Talent qPCR PreMix (SYBR Green) Featured Image

Italente qPCR PreMix (SYBR Green)

Ukumelana kakuhle nokuphazamiseka kokungacoceki kunye nokulinganisa ngokukhawuleza kweetemplate ezimbaxa.

Le mveliso yinto ebonakalayo ekhethekileyo ye-Real-Time PCR isebenzisa i-SYBR Green I chimeric fluorescence. I-2 × yeTalente qPCR PreMix yamkela i-anti-Taq DNA polymerase ye-anti-anti, kunye nenkqubo ye-qPCR Buffer eyahlukileyo inokuqinisekisa ukuba imveliso iyenza ngokukhawuleza kwaye iyakhawuleza ukusabela kwe-qPCR kuzo zonke izixhobo ze-Real-Time PCR. Ixesha lokuphendula le-qPCR linokucuthwa ngama-50%. Ukongeza, ukongezwa kwento eyi-H-Competitor factor kunye ne-EP kwiBuffer kwenza ukuba imveliso ibe noluhlu olubanzi lwesampulu kwindalo iphela. Inokusebenziseka okuhle kakhulu kwiitemplate ezinezakhiwo ezintsonkothileyo eziphambili, iitemplate ezinentsalela engaphezulu ye-PCR inhibitor, ukukhulisa iziqwenga ezinde, njl.
Imveliso ikwanazo neempawu zokukhulisa okuphezulu, ukucaciswa okuphezulu, uluhlu oluthembekileyo, njl njl, kwaye unokufumana iziphumo ngokukhawuleza ngaphandle kokuchaphazela ukusebenza kwePCR.

Ikati. Hayi	Ubungakanani bokuPakisha
4992882	20 ×l × 125 rxn
4992775	20 ×l × 500 rxn
4992866	20 ×l × 5000 rxn

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

Modified I-antibody e-ethe-ethe yolungiso lwe-polymerase inokulinciphisa ixesha lokuphendula ngesiqingatha.
■ Umqhubi we-H ukhuphisana ne-hydrogen bond, eqinisekisa ukusebenza ngokunyusa okuphezulu kweetemplate ezinomxholo ophezulu we-GC, ulwakhiwo lwesekondari oluntsonkothileyo kunye neetemplate ezinde.
Component Icandelo le-EP lizinzisa inkqubo ye-PCR, ikhusela ngokufanelekileyo umsebenzi we-enzyme kwaye iyala ukuphazamiseka kwezithinteli ezahlukeneyo.
■ Ityhubhu ebonakalayo engenambala ithatha indawo yetyhubhu emdaka, ukuze isixa esishiyekileyo sibonakale.

Izicelo

Kufanelekile ukuba kwenziwe uhlalutyo lwentetho, ukubonwa kwe-acid ye-acid kunye nezinye iintlobo zovavanyo kwizixhobo ezahlukeneyo zexesha le-PCR ngendlela ye-SYBR Green, ngakumbi kwi-GC ephezulu, ulwakhiwo lwesekondari oluntsonkothileyo, intsalela yokungcola okuphezulu kunye nokulinganisa kwetemplate ende.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-FastFire qPCR PreMix (Probe)

Okulandelayo: Ikhithi yoHlolo lwe-HRM (i-EvaGreen)

Experimental Example

Uhlobo lomntu lwe-UH11 (GC: 73.4%) lwachongwa yi-TIANGEN Talent qPCR PreMix (ekhohlo) kunye nemveliso efanelekileyo evela kuMboneleli A (phakathi) kunye no-B (ngasekunene). Iziphumo zibonisa ukuba xa kuthelekiswa nabanye abathengisi, i-Talent qPCR PreMix inee-curves ezicacileyo ze-5 gradients, ixabiso eliphantsi le-Ct, ixabiso eliphezulu le-fluorescence, kunye ne-curve eyodwa yokunyibilika, ebonisa ukuba i-Talent qPCR PreMix inokulungelelanisa okuhle kunye nokusebenza ngokunyusa kwiitemplate eziphezulu ze-GC.

Bhala umyalezo wakho apha uze uyithumele kuthi