Ukukhawulezisa inyathelo elinye kwi-RT-qPCR kit

Inyathelo elinye le-Real-Time RT-PCR ene-SYBR-Green.

I-FastKing Inyathelo elinye i-RT-qPCR Kit yenzelwe iXesha leXesha leNene nge-RT-qPCR ngokusebenzisa i-SYBR Green I. Ikhithi inokusetyenziswa ngexesha langempela le-RT-qPCR yeethagethi ze-RNA, ngokubhala umva kunye nePCR kwenzeka ngokulandelelana kumbhobho omnye. Ke ngoko umsebenzi ulula, kwaye unciphisa umngcipheko wongcoliseko.

Umxube we-25 × RT-PCR we-Enzyme kule khithi sisiqalo se-TIANGEN's inoveli yokubuyisela umva ngokutsha (i-FastKing RTase), inoveli yomntu olungisiweyo otshisayo Taq I-DNA polymerase kunye ne-RNase Inhibitor. I-FastKing RTase luhlobo olutsha lweemolekyuli eziguqulweyo. Ngokudityaniswa ngokukodwa kwe-hydrophobic motif, ene-RNA eyomeleleyo kunye nokuzinza kwe-thermal. I-FastKing RTase iphucule ukubuyela umva kokukhutshelwa kunye nokukwazi ukwandisa itemplate yeRNA ngesakhiwo esimbaxa sesibini. Uhlobo olutsha lwe hotstartTaq I-DNA polymerase enentsebenzo egqwesileyo isetyenziswa kwinkqubo ye-PCR, isenza impendulo ye-PCR emva kokukhutshelwa okubuyela umva kube nokusebenza okuphuculweyo kunye nokucaciswa. Ukongeza, i-2 × FastKing RT-qPCR Buffer (SYBR Green) kule mveliso yinkqubo entsha yokuphendula elungiselelwe ezi enzymes zimbini zingasentla, kubandakanya izinto eziyimfuneko ze-ionic, ii-dNTPs, idayi yeGreen Green, inyathelo elinye lokuzinzisa kunye nesomelezi, unokuqinisekisa ukuba i-FastKing RTase kunye ne-hotstart entshaTaq I-DNA polymerase inokudlala eyona ndima isebenzayo kuyo yonke inkqubo yokuphendula inyathelo elinye.

Le khithi ibonelela ngee curve ezifanelekileyo kumgangatho obanzi wobungakanani bendawo. Inokuchaneka ngokuchanekileyo nangokuchanekileyo ubuninzi beentlobo eziphezulu kunye nezisezantsi zokujolisa ekujoliseni ukuphindeka okuhle kunye nokuthembeka okuphezulu.