■ Iimeko zokugcina: Le khithi ingagcinwa iveki enye kubushushu begumbi, usuku olu-1 kuma-37 ℃, kwaye kwinyanga enye ubuncinci ngo-4 ℃. Kwiisampulu zeethishu, cwilisa ngo-4 ℃ ubusuku bonke, kwaye udlulisele kwi -20 ℃ okanye -80 ℃ yokugcina ixesha elide.
■ Ukuphinda ukumisa nokubandisa: Iithishu ezibandiswe ngo -20 okanye -80 ℃ zinokubandiswa ngumkhenkce kangangezihlandlo ezingama-20 ngaphandle kokuchaphazela umgangatho wokukhutshwa kweRNA.
■ Izicelo ezisezantsi: Emva kokususa isampulu kwiRNAstore Reagent, iyonke iRNA inokukhutshwa yiTIANGEN's TRNzol, RNAprep Pure, RNAsimple reagents and kits.
R I-RNAstore ilungele kuphela iisampulu zenyama entsha.
■ I-RNAstore ayizilungelanga iisampulu zezicubu zezityalo.
The Umyinge wemali yeesampulu zeethishu kunye neRNAstore Reagent kufuneka ubuncinci i-1: 10 (umz. Kwi-100 mg yethishu, ubuncinci be-1 ml yeRNAstore iyafuneka).
The Ukutyeba kwecala ngalinye lesampulu akufuneki kudlule kwi-0.5 cm yokuqinisekisa ukuba iRNAstore inokungena ngokukhawuleza kwizicwili.
Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)
Isixhobo: I-15 mg yeethishu zesibindi Indlela: I-0.5 g yeethishu zesibindi (zigcinwe kwi-RNAstore Reagent) zigcinwe kwi-37 ℃, kubushushu begumbi nakwi-4 ℃ ngokwahlukeneyo. I-RNA iyonke evela kwi-15 mg yeempuku zesampulu zesibindi ezigcinwe kumaqondo obushushu ahlukeneyo zabekelwa bucala kusetyenziswa i-TRNzol Reagent (Ikati. 4992730). Iziphumo: Nceda ubone lo mfanekiso ungasentla we-agarose gel electrophoresis. I-2-4 μl ye-100 μl eluates ilayishwe ngomgaqo ngamnye. C (ulawulo olululo) isampulu yethishu egcinwe ngqo kwi -80 ℃. I-electrophoresis yenziwa nge-6 V / cm kwimizuzu engama-30 kwi-1% ye-agarose. |
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Isixhobo: I-15 mg yeethishu zesibindi Indlela: I-0.5 g yeethishu zesibindi (ezigcinwe kwi-RNAstore Reagent) zagqitywa zangama-5, 10, 15 kunye namaxesha angama-20 ngokwahlukeneyo. I-RNA iyonke evela kwi-15 mg rat rat yeesampuli zeeshubhu zesibindi ezi-freeze-thawed ngamaxesha ahlukeneyo zahlukaniswa zisebenzisa i-TRNzol Reagent (Ikati. 4992730). Iziphumo: Nceda ubone lo mfanekiso ungasentla we-agarose gel electrophoresis. I-2-4 μl ye-100 μl eluates ilayishwe ngomgaqo ngamnye. C (ulawulo olululo) isampulu yethishu egcinwe ngqo kwi -80 ℃. 5, 10, 15, 20: amaxesha abanda ngumkhenkce weesampulu. I-electrophoresis yenziwa nge-6 V / cm kwimizuzu engama-30 kwi-1% ye-agarose. |
Oko yasekwa, umzi-mveliso wethu uphuhlisa iimveliso zodidi lokuqala lwehlabathi ngokubambelela kumgaqo
esemgangathweni kuqala. Iimveliso zethu ziye zaziwa ngokugqwesileyo kushishino kunye nexabiso lokuthenjwa phakathi kwabathengi abatsha nabadala ..