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I-TIANprep Rapid Mini yePlasmid Kit

Ukucoca ngokukhawuleza kwe-plasmid DNA ye-molecular biology grade by alkaline lysis technique.

I-TIANprep Rapid Mini Plasmid Kit yenziwe ngcono kwitekhnoloji yendabuko ye-alkaline lysis, apho i-plasmid DNA ekumgangatho ophezulu inokucocwa ngaphakathi kwemizuzu esi-8. I-lysis buffer entsha ivumela ukubhengezwa kwe-DNA kwi-membrane yesilica phambi kwetyuwa ephezulu. Izinto ezenziwe ngayo inwebu ye-silica yahlukile, isebenza kakuhle kwaye icacise ngokukodwa. Le khithi yenzelwe ukucocwa ukuya kuthi ga kwi-24 μg plasmid DNA ukusuka kwi-1-4 ml ngobusuku beenkcubeko zeE.coli. I-plasmid DNA ehlanjululwe yile khithi inokusetyenziswa kuvavanyo olwahluka-hlukeneyo, olufana nokwetyiswa kwe-enzyme, i-PCR, ukulandelelana, ukudibanisa, ukutshintsha, ukuvavanywa kwethala leencwadi, ukukhutshelwa kwe-vitro kunye nokuhanjiswa kweeseli eziqhelekileyo zenkcubeko, njl.

Ikati. Hayi	Ubungakanani bokuPakisha
4992191	50 preps
4992192	200 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Ukuhamba komsebenzi

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

Ukukhawuleza: Inkqubo elula enamanyathelo amancinci okucoca iplasmid DNA emsulwa ngaphakathi kwemizuzu esi-8 kuphela.
■ Ukusebenza okuphezulu: Unokucoca ngaphezulu kwe-85% yeplasmid DNA kwi E.coli.
■ Isalathi sombala i-TIANRed sanikezelwa ukulawula nokukhumbuza yonke inkqubo ngexesha lokuzama ukuqinisekisa ukusebenza okuphezulu kokucocwa kweplasmid.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-TGuide S32 yeZithuthi zeNyukliya ezenzekelayo

Okulandelayo: Ikhithi ye-EndoFree Midi Plasmid

Ngonyango oluphezulu lwe-lysate kunye nenkqubo ye-buffer elungiselelwe, i-TIANprep Rapid Mini Plasmid Kit inokufumana ngokulula iimveliso ezisemgangathweni ngelixa igcina ixesha elininzi lokusebenza.

Thelekisa phakathi kwe-TIANprep Rapid Mini Plasmid Kit kunye noMthengisi A (ngokubanzi iplasmid DNA yokucoca ikiti) ngokucoca iplasmid efanayo ngaxeshanye.

Umthamo wesampula: 3 ml inkcubeko ngobusuku buka-E.coli (OD600 = 1.8); Ubungakanani bevolumu: 50 μl; Ukulayisha ivolumu: 1 μl pBS, 3 μl pET30a, 3 μl pBR322.

I-electrophoresis yaqhutywa nge-6 V / cm kwimizuzu engama-30 kwi-1% yejeli ye-agarose. M: ICandelo leDNA eliThengisiweyo IV.

Ukuqukumbela: I-Agarose gel electrophoresis ibonisa ukuba i-TIANprep Rapid Mini Plasmid Kit inokufumana imveliso efanayo ye-plasmid DNA njengoko injalo ngesixhobo sokucoca i-plasmid ngaphakathi kwe-8 min.

TIANprep Rapid Mini Plasmid Kit