Ikhithi ye-EndoFree Midi Plasmid

Ukucocwa ngokukhawuleza kwexabiso eliphakathi kwe-endotoxin-free transfection grade plasmid DNA.

I-EndoFree Midi Plasmid Kit isebenzisa ubuchule obukhethekileyo be-silica membrane adsorption ngokufanelekileyo nangokukhethekileyo ibopha i-plasmid DNA. I-Buffer EBT ekhethekileyo kunye neFiltration CS1 zinokususa ngokufanelekileyo ukungcola okunje nge-endotoxin kunye neprotein. Inkqubo yonke yokukhupha ithatha iyure enye, efanelekileyo kwaye ekhawulezayo.

Ikati. Hayi	Ubungakanani bokuPakisha
4992853	10 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Ukuhamba komsebenzi

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

■ Kukulungele ukufunxa iiplasmids kwi-50-100 ml yeebhaktiriya zenkcubeko.
■ Iplasmid ehlanjululweyo iqulethe i-endotoxin esezantsi kakhulu kwaye iyakwazi ukuhlangabezana neemfuno zokuhanjiswa okumgangatho ophezulu.
■ Kulula ukusebenza ngendlela ekhethekileyo nesheshayo yokususa i-endotoxin.

Izicelo

I-plasmid DNA ehlanjululweyo inokusetyenziswa kuvavanyo lwebhayoloji kunye nokuvavanywa kweseli yebhayoloji njengokuthintela ukwetyisa ukutya, ukulandelelana, inguqu, ukuhanjiswa, into encinci, njl.

Ukuveliswa kwePlasmid

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-TIANprep Rapid Mini yePlasmid Kit

Okulandelayo: I-TIANprep Rapid N96 Plasmid Kit

	Iplasmid i-PEGFP-C1 ihlanjululwe yi-EndoFree Midi Plasmid Kit ukusuka kwi-50 ml ngobusuku obuphakathi kwintsholongwane. Uxinzelelo lweplasmid lwalulinganiswa ngeNanodrop kwaye isivuno sabalwa. Iziphumo zibonisa ukuba imveliso yeplasmid eyenziwe yi-EndoFree Mini Plasmid Kit iphezulu kunaleyo yimveliso efanelekileyo evela kuMthengisi A.
	Iplasmid pEGFP-C1 ekhutshwe yi-EndoFree Midi Plasmid Kit idluliselwe kwi-80% yokuhlangana kweeseli ezingama-293T yi-TIANGEN TIANfect. Inkcazo ye-GFP yafunyanwa nge-24-36 h emva kokudluliselwa. Iziphumo zibonisa ukuba ukusebenza ngokugqibeleleyo yayingu-80% -90%.