Izicubu zeMagnetic / Iseli / iGazi lilonke kwiRNA Kit

Ukukhupha i-RNA kwiisampulu ezahlukeneyo ezinje ngegazi leeseli zethishu enegalelo eliphezulu.

I-Magnetic Tissue / Cell / Blood Iyonke i-RNA Kit yamkela ubuhlalu bemagnethi ngomsebenzi owahlukileyo wokwahlula kunye nenkqubo ye-buffer eyahlukileyo yokwahlula nokucoca i-RNA ekumgangatho ophezulu. Imveliso inokutshatiswa ngokugqibeleleyo neKingfisher Flex96 kunye ne-TGuide S32 / S96 eyi-Automated Nucleic Acid Extractors. Ukuba ukuveliswa okuphezulu okuzenzekelayo kuyafuneka, nceda unxibelelane neTIANGEN yesisombululo sokudibanisa.

Ikati. Hayi	Ubungakanani bokuPakisha
4992740	50 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Imizekelo yovavanyo

FAQ

Iimpawu zemveliso

Iimbonakalo

■ Kulula kwaye kuyakhawuleza: I-RNA iyonke ye-Ultrapure inokufunyanwa kwisithuba semizuzu engama-60.
■ Ukuhamba okuphezulu: Inokuhlangabezana neemfuno zokukhupha ngesandla kunye nebhetshi yokukhutshwa kumaqonga ahlukeneyo aphezulu.
■ Ukhuselekile kwaye awuyityhefu: Akukho reagent enjenge phenol / chloroform efunekayo.

Ukucaciswa

Uhlobo: Uhlobo lweentsimbi Magnetic uhlobo utsalo.
Isampuli: Izicubu, iiseli kunye negazi.
Ithagethi: Iyonke iRNA
Ukuqala kwevolumu: 5-20 mg, ingagqithi kwi-1 × 10⁷, 0.1-1.5 ml
Ixesha lokusebenza: 60 min
Izicelo ezisezantsi: I-RT-PCR / RT-qPCR, ulwakhiwo lwethala leencwadi le-NGS, njl.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo Ukujikeleza kweMagnetic Max Max Kit V2

Okulandelayo: I-TGuide S96 Magnetic Universal Kit Kit

Iyonke i-RNA ikhutshwe kwisibindi se-20 mg ye-rat ngesibindi kunye ne-TIANGEN Magnetic Tissue / Cell / Blood Iyonke i-RNA Kit kunye neemveliso ezifanelekileyo ezivela kwabanye abathengisi. I-5 μl ye-200 μl eluate yafakwa kwi-1% ye-agarose gel electrophoresis, 6 V / cm.
Isiphelo: Isivuno sokukhupha, ubunyulu kunye nozinzo lweTIANGEN Magnetic Tissue / Cell / Blood Iyonke iRNA Kit ingcono kunaleyo yabanye ababoneleli.

Iyonke i-RNA yakhutshwa kwi-20 mg yezintso zerat ngeTIANGEN Magnetic Tissue / Cell / Blood Iyonke iRNA Kit kwiTIANGEN TGuide S32 okanye iThermo Kingfisher Flex96 ngokwahlukeneyo. I-5 μl ye-200 μl eluate yafakwa kwi-1% ye-agarose gel electrophoresis, 6 V / cm. Umakishi: TIANGEN D15000 DNA Isiphawuli.
Isiphelo: Amathishu aMagnethi / iSeli / iGazi lilonke iRNA Kit inesivuno esihle sokukhupha kunye nococeko kwii-extractor ezizihambelayo.

Q: Ikholamu yokuvalela

I-A-1 Cell lysis okanye i-homogenization ayonelanga

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampuli esetyenzisiweyo okanye ukwandisa inani le-lysis buffer.

Q: Isivuno seRNA esisezantsi

A-1 I-lysis yeseli engonelanga okanye i-homogenization

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

- Nceda ubhekise kubungakanani bokusebenza.

I-A-3 RNA ayikhutshiwe ngokupheleleyo kwikholamu

--Ngasemva kokongeza amanzi asimahla e-RNase, yishiye imizuzu embalwa ngaphambi kokufaka isenti.

I-A-4 Ethanol elufeni

- Emva kokucoca, icentrifuge kwakhona kwaye ususe isikhuseli sokuhlamba kangangoko.

I-A-5 yesiko lenkcubeko yeseli ayisuswanga ngokupheleleyo

- Xa uqokelela iiseli, nceda uqiniseke ukuba ususa indawo yenkcubeko kangangoko kunokwenzeka.

A-6 Iiseli ezigcinwe kwiRNAstore azisebenzi kakuhle kwisenti

--Uxinano lweRNAstore lukhulu kunophakathi ophakathi kwenkcubeko yeseli; ke amandla e-centrifugal kufuneka anyuswe. Kuyacetyiswa kwi-centrifuge kwi-3000x g.

Umxholo we-A-7 Low RNA kunye nobuninzi kwisampulu

--Sebenzisa isampulu elungileyo ukumisela ukuba ngaba isivuno esisezantsi sibangelwa sisampulu.

Q: Ukuthotywa kweRNA

A-1 Imathiriyeli ayisiyonto intsha

Izicubu ezintsha kufuneka zigcinwe kwi-nitrojeni engamanzi kwangoko okanye kwangoko ibekwe kwi-RNAstore reagent ukuqinisekisa ukusebenza kokukhutshwa.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-3 RNase ukungcolan

- Nangona i-buffer ebonelelwe kwikhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngexesha lenkqubo yokukhupha kwaye kufuneka iphathwe ngononophelo.

Ungcoliseko lwe-4-Electrophoresis

---- Faka endaweni ye-electrophoresis buffer kwaye uqiniseke ukuba izinto ezisetyenziswayo kunye nokuLayisha iBuffer zikhululekile kungcoliseko lweRNase.

A-5 Ukulayisha kakhulu i-electrophoresis

---- Ukunciphisa inani lokulayisha isampula, ukulayishwa kwequla ngalinye akufuneki kudlule kwi-2 μg.

Umbuzo: Ungcoliseko lwe-DNA

A-1 Isixa sesampulu sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-2 Ezinye iisampulu zinomxholo ophezulu we-DNA kwaye zinokunyangwa nge-DNase.

- Yenza unyango lwe-RNase-Simahla ye-DNase kwisisombululo esifunyenweyo se-RNA, kwaye i-RNA inokusetyenziselwa ngokuthe ngqo kuvavanyo olulandelayo emva konyango, okanye inokucocwa ngakumbi ziikhithi zokucocwa zeRNA.

Q: Uyisusa njani i-RNase kwizinto ezisetyenziswayo zovavanyo nakwiglasi?

Ngeiglasi, kubhakwe kwi-150 ° C nge-4 h. Kwizikhongozeli zeplastiki, zibhaptizwe kwi-0.5 M NaOH kangange-10 min, emva koko zahlanjwa kakuhle ngamanzi angenasimahla e-RNase kwaye emva koko zibulale ukuze ususe i-RNase ngokupheleleyo. Izinto ezisetyenziswayo okanye isisombululo esisetyenzisiweyo kulingo, ngakumbi amanzi, kufuneka simahla kwi-RNase. Sebenzisa amanzi asimahla e-RNase kuwo onke amalungiselelo e-reagent (yongeza amanzi kwibhotile yeglasi ecocekileyo, yongeza i-DEPC kuxinzelelo lokugqibela lwe-0.1% (V / V), gungqisa ubusuku kunye ne-autoclave).

Bhala umyalezo wakho apha uze uyithumele kuthi