■ Okukhawulezayo: I-Antibody eguqulweyo ye-Anti Taq DNA polymerase, isebenzisana ne-Buffer system ekhethekileyo, isindisa ukuya kuthi ga kwi-60% yexesha lokuphendula kwaye ibe yeyona SYBR iluhlaza ngokukhawuleza kwimarike okwangoku.
■ Amandla okomeleza amandla: Umqondiso wokukhulisa ukukhanya kwe-fluorescence, iziphumo ezichanekileyo nezithembekileyo.
■ uzinzo olulungileyo: Isiqinisi sePCR esisodwa kunye nesomelezi songezwa kwiBuffer, senza ukuba iziphumo zizinze, ziphindaphindwe, zichaneke kwaye zithembeke.
■ Ukusebenza ngokubanzi: Ayilungelanga kuphela ixesha elikhawulezileyo lezixhobo zePCR, kodwa ikwalungele nezixhobo eziqhelekileyo zePCR.
■ Ukulungiswa kwe-ROX: Idayi ye-ROX ipakishwe ngokwahlukeneyo, eguqukayo ngakumbi ukuyisebenzisa kwaye inokuqinisekisa iziphumo ezichanekileyo.
Uhlobo: I-DNA polymerase yokuqalisa ngokutsha komzimba, i-SYBR Green I
Ixesha lokusebenza: ~ 30 min
Izicelo: I-qPCR yedayi esekwe kwidayi yokufumanisa ubunjani kwi-DNA okanye uhlalutyo lwentetho kwi-cDNA yeentlobo ezahlukeneyo
Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)
Amandla okukhulisa amandla- umqondiso we-fluorescence eyomeleleyo Ukukhawuleza-ukuphucula ukusebenza kokulinga, ukwandisa ubomi benkonzo yesixhobo kunye nokonga amandla Taq I-DNA polymerase ingenziwa isebenze ngokukhawuleza, iqinisekisa ukuba iimveliso ze-FastFire ziyeyona nto ikhawulezayo kwimarike okwangoku kwintengiso yangoku ngenkqubo yeBuffer ekhawulezileyo yokuphucula ukusebenza kovavanyo. |
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Amandla okukhulisa amandla- umqondiso we-fluorescence eyomeleleyo Isiginali ye-fluorescence eyandisiweyo yomelele (ukukhulisa amandla kunamandla), kunye nomjikelo wokukhulisa umgangatho ngakumbi, ubuntununtunu obuphezulu, kwaye ngokuchanekileyo nangokubala ubuninzi bokubona uhlobo ekujolise kulo itemplate esezantsi. Umqondiso wokufumanisa wemveliso efanelekileyo kuMthengisi T ubuthathaka, kwaye ixabiso le-CT libuyela umva, elinokukhokelela kwiziphumo ezingachanekanga. |
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Uvavanyo olu-96 oluhambelana ngokufanayo lubonisa ukuba i-reagent inozinzo olomeleleyo kunye nokuphindeka okungcono. |
Oko yasekwa, umzi-mveliso wethu uphuhlisa iimveliso zodidi lokuqala lwehlabathi ngokubambelela kumgaqo
esemgangathweni kuqala. Iimveliso zethu ziye zaziwa ngokugqwesileyo kushishino kunye nexabiso lokuthenjwa phakathi kwabathengi abatsha nabadala ..