I-TIANamp yeNtsholongwane yeRNA Kit

Ikhithi yokuhlanjululwa yintsholongwane yeRNA.

I-TIANamp Virus RNA Kit ibonelela ngendlela ekhawulezayo nefanelekileyo yokufumana ubunyulu be-RNA yentsholongwane. Isebenzisa itekhnoloji ye-silica membrane kunye ne-CarNA ye-RNA yokufunxa kunye nokukhupha uninzi lwe-virus ye-RNA kuhlobo lwesampulu. I-RNA yentsholongwane ihlanjululwe ngale khithi ikulungele ukusetyenziswa kwangoko ekufumanekeni okanye ezinye iinkqubo.

Ikati. hayi	Inombolo yee-preps
4992286	50 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo woVavanyo

Iimpawu zemveliso

Iimbonakalo

Isivuno esiphezulu: I-RNA yenethiwekhi inceda ekwandiseni imveliso yentsholongwane i-RNA.
Ukucoceka okuphezulu: Inwebu yeSilica ilahla ukungcola okuninzi.
Ukusetyenziswa okubanzi: Ifanelekile iintlobo ezahlukeneyo zeesampulu.
Umsebenzi okhawulezileyo: I-Virus RNA inokufunyanwa kwisithuba seyure enye.

Ukucaciswa

Uhlobo: Spin ikholamu esekwe
Isampuli: IPlasma, i-serum, ulwelo olungena selfowuni, njl.
Ithagethi: Intsholongwane RNA.
Ukuqala kwevolumu: ~ 140 μl
Ixesha lokusebenza: ~ 1 iyure
Izicelo ezisezantsi: I-RT-PCR / RT-qPCR, ukwakhiwa kwethala leencwadi le-NGS, iimpendulo ze-enzyme, njl.

Umbuzo: Incinci okanye ayikho i-DNA kwi-eluent.

A-1 Uxinzelelo olusezantsi lweeseli okanye intsholongwane kwisampulu yokuqala
-Tyebisa ukuxinana kweeseli okanye iintsholongwane.

A-2 I-lysis engonelanga yeesampulu
-Iisampulu azange zixutywe kakuhle kunye ne-lysis buffer. Kucetyiswa ukuba udibanise ngokucofa i-vortexing kangangezihlandlo ezi-1-2.
-I-lysis yeseli engonelanga ebangelwe kukuhla komsebenzi we-proteinase K.
-I-lysis engonelanga okanye ukuthotywa kweeprotheyini ngenxa yexesha lokuhlambela elifudumeleyo. Kucetyiswa ukuba usike izicwili zibe ziingceba kwaye wandise ixesha lokuhlamba ukususa yonke intsalela yelysate.

A-3 Ukungoneliseki kwe-DNA adsorption.
-Ayikho i-ethanol okanye ipesenti esezantsi endaweni ye-100% ye-ethanol eyongezwe ngaphambi kokuba kudluliselwe i-lysate kwikholamu yokujikeleza.

A-4 Ixabiso le-pH ye-elution buffer liphantsi kakhulu.
Lungisa i-pH iye phakathi kwe-8.0-8.3.

Q: I-DNA ayisebenzi kakuhle kulingelo lokuphendula kwe-enzymatic reaction.

Intsalela ye-ethanol kwi-eluent.
-Kukho intsalela yokuhlambela i-PW kwisikali. I-ethanol ingasuswa ngokufaka phakathi kwikholamu ejikelezayo ye-3-5 min, kwaye emva koko ubeke kubushushu begumbi okanye kwi-50 ℃ incubator ye-1-2 min.

Q: Ukonakaliswa kweDNA

A-1 Isampulu ayisiyonto intsha.
-Khupha isampulu yeDNA elungileyo njengokulawula ukumisela ukuba ingaba iDNA ekwisampulu ihlaziyiwe.

Unyango lwangaphambi kokunyanga okungalunganga.
-Yenziwe kukugaya i-nitrogen engamanzi ngokugqithisileyo, ukubuyisa ukufuma, okanye isixa esikhulu sesampulu.

Umbuzo: Uyenza njani inyanzelo yokukhutshwa kwe-gDNA?

Ukufakwa kwangaphambili kufuneka kwahluka kwiisampulu ezahlukeneyo. Kwiisampulu zezityalo, qiniseka ukuba usile kakuhle kwi-nitrogen engamanzi. Kwiisampulu zezilwanyana, homogenate okanye ugaye kakuhle kwi-nitrogen engamanzi. Kwiisampulu ezineendonga zeseli ekunzima ukuziqhekeza, ezinjenge-G + bacteria kunye nemvubelo, kucetyiswa ukuba kusetyenziswe i-lysozyme, i-lyticase okanye iindlela zoomatshini zokwaphula iindonga zeseli.

Q: Nguwuphi umahluko phakathi kweekiti ezintathu zokukhupha i-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 ye-Genomic DNA Kit isebenzisa indlela esekwe kwikholamu efuna ikloroform yokukhupha. Ifanelekile ngakumbi kwiisampulu ezahlukeneyo zezityalo, kunye nomgubo owomileyo wezityalo. I-Hi-DNAsecure Plant Kit nayo isekwe kwikholamu, kodwa kungekho mfuneko yokukhutshwa kwe-phenol / chloroform, ukuyenza ikhuseleke kwaye ingabinetyhefu. Kufanelekile ukuba izityalo ezine-polysaccharides ephezulu kunye nomxholo we-polyphenol. 4992709/4992710 DNAquick Plant System yamkela indlela esekwe kulwelo. Ukukhutshwa kwePhenol / chloroform akufuneki nako. Inkqubo yokuhlanjululwa ilula kwaye iyakhawuleza ngaphandle komda kwiisampulu zezixa zokuqala, ke abasebenzisi banokuhlengahlengisa isixa ngokuhambelana neemfuno zovavanyo. Ubungakanani obukhulu bamaqhekeza e-gDNA anokufunyanwa ngesivuno esikhulu.

Q: Sesiphi isivuno esiqikelelweyo se-gDNA kwi-1 ml yesampulu yegazi yi-TIANamp Blood DNA Kit?

I-genomic DNA yakhutshwa kwimilinganiselo eyahlukeneyo yeesampulu zabantu zegazi yi-TIANamp Blood DNA Kit. Iziphumo zilandelayo. Iziphumo zidweliswe njengereferensi kuphela, ezona ziphumo zokukhupha zixhomekeke kwimeko yeesampulu.

Q: Ngaba i-4992207/4992208 kunye ne-4992722/4992723 ingasetyenziselwa ukukhupha i-DNA yegazi?

Ukukhutshelwa kwegazi le-DNA kungena kusetyenziswa ii-reagents ezibonelelwe kwezi khithi zimbini ngokutshintsha nje umthetho olandelwayo kumyalelo othile wokukhutshwa kwegazi le-DNA. Ikopi ethambileyo yenkqubo yokutsalwa kwegazi kwi-DNA inokukhutshwa xa uceliwe.

Q: Xa ufaka isicelo se-TIANamp Genomic DNA Kit, ungaqhekeza njani izicwili ezitsha ekumisweni kweseli?

Misa isampulu entsha nge-1 ml ye-PBS, isaline eqhelekileyo okanye i-TE buffer. Ngokupheleleyo homogenize isampuli yi homogenizer kwaye uqokelele isitshisi ukuya ezantsi kumbhobho nge-centrifuging. Yilahle into engaphaya kwamandla, kwaye uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde wenziwe khona nge-200 μl buffer GA. Ukucocwa kwe-DNA elandelayo kunokwenziwa ngokomyalelo.

Q: Uyikhetha njani imveliso yokukhutshwa kwe-DNA kwiplasma, i-serum kunye neesampulu zamanzi omzimba?

Ukucoca i-gDNA kwi-plasma, i-serum kunye neesampuli zomzimba, kuyacetyiswa i-TIANamp Micro DNA Kit. Ukucoca intsholongwane gDNA kwiisampulu ze-serum / plasma, I-TIANamp Virus DNA / RNA Kit iyacetyiswa. Ukucoca i-gDNA yebhaktiriya kwiisampulu ze-serum kunye neplasma, i-TIANamp Bacteria DNA Kit iyacetyiswa (i-lysozyme kufuneka ibandakanywe kubhaktiriya). Kwiisampulu zamathe, iHi-Swab DNA Kit kunye ne-TIANamp Bacteria DNA Kit ziyacetyiswa.

Q: Ungazikhetha njani iikiti ze-gDNA zokukhupha kwiisampulu zomngundo?

I-DNAsecure Plant Kit okanye i-DNAquick Plant System iyacetyiswa ukuba isuswe kwi-fungal genome extraction. Ukukhutshwa kwegwele le-genome, i-TIANamp Yeast DNA Kit iyacetyiswa (i-lyticase kufuneka izilungiselele).

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo Ukuveliswa kwe-Rna ye-Original Factory - I-TGuide iiseli / iiTissue / izityalo zeRNA Kit - TIANGEN

Okulandelayo: I-TIANSeq Ikiti yeLayibrari ekhawulezayo yeDNA (ukukhanya)

I-AIV-H5 yahlanjululwa yaya kwi-10^-5-10^-8Iigradients ngamanzi e-Mill-Q. Isampulu ye-140 μl yakhutshwa yi-TIANamp Virus RNA Kit (DP315-R) kunye nekhithi efanelekileyo kuMthengisi A. Xa kuthelekiswa noMthengisi A, iTIANGEN inentsebenzo ephezulu yesampulu yoxinaniso esezantsi.
I-RT-qPCR ikhithi yokufumanisa: I-TIANGEN, i-FastKing inyathelo elinye i-RT-qPCR kit (Probe)
Isixhobo se-RT-qPCR: Inkqubo ye-PCR ye-AB 7500 ekhawulezayo.