I-TIANamp FFPE ID Kit

Ukucoca okuphezulu okusebenzayo kwe-DNA evela kwizicwili ezinikezelweyo ngokusesikweni, iiparafini ezifakwe kunyango lwe-xylene.

I-TIANamp FFPE DNA Kit yenzelwe ukucocwa kwe-DNA kumacandelo e-FFPE tissue. Isebenzisa i-xylene ukususa iparafini, kwaye ibonelela ngeemeko ezizodwa zelisisi ukuze ikhutshwe yi-DNA kwisilayidi sesicwili. Idityaniswe nokukhetha-okubopha inwebu esekwe kwisilika kunye nenkqubo yokuguquguquka okuguqukayo, le khithi inokukhupha i-DNA ekumgangatho ophezulu ukuya kumthamo omncinci. I-DNA ehlanjululweyo inokusebenza ngokuthe ngqo njengeetemplate zokufumana ubukho bezantsi okanye ezinye iinkqubo.

Ikati. Hayi	Ubungakanani bokuPakisha
4992524	50 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

FAQ

Iimpawu zemveliso

Iimbonakalo

■ Ukucoceka okuphezulu: I-Silica membrane isusa konke ukungcola.
■ Ukusetyenziswa okubanzi: Ilungele icandelo leparafini, ibhloko yeparafini, kunye neesampulu ezifakwe kwi-formalin.
Operation Umsebenzi okhawulezayo: I-DNA inokufunyanwa kwisithuba seyure enye.

Ukucaciswa

Uhlobo: Spin ikholamu esekwe
Isampulu: Icandelo leparafini, ibhloko yeparafini, kunye neesampulu ezifakwe kwi-formalin
Ithagethi: IGenomic DNA
Ukuqala kwevolumu: iziqwenga ezi-5-8 zamacandelo eparafini okanye izicubu ezingama-30 mg kwiparafini okanye kwi-formalin
Ixesha lokusebenza: ~ 1 iyure
Izicelo ezisezantsi: I-PCR, uhlalutyo lwe-SNP genotype, uhlalutyo lwe-STR, uhlalutyo lwe-pharmacogenomics, ulwakhiwo lwethala leencwadi le-NGS, njl.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-TIANquick FFPE DNA Kit

Okulandelayo: I-TIANprep N96 Plasmid Kit

I-genomic DNA ekhutshwe yi-TIANamp FFPE DNA Kit kunye nemveliso efanelekileyo kuMthengisi A yandiswa ukuya kwi-190 bp, 330 bp, 500 bp, 650 bp kunye ne-1300 bp. Iziphumo zibonisa ukuba iDNA ekhutshwe yi-TIANamp FFPE DNA Kit inesivuno esiphezulu, ubunyulu obuphezulu ngakumbi kumaqhekeza amakhulu.

Ukuqala kwezinto: Imouse iisampulu zesibindi se-FFPE (iziqwenga ezi-5, 10 μm / isiqwenga)
Inkqubo: Landela imiyalelo yemveliso nganye.
Ukulayishwa kwe-Gel: i-gDNA yaphakanyiswa nge-50 μl elution buffer kwaye yandiswa kwi-190 bp, 330 bp 500 bp, 650 bp kunye ne-1300 bp. I-5 μl ye-20 μl Iimveliso ze-PCR zilayishwe ngomgaqo ngamnye kwi-1% ye-agarose gel. I-electrophoresis yaqhutywa nge-6 V / cm kwimizuzu engama-20.
M: Isiphawuli seTIANGEN D2000;
Isixhobo se-TIANamp FFPE DNA;
A: Imveliso efanelekileyo evela kuMthengisi A.

Umbuzo: Incinci okanye ayikho i-DNA kwi-eluent.

A-1 Ukuxinana kweeseli okanye intsholongwane kwisampulu yokuqala -Tyebisa uxinzelelo lweeseli okanye iintsholongwane.

A-2 I-lysis engonelanga yeesampulu -Iisampulu azange zixutywe kakuhle kunye ne-lysis buffer. Kucetyiswa ukuba udibanise ngokucofa i-vortexing kangangezihlandlo ezi-1-2. -I-lysis yeseli engonelanga ebangelwe kukuncipha kwemisebenzi ye-proteinase K. Kucetyiswa ukuba usike izicwili zibe ziingceba kwaye wandise ixesha lokuhlamba ukususa yonke intsalela yelysate.

A-3 Ukungoneliseki kwe-DNA adsorption. -Ayikho i-ethanol okanye ipesenti esezantsi endaweni ye-100% ye-ethanol eyongezwe ngaphambi kokuba kudluliselwe i-lysate kwikholamu yokujikeleza.

A-4 Ixabiso le-pH ye-elution buffer liphantsi kakhulu. Lungisa i-pH iye phakathi kwe-8.0-8.3.

Q: I-DNA ayisebenzi kakuhle kulingelo lokuphendula kwe-enzymatic reaction.

Intsalela ye-ethanol kwi-eluent.

-Kukho intsalela yokuhlambela i-PW kwisikali. I-ethanol ingasuswa ngokufaka phakathi kwikholamu ejikelezayo ye-3-5 min, kwaye emva koko ubeke kubushushu begumbi okanye kwi-50 ℃ incubator ye-1-2 min.

Q: Ukonakaliswa kweDNA

A-1 Isampulu ayisiyonto intsha. -Khupha isampulu yeDNA elungileyo njengokulawula ukumisela ukuba ingaba iDNA ekwisampulu ihlaziyiwe.

Unyango lwangaphambi kokunyanga okungalunganga. -Yenziwe kukugaya i-nitrogen engamanzi ngokugqithisileyo, ukubuyisa ukufuma, okanye isixa esikhulu sesampulu.

Umbuzo: Uyenza njani inyanzelo yokukhutshwa kwe-gDNA?

Ukufakwa kwangaphambili kufuneka kwahluka kwiisampulu ezahlukeneyo. Kwiisampulu zezityalo, qiniseka ukuba usile kakuhle kwi-nitrogen engamanzi. Kwiisampulu zezilwanyana, homogenate okanye ugaye kakuhle kwi-nitrogen engamanzi. Kwiisampulu ezineendonga zeseli ekunzima ukuziqhekeza, ezinjenge-G + bacteria kunye nemvubelo, kucetyiswa ukuba kusetyenziswe i-lysozyme, i-lyticase okanye iindlela zoomatshini zokwaphula iindonga zeseli.

Q: Nguwuphi umahluko phakathi kweekiti ezintathu zokukhupha i-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 ye-Genomic DNA Kit isebenzisa indlela esekwe kwikholamu efuna ikloroform yokukhupha. Ifanelekile ngakumbi kwiisampulu ezahlukeneyo zezityalo, kunye nomgubo owomileyo wezityalo. I-Hi-DNAsecure Plant Kit nayo isekwe kwikholamu, kodwa kungekho mfuneko yokukhutshwa kwe-phenol / chloroform, ukuyenza ikhuseleke kwaye ingabinetyhefu. Kufanelekile ukuba izityalo ezine-polysaccharides ephezulu kunye nomxholo we-polyphenol. 4992709/4992710 DNAquick Plant System yamkela indlela esekwe kulwelo. Ukukhutshwa kwePhenol / chloroform akufuneki nako. Inkqubo yokuhlanjululwa ilula kwaye iyakhawuleza ngaphandle komda kwiisampulu zezixa zokuqala, ke abasebenzisi banokuhlengahlengisa isixa ngokuhambelana neemfuno zovavanyo. Ubungakanani obukhulu bamaqhekeza e-gDNA anokufunyanwa ngesivuno esikhulu.

Sesiphi isivuno esiqikelelweyo se-gDNA kwi-1 ml yesampulu yegazi yi-TIANamp Blood DNA Kit?

I-genomic DNA yakhutshwa kwimilinganiselo eyahlukeneyo yeesampulu zabantu zegazi yi-TIANamp Blood DNA Kit. Iziphumo zilandelayo. Iziphumo zidweliswe njengereferensi kuphela, ezona ziphumo zokukhupha zixhomekeke kwimeko yeesampulu.

Q: Ngaba i-4992207/4992208 kunye ne-4992722/4992723 ingasetyenziselwa ukukhupha i-DNA yegazi?

Ukukhutshelwa kwegazi le-DNA kungena kusetyenziswa ii-reagents ezibonelelwe kwezi khithi zimbini ngokutshintsha nje umthetho olandelwayo kumyalelo othile wokukhutshwa kwegazi le-DNA. Ikopi ethambileyo yenkqubo yokutsalwa kwegazi kwi-DNA inokukhutshwa xa uceliwe.

Q: Xa ufaka isicelo se-TIANamp Genomic DNA Kit, ungaqhekeza njani izicwili ezitsha ekumisweni kweseli?

Misa isampulu entsha nge-1 ml ye-PBS, isaline eqhelekileyo okanye i-TE buffer. Ngokupheleleyo homogenize isampuli yi homogenizer kwaye uqokelele isitshisi ukuya ezantsi kumbhobho nge-centrifuging. Yilahle into engaphaya kwamandla, kwaye uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde wenziwe khona nge-200 μl buffer GA. Ukucocwa kwe-DNA elandelayo kunokwenziwa ngokomyalelo.

Q: Uyikhetha njani imveliso yokukhutshwa kwe-DNA kwiplasma, i-serum kunye neesampulu zamanzi omzimba?

Ukucoca i-gDNA kwi-plasma, i-serum kunye neesampuli zomzimba, kuyacetyiswa i-TIANamp Micro DNA Kit. Ukucoca intsholongwane gDNA kwiisampulu ze-serum / plasma, I-TIANamp Virus DNA / RNA Kit iyacetyiswa. Ukucoca i-gDNA yebhaktiriya kwiisampulu ze-serum kunye neplasma, i-TIANamp Bacteria DNA Kit iyacetyiswa (i-lysozyme kufuneka ibandakanywe kubhaktiriya). Kwiisampulu zamathe, iHi-Swab DNA Kit kunye ne-TIANamp Bacteria DNA Kit ziyacetyiswa.

Q: Ungazikhetha njani iikiti ze-gDNA zokukhupha kwiisampulu zomngundo?

I-DNAsecure Plant Kit okanye i-DNAquick Plant System iyacetyiswa ukuba isuswe kwi-fungal genome extraction. Ukukhutshwa kwegwele le-genome, i-TIANamp Yeast DNA Kit iyacetyiswa (i-lyticase kufuneka izilungiselele).

Iselfowuni eDna yokuSusa iKhithi