I-TIANamp yegazi yeDNA Kit

Ukucoca i-genomic DNA yegazi.

I-TIANamp yegazi le-DNA Kit isebenzisa ikholamu ejikelezayo enokuthi ibophe ngokuthe ngqo i-DNA kwaye ibonelele ngenkqubo ekhethekileyo ye-buffer yokukhutshwa kwegazi kwe-gDNA. I-Genomic DNA ebekelwe bucala kunye nale khithi inokucoceka okuphezulu, uzinzo kunye nokuthembeka. I-DNA ehlanjululweyo inokusebenza ngokuthe ngqo njengeetemplate zokufumanisa ubukho bezantsi okanye ezinye iinkqubo.

Ikati. Hayi	Ubungakanani bokuPakisha
4992207	50 preps
4992208	200 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

FAQ

Iimpawu zemveliso

Iimbonakalo

■ Ukucoceka okuphezulu: I-Silica membrane isusa konke ukungcola.
■ Ukusetyenziswa okubanzi: Ilungele igazi le-anticoagulant (EDTA, heparin njl.
■ Ukusebenza okukhawulezileyo: IGenomic DNA inokufumaneka kwisithuba seyure enye.

Ukucaciswa

Uhlobo: Spin ikholamu esekwe
Isampulu kunye nevolumu yokuqala: 100-1000 μl igazi elipheleleyo elivela kwizilwanyana ezanyisayo, i-5-20 μl igazi elipheleleyo kwintaka okanye kwi-amphibian, 200-1000 μl yegazi
Ithagethi: IGenomic DNA.
Isivuno: 3-40 μg
Ixesha lokusebenza: ~ 1 iyure
Izicelo ezisezantsi: Ukuthintela ukwetyisa i-enzyme, uhlalutyo lwe-PCR, iblot eseMazantsi, i-chip hybridization, ulwakhiwo lwethala le-NGS, njl.

Amanqaku abalulekileyo

1. Ukususa iintsalela zeRNA, isisombululo seRNase A (100 mg / ml) kufuneka sizilungiselele.
2. Le mveliso ibhalisiwe njengodidi I i-In vitroI-reagent yokuqonda isifo yi-Beijing FDA. Nceda unxibelelane neTIANGEN ukuba ulwazi oluthe kratya luyafuneka.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo Amagqabantshintshi egazi Magnetic Kit

Okulandelayo: RelaxGene Inkqubo yeDNA yeGazi (0.1-20ml)

I-Genomic DNA yakhutshwa kwiisampulu zegazi kusetyenziswa i-TIANamp yegazi leDNA Kit.
M: Isiphawuli seTIANGEN D15000.
Iisampulu: i-200 μl yegazi le-anticoagulation yegazi ene-EDTA, i-EDTA / NaF, i-heparin kunye ne-sodium citrate; ibhatyi e-2 ml yegazi; I-10 μl yegazi yenkukhu kunye ne-200 μl yegazi legazi. Kwibhatyi ye-buffy, i-2 μl ye-100 μl eluates yalayishwa; kwabanye, i-4 μl ye-100 μl eluates yalayishwa kwindlela nganye.
Iziphumo zovavanyo: I-TIANamp Igazi le-DNA Kit linokusetyenziswa ngokubanzi kumgangatho wokucocwa kwe-DNA kwizithambisi zabantu ezinonyango olwahlukeneyo kunye negazi leentlobo ezahlukeneyo.

Thelekisa i-genomic DNA ekhutshwe kwi-200 μl ye-anticoagulation yabantu kunye ne-EDTA isebenzisa i-TIANamp yegazi le-DNA Kit kunye neemveliso ezifanayo kwabanye abathengisi. M: Isiphawuli seTIANGEN D15000. I-4 μl ye-100 μl eluates ilayishwe ngomgaqo ngamnye.
Iziphumo zovavanyo: Thelekisa nabanye abathengisi, I-TIANamp Igazi le-DNA Kit inokufumana isivuno esiphezulu segazi le-gDNA.

Umbuzo: Incinci okanye ayikho i-DNA kwi-eluent.

A-1 Ukuxinana kweeseli okanye intsholongwane kwisampulu yokuqala -Tyebisa uxinzelelo lweeseli okanye iintsholongwane.

A-2 I-lysis engonelanga yeesampulu -Iisampulu azange zixutywe kakuhle kunye ne-lysis buffer. Kucetyiswa ukuba udibanise ngokucofa i-vortexing kangangezihlandlo ezi-1-2. -I-lysis yeseli engonelanga ebangelwe kukuncipha kwemisebenzi ye-proteinase K. Kucetyiswa ukuba usike izicwili zibe ziingceba kwaye wandise ixesha lokuhlamba ukususa yonke intsalela yelysate.

A-3 Ukungoneliseki kwe-DNA adsorption. -Ayikho i-ethanol okanye ipesenti esezantsi endaweni ye-100% ye-ethanol eyongezwe ngaphambi kokuba kudluliselwe i-lysate kwikholamu yokujikeleza.

A-4 Ixabiso le-pH ye-elution buffer liphantsi kakhulu. Lungisa i-pH iye phakathi kwe-8.0-8.3.

Q: I-DNA ayisebenzi kakuhle kulingelo lokuphendula kwe-enzymatic reaction.

Intsalela ye-ethanol kwi-eluent.

-Kukho intsalela yokuhlambela i-PW kwisikali. I-ethanol ingasuswa ngokufaka phakathi kwikholamu ejikelezayo ye-3-5 min, kwaye emva koko ubeke kubushushu begumbi okanye kwi-50 ℃ incubator ye-1-2 min.

Q: Ukonakaliswa kweDNA

A-1 Isampulu ayisiyonto intsha. -Khupha isampulu yeDNA elungileyo njengokulawula ukumisela ukuba ingaba iDNA ekwisampulu ihlaziyiwe.

Unyango lwangaphambi kokunyanga okungalunganga. -Yenziwe kukugaya i-nitrogen engamanzi ngokugqithisileyo, ukubuyisa ukufuma, okanye isixa esikhulu sesampulu.

Umbuzo: Uyenza njani inyanzelo yokukhutshwa kwe-gDNA?

Ukufakwa kwangaphambili kufuneka kwahluka kwiisampulu ezahlukeneyo. Kwiisampulu zezityalo, qiniseka ukuba usile kakuhle kwi-nitrogen engamanzi. Kwiisampulu zezilwanyana, homogenate okanye ugaye kakuhle kwi-nitrogen engamanzi. Kwiisampulu ezineendonga zeseli ekunzima ukuziqhekeza, ezinjenge-G + bacteria kunye nemvubelo, kucetyiswa ukuba kusetyenziswe i-lysozyme, i-lyticase okanye iindlela zoomatshini zokwaphula iindonga zeseli.

Q: Nguwuphi umahluko phakathi kweekiti ezintathu zokukhupha i-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 ye-Genomic DNA Kit isebenzisa indlela esekwe kwikholamu efuna ikloroform yokukhupha. Ifanelekile ngakumbi kwiisampulu ezahlukeneyo zezityalo, kunye nomgubo owomileyo wezityalo. I-Hi-DNAsecure Plant Kit nayo isekwe kwikholamu, kodwa kungekho mfuneko yokukhutshwa kwe-phenol / chloroform, ukuyenza ikhuseleke kwaye ingabinetyhefu. Kufanelekile ukuba izityalo ezine-polysaccharides ephezulu kunye nomxholo we-polyphenol. 4992709/4992710 DNAquick Plant System yamkela indlela esekwe kulwelo. Ukukhutshwa kwePhenol / chloroform akufuneki nako. Inkqubo yokuhlanjululwa ilula kwaye iyakhawuleza ngaphandle komda kwiisampulu zezixa zokuqala, ke abasebenzisi banokuhlengahlengisa isixa ngokuhambelana neemfuno zovavanyo. Ubungakanani obukhulu bamaqhekeza e-gDNA anokufunyanwa ngesivuno esikhulu.

Sesiphi isivuno esiqikelelweyo se-gDNA kwi-1 ml yesampulu yegazi yi-TIANamp Blood DNA Kit?

I-genomic DNA yakhutshwa kwimilinganiselo eyahlukeneyo yeesampulu zabantu zegazi yi-TIANamp Blood DNA Kit. Iziphumo zilandelayo. Iziphumo zidweliswe njengereferensi kuphela, ezona ziphumo zokukhupha zixhomekeke kwimeko yeesampulu.

Q: Ngaba i-4992207/4992208 kunye ne-4992722/4992723 ingasetyenziselwa ukukhupha i-DNA yegazi?

Ukukhutshelwa kwegazi le-DNA kungena kusetyenziswa ii-reagents ezibonelelwe kwezi khithi zimbini ngokutshintsha nje umthetho olandelwayo kumyalelo othile wokukhutshwa kwegazi le-DNA. Ikopi ethambileyo yenkqubo yokutsalwa kwegazi kwi-DNA inokukhutshwa xa uceliwe.

Q: Xa ufaka isicelo se-TIANamp Genomic DNA Kit, ungaqhekeza njani izicwili ezitsha ekumisweni kweseli?

Misa isampulu entsha nge-1 ml ye-PBS, isaline eqhelekileyo okanye i-TE buffer. Ngokupheleleyo homogenize isampuli yi homogenizer kwaye uqokelele isitshisi ukuya ezantsi kumbhobho nge-centrifuging. Yilahle into engaphaya kwamandla, kwaye uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde wenziwe khona nge-200 μl buffer GA. Ukucocwa kwe-DNA elandelayo kunokwenziwa ngokomyalelo.

Q: Uyikhetha njani imveliso yokukhutshwa kwe-DNA kwiplasma, i-serum kunye neesampulu zamanzi omzimba?

Ukucoca i-gDNA kwi-plasma, i-serum kunye neesampuli zomzimba, kuyacetyiswa i-TIANamp Micro DNA Kit. Ukucoca intsholongwane gDNA kwiisampulu ze-serum / plasma, I-TIANamp Virus DNA / RNA Kit iyacetyiswa. Ukucoca i-gDNA yebhaktiriya kwiisampulu ze-serum kunye neplasma, i-TIANamp Bacteria DNA Kit iyacetyiswa (i-lysozyme kufuneka ibandakanywe kubhaktiriya). Kwiisampulu zamathe, iHi-Swab DNA Kit kunye ne-TIANamp Bacteria DNA Kit ziyacetyiswa.

Q: Ungazikhetha njani iikiti ze-gDNA zokukhupha kwiisampulu zomngundo?

I-DNAsecure Plant Kit okanye i-DNAquick Plant System iyacetyiswa ukuba isuswe kwi-fungal genome extraction. Ukukhutshwa kwegwele le-genome, i-TIANamp Yeast DNA Kit iyacetyiswa (i-lyticase kufuneka izilungiselele).

Iselfowuni eDna yokuSusa iKhithi