TIANtough Genotyping qPCR PreMix (Probe) Featured Image

I-TIAN nangona kunjalo i-genotyping qPCR PreMix (Probe)

Probe reagent yokuchwetheza ngokuchanekileyo indawo yeSNP.

I-TIANexact Genotyping qPCR PreMix (Probe) ilungele ukusebenzisa i-2 × eshushu-ukuqala kwePCR yeprimix reagent esetyenziswe ngokukodwa kuphando-isekwe Ukufunyanwa kwe-SNP. I-reagent esele ibekiwe iqulethe i-antibody ethile eguqulweyoTaq I-DNA polymerase kwaye inokubona ngokufanelekileyo ubuninzi beetemplate zeDNA eziphantsi kakhulu. Ukulungiswa ngokukodwa kwe-PCR ye-buffer inokuphelisa ngokufanelekileyo ukuthintela okuninzi kwi-PCR kunye nefuthe lokucinywa komqondiso we-fluorescence. Le khithi inolwazi oluchanekileyo kunye nokusebenza ngokunyusa isazisi se-SNP, ngakumbi iisampulu ezingafunekiyo, ezibonisa uxinzelelo oluphezulu.

Ikati. Hayi	Ubungakanani bokuPakisha
4992874	20 ×l × 125 rxn
4992875	20 ×l × 500 rxn

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

■ Ukucaciswa okuphezulu: Isixhobo esikhethekileyo sokuchasana nesitshabalalisi esitshisiweyo se-Taq DNA polymerase sinciphisa ukukhulisa okungangqalanga kunye nokuphucula ukubalula.
■ Ukufakwa kwe-genotyping esebenzayo: Inkqubo ye-Buffer eyodwa inokuyikhusela ngokufanelekileyo ifuthe le-PCR inhibitors ku-SNP typing.
■ Ukuchaneka okuphezulu: I-SNP yokuchwetheza imiqondiso ye-fluorescence yenza ukuba kube lula ukufumana iziphumo.
■ Ukusetyenziswa okubanzi: Kusetyenziswa ukutyala, izilwanyana kunye nezinye iisampulu, zikwasebenza ekufumaneni isicatshulwa esingafunekiyo sezinye iisampulu zokusingqongileyo.
■ Isalathiso esiluhlaza: Isimangalo esenziwe kwangaphambili sinedayi yesalathiso esiluhlaza ukunqanda isampulu yokulayisha iimpazamo.
■ Ukulungiswa kwe-ROX: Idayi ye-ROX ipakishwe yodwa, eguqukayo ngakumbi ukuyisebenzisa kwaye ineziphumo ezichanekileyo.

Ukucaciswa

Uhlobo: I-antibody esekwe kwi-antibody e-Taq DNA polymerase
Izicelo: Ukuchwetheza kwe-SNP eyaziwayo

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo Ikhithi yoHlolo lwe-HRM (i-EvaGreen)

Okulandelayo: Umbala weSuperReal PreMix (SYBR Green)

	Xa kuthelekiswa noMncintiswano I, i-TIANexact ibonisa isignali ye-fluorescence enamandla kunye nefuthe lokudibanisa ngokuqinileyo phantsi kwemeko yetemplate yoxinaniso esezantsi.
	Xa unyangwa nge-humic acid (50 ng / μl), i-PCR yokukhulisa i-Competitor I ithintelwe ngokupheleleyo, ngelixa i-TIANexact isabonisa iziphumo ezichanekileyo.