IRNAstore Reagent

Reagent engabambisiyo yokukhusela ukuthembeka kwesampulu ye-RNA.

I-RNAstore Reagent lulwelo olungasebenziyo. Ingena ngokukhawuleza kwiiseli zethishu kwaye ikhusele iiseli ezingafriziwe kwi-RNA in situ ngokuthintela ngokufanelekileyo umsebenzi we-RNase, ukuyenza ilungele ngakumbi ukuhlalutya ukubonakaliswa kweprofayili yemizimba.
Ukugcinwa kwesampulu yethishu, izicwili zinokuntywiliselwa ngokukhawuleza kwiRNAstore yokugcina ukuthintela ukuthotywa kweRNA, ukuze isampulu kungafuneki ukuba iqhutyelwe kwangoko okanye ifriziwe kwi-nitrogen engamanzi.
I-RNAstore Reagent inokusetyenziswa ngokubanzi kwiisampulu ezahlukeneyo zomqolo, kubandakanya ubuchopho, intliziyo, izintso, udakada, isibindi, imiphunga kunye ne-thymus.

Ikati. Hayi Ubungakanani bokuPakisha
4992727 100 ml

Iinkcukacha Product

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

■ Iimeko zokugcina: Le khithi ingagcinwa iveki enye kubushushu begumbi, usuku olu-1 kuma-37 ℃, kwaye kwinyanga enye ubuncinci ngo-4 ℃. Kwiisampulu zeethishu, cwilisa ngo-4 ℃ ubusuku bonke, kwaye udlulisele kwi -20 ℃ okanye -80 ℃ yokugcina ixesha elide.
■ Ukuphinda ukumisa nokubandisa: Iithishu ezibandiswe ngo -20 okanye -80 ℃ zinokubandiswa ngumkhenkce kangangezihlandlo ezingama-20 ngaphandle kokuchaphazela umgangatho wokukhutshwa kweRNA.
■ Izicelo ezisezantsi: Emva kokususa isampulu kwiRNAstore Reagent, iyonke iRNA inokukhutshwa yiTIANGEN's TRNzol, RNAprep Pure, RNAsimple reagents and kits.

Amanqaku abalulekileyo

R I-RNAstore ilungele kuphela iisampulu zenyama entsha.
■ I-RNAstore ayizilungelanga iisampulu zezicubu zezityalo.
The Umyinge wemali yeesampulu zeethishu kunye neRNAstore Reagent kufuneka ubuncinci i-1: 10 (umz. Kwi-100 mg yethishu, ubuncinci be-1 ml yeRNAstore iyafuneka).
The Ukutyeba kwecala ngalinye lesampulu akufuneki kudlule kwi-0.5 cm yokuqinisekisa ukuba iRNAstore inokungena ngokukhawuleza kwizicwili.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)


  • Egqithileyo
  • Okulandelayo:

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    Experimental Example Isixhobo: I-15 mg yeethishu zesibindi
    Indlela: I-0.5 g yeethishu zesibindi (zigcinwe kwi-RNAstore Reagent) zigcinwe kwi-37 ℃, kubushushu begumbi nakwi-4 ℃ ngokwahlukeneyo. I-RNA iyonke evela kwi-15 mg yeempuku zesampulu zesibindi ezigcinwe kumaqondo obushushu ahlukeneyo zabekelwa bucala kusetyenziswa i-TRNzol Reagent (Ikati. 4992730).
    Iziphumo: Nceda ubone lo mfanekiso ungasentla we-agarose gel electrophoresis.
    I-2-4 μl ye-100 μl eluates ilayishwe ngomgaqo ngamnye.
    C (ulawulo olululo) isampulu yethishu egcinwe ngqo kwi -80 ℃.
    I-electrophoresis yenziwa nge-6 V / cm kwimizuzu engama-30 kwi-1% ye-agarose.
    Experimental Example Isixhobo: I-15 mg yeethishu zesibindi
    Indlela: I-0.5 g yeethishu zesibindi (ezigcinwe kwi-RNAstore Reagent) zagqitywa zangama-5, 10, 15 kunye namaxesha angama-20 ngokwahlukeneyo. I-RNA iyonke evela kwi-15 mg rat rat yeesampuli zeeshubhu zesibindi ezi-freeze-thawed ngamaxesha ahlukeneyo zahlukaniswa zisebenzisa i-TRNzol Reagent (Ikati. 4992730).
    Iziphumo: Nceda ubone lo mfanekiso ungasentla we-agarose gel electrophoresis. I-2-4 μl ye-100 μl eluates ilayishwe ngomgaqo ngamnye.
    C (ulawulo olululo) isampulu yethishu egcinwe ngqo kwi -80 ℃.
    5, 10, 15, 20: amaxesha abanda ngumkhenkce weesampulu.
    I-electrophoresis yenziwa nge-6 V / cm kwimizuzu engama-30 kwi-1% ye-agarose.
    Bhala umyalezo wakho apha uze uyithumele kuthi