I-TIANprep N96 Plasmid Kit

Ukujongwa okwenyani kwexesha le-lysis kunye nokutsalwa okuphezulu kweplasmids

Inwebu ye-N96 Plate CP3 kule khithi yamkela inoveli ye-silica matrix material eyenziwe ngokukodwa yi-TIANGEN, enokuthi isebenze ngokufanelekileyo kwaye ngokuthe ngqo ithengise i-plasmid DNA, isuse iiprotein kunye nokunye ukungcola ukuya kuthi ga ngoku, ngaloo ndlela kuqinisekiswe ubumsulwa beplasmid ekhutshiweyo. Ukuya kuthi ga kwi-1.3 ml yekopi yeplasmid yenkcubeko yebhaktiriya ephezulu inokucutshungulwa kakuhle, ukusuka apho ukuya kuthi ga kwi-5-10 μg ye-plasmid DNA inokukhutshwa ngokukhawuleza (isivuno sinxulumene nezinto ezinjengeenombolo zeplasmid, imeko yebhaktiriya, njl. ). I-Plasmid DNA ekhutshwe yikhithi inokusetyenziswa kuvavanyo lwebhayoloji njengokuthintela ukwetyisa i-enzyme, ukulandelelana, ukuvavanywa kwethala leencwadi, ukubopha kunye notshintsho.

Ikati. Hayi	Ubungakanani bokuPakisha
4992892	4 ii-preps
4992893	24 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

■ Isantya sokukhupha ngokukhawuleza.
■ Exhotywe nge-reagent ekhethekileyo ye-TIANRed, inokuchonga ngokucacileyo inqanaba le-lysis yesampulu kwinkqubo yokutsala ukuqinisekisa ukusebenza okuphezulu kunye nokucoceka okuphezulu kwe-DNA yeplasmid.
■ Ukucoceka okuphezulu: Iplasmid DNA esulungekisiweyo ingasetyenziswa ngokuthe ngqo kumalinge asezantsi.
This Le khithi inokusetyenziselwa indawo yokusebenza efumba phezulu ngokuzenzekelayo.

Isalathi seTIANRed Colour

TIANRed Color indicator:

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-TIANamp FFPE ID Kit

Okulandelayo: I-TIANamp yegazi ye-DNA Midi Kit

	I-Plasmid DNA yatsalwa kwi-1 ml yobusuku obukhulisiweyo be-Escherichia coli (TOP10) (LB medium) isebenzisa i-TIANprep Highpure N96 Plasmid Kit, kunye ne-avareji ye-5-6 μg yokucoceka okuphezulu kwe-pBS plasmid yafunyanwa. I-plasmid yalayishwa kwiqula ngalinye le-1% yejeli ye-agarose, kwaye yafunyanwa yi-electrophoresis
	I-Plasmid DNA yatsalwa kwi-1 ml yobusuku obukhulisiweyo be-Escherichia coli (TOP10) (LB medium) isebenzisa i-TIANprep Highpure N96 Plasmid Kit, kunye ne-avareji ye-5-6 μg yokucoceka okuphezulu kwe-pBS plasmid yafunyanwa. Vector: i-pBS; Uxinzelelo lweplasmid: TOP10. I-plasmid yalandelwa yi-ABI. I-PRISM 3730XL ye-DNA sequencer.