezo wakho apha uze uyithumele kuthi