■ Imo eyahlukileyo yentsimbi yemagneti yokungcangcazela ine-amplitude enkulu yokungcangcazela kunye nokuchaneka okuphezulu.
■ Imowudi yokuthengisa eyomeleleyo entsha, ukusebenza kakuhle kwamaso ombane kunye nesiphumo seasidi ephezulu yenyukliya.
■ Ukunciphisa umsebenzi wokukhusela indawo, ukusebenza okuzinzileyo kunye nokuhleleka kwesixhobo ukunqanda ukusilela.
■ I-Windows Pad kunye neqhosha lesikrini kwimowudi yolawulo oluphindwe kabini, umsebenzi wokuhlela inkqubo onamandla, umsebenzi onomdla, olula nolula.
■ Ukungcoliseka kwenkqubo yolawulo olukrelekrele kunye nemodyuli yokubulala inzala ye-UV inokukuthintela ukungcoliseka komnqamlezo phakathi kwequla kunye neebhetshi.
■ Ukuthembela kwi-TIANGEN ye-asidi ye-asidi yokukhutshwa kunye neqonga le-reagent ye-reagent, inika isisombululo esibanzi, esigqibeleleyo nesikumgangatho ophezulu.
Emva kokuba isampulu ifakwe lishe kwi-buffer ye-lysis, i-acid ye-nucleic eyahluliweyo kwisisombululo se-lysis / sokubopha ngokuthe ngqo ibhengezwe zii-magnetic beads. Ngokusebenzisana kwentonga yemagnethi kunye nekama yencam yemagnethi, ukutsala umazibuthe, ukudlulisa, ukukhulula, ukuxuba kunye nezinye izinto ziyagqitywa ukwahlula ubuhlalu bemagnethi obuchaza i-acid ye-nucleic kwisisombululo se-lysis / sokubopha. Ukungcola okungafaniyo okungabotshelelwanga ngokuthe ngqo kumazibuthe ashukumayo kususwe kwindawo yokuhlamba, kwaye ekugqibeleni iimolekyuli ze-asidi ye-asidi ziyanyibilika kwindawo yokulumkisa.
Isixhobo sokuqhuba samkela isithuthi esinyukayo. Ngobukhulu obukhulu bokungcangcazela kwentsimbi yemagneti, i-amplitude ye-vibration ilungelelaniswa ngokuzenzekelayo ngokuhambelana nomthamo wesisombululo, iqinisekisa isiphumo sokuxuba esifanelekileyo. Isixhobo sokudlulisa sisebenzisa ibhola, kunye nokusebenza okuzinzileyo kwentonga yamagnetic, ukuchaneka okuphezulu kunye nobomi benkonzo ende. Zonke iindawo ezihambayo zixhotyiswe ngomda wokukhusela isikhundla ukuze kuqinisekiswe ukusebenza ngokucocekileyo nangocwangco kwesixhobo ukunqanda ukusilela.
Ngokusebenzisa imo yokukhangisa eyomeleleyo esandula ukuyilwa, ubuhlalu bemagneti bufakwe kwincam yentsimbi, ukuze phantsi kwemeko yevolumu encinci, i-eluent isenokugubungela onke amaso emagnethi. Ubunzima bemagneti bunefuthe elungileyo lokuthengisa kunye nokuveliswa okuphezulu kwe-acid ye-acid.
Ngokwesiseko sokusebenza kwamaqhosha okudala, ilawulwa kude kunye nokusebenza okuphezulu kweWindows Pad. Unxibelelwano lwenkqubo yesicelo yeWindows lusetelwe ngokugqibeleleyo, oluhambelana ngakumbi nomsebenzi oqhelekileyo wabathengi kunye nokufunda imikhwa yokusetyenziswa kweWindows.
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Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)
Ukukhutshelwa kwegazi le-Genomic DNA Isampulu: 200 μl frozen EDTA anticoagulant igazi liphela. IGenomic DNA inyityilikiswa kwi-100 μl Buffer TB. Umakishi weDNA: I-TIANGEN MD110, i-D15000 ye-DNA Marker |
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Ukukhutshwa kweNtsholongwane yeDNA / iRNA Isampulu: 200 μl frozen EDTA anticoagulant igazi liphela. IGenomic DNA inyityilikiswa kwi-100 μl Buffer TB. Umakishi weDNA: I-TIANGEN MD110, i-D15000 ye-DNA Marker |
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Izicubu zezilwanyana Ukukhutshwa kwe-DNA | |
Umlomo we-Swabs Genomic DNA Ukukhutshwa Isampulu: Isampuli yomlomo we-swab iyacinywa amaxesha angama-20 emlonyeni kwaye igcinwe kwityhubhu ye-centrifuge ene-450 μl Buffer GA. IGenomic DNA yachithwa kwi-60 μl Buffer TB. Umakishi weDNA: I-TIANGEN D15000 ye-DNA Marker |
Oko yasekwa, umzi-mveliso wethu uphuhlisa iimveliso zodidi lokuqala lwehlabathi ngokubambelela kumgaqo
esemgangathweni kuqala. Iimveliso zethu ziye zaziwa ngokugqwesileyo kushishino kunye nexabiso lokuthenjwa phakathi kwabathengi abatsha nabadala ..