SuperReal PreMix Color (SYBR Green) Featured Image

Umbala weSuperReal PreMix (SYBR Green)

Ukuqwalaselwa kunye nedayi ethile ye-fluorescence reagentative reagent.

Imveliso yinto ekhethekileyo ye-reagent ye-PCR yexesha lokwenyani isebenzisa i-SYBR Green I chimeric fluorescence. Imveliso yi-2 × yoxinaniso lwe-PreMix exutywe kunye noxinzelelo oluphezulu lwe-SYBR Green endiyifunayo ekuphenduleni kwe-PCR yokwenyani. Isalathi sidityaniswe songezwa kwimveliso, esiluncedo ekulayisheni inani elikhulu leesampulu kwaye sinciphise amathuba okungahambi kakuhle.

I-SuperReal Colour PreMix yamkela into eyahlukileyo enesicatshulwa sokuqala esishushu seDNA polymerase (HotStart eguqulweyo ngemichiza Taq I-DNA polymerase kunye ne-anti-anti anti eguqulweyo Taq I-DNA polymerase), kwaye ibonakaliswa ngokusebenza okuphezulu kokukhulisa, ukukhulisa okuphezulu kunye noluhlu oluthembekileyo ngokudibeneyo kunye nenkqubo ye-buffer elungiselelwe ngononophelo. I-reagent ephuculweyo inezibonelelo ezibalaseleyo ngakumbi kwisikhululo esithile emva kohlengahlengiso lwecandelo.

Ikati. Hayi	Ubungakanani bokuPakisha
4992777	20 ×l × 125 rxn
4992876	20 ×l × 500 rxn
4992902	20 ×l × 5000 rxn

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

Indicators Iimpawu zombala zongezwa kwizenzi ukuze kuthintelwe iimpazamo zokongezwa kwesampulu okanye ukushiywa ngenxa yenani elikhulu leesampulu.
■ Ixabiso eliphezulu le-fluorescence, uvakalelo oluphezulu kunye nokucaciswa okuphezulu.
After Emva kokubhaqwa ngokungqongqo, isalathi asizukuchaphazela ixabiso le-fluorescence kunye neziphumo zobungakanani bedayi.
■ Zilungele izixhobo ezahlukeneyo zexesha lokwenyani le-PCR (iRoche, iBio-rad, iABI, njl.njl.).

SuperReal PreMix Color (SYBR Green）

Izicelo

Ilungele iintlobo ezahlukeneyo zovavanyo ezinje ngohlalutyo lokubonisa kunye nokuchongwa kwe-acid yeicicic yindlela ye-SYBR Green kwizixhobo ezahlukeneyo zexesha lePCR. Ilungele ngokukodwa ukuvavanywa okuphezulu okuphuma kunye nenani elikhulu leesampulu kunye nokusebenza kokulayishwa kwesampulu okunzima.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-TIAN nangona kunjalo i-genotyping qPCR PreMix (Probe)

Okulandelayo: Umbala weSuperReal PreMix (Probe)

Umzobo. 1. Iigrafu ze-amplifi cation curves kunye nokunyibilika kwee-curve ezifunyenwe ngokulandelelana kokulinganisa uhlobo lomntu lwe-HS1 usebenzisa i-TIANGEN SuperReal PreMix Plus (SYBR Green) (A) kunye noMbala weSuperReal PreMix (SYBR Green) (B). Ithemplate ihlanjululwe kwi-5 gradients. Amaxabiso e-Ct (C) afunyenwe ngokuthelekisa abonisa ukuba ukuphakanyiswa kweekiti ezimbini kunobuntununtunu obuninzi, ukubaluleka kunye nokuzinza. Uvavanyo lwaluqhutywa eRoche LightCycler480.

Umzobo 2. Iigrafu zokukhulisa ijika kunye nokunyibilika kwegophe kufunyenwe ngokulandelelana kokulinganisa uhlobo lomntu lwe-HS5 usebenzisa i-TIANGEN SuperReal PreMix Colour (SYBR Green) kunye nemveliso efanelekileyo evela kuMthengisi T (A: TIANGEN, B: Umthengisi T. Nciphisa itemplate yeegradients zoxinzelelo ezi-5, Thelekisa amaxabiso e-Ct (C) .Isiphumo sibonisa ukuba umbala weSuperReal PreMix (SYBR Green) unobuntununtunu obuphezulu, ukucaciswa kunye nozinzo.Uvavanyo lwaluqhutywa kwi-Bio-Rad CFX96.

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