Isixhobo esikhulu seDNA yeGenomic

Ilungele ukucocwa kwe-DNA kwiipolysaccharides kunye nezityalo ezinobutyebi be-polyphenolics.

I-Super Plant Genomic DNA Kit yamkela ikholamu efanelekileyo ye-adsorption ejikelezayo ye-DNA kunye nenkqubo ekhethekileyo ye-buffer, enokwahlula nokucoca i-DNA esemgangathweni ye-genomic kwizicwili ezahlukeneyo zezityalo. Isisombululo esikhethekileyo semvula sinokuthi sithintele kwaye sisuse iprotein, polysaccharide, phenol kunye nokunye ukungcola kwiipolysaccharides kunye neesampulu zezityalo ezityebileyo ze-polyphenolics. I-genomic ye-DNA ekhutshiweyo inobunyulu obuphezulu, umgangatho ozinzileyo nonokuthenjwa.

Ikati. Hayi	Ubungakanani bokuPakisha
4992879	50 preps

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FAQ

Iimpawu zemveliso

Iimbonakalo

■ Ilula kwaye iyakhawuleza: I-Ultra-pure gDNA inokufunyanwa kwisithuba seyure enye.
■ Ukusetyenziswa okubanzi: Kufanelekile kwizicubu zezityalo ezahlukeneyo, ngakumbi iipolysaccharides kunye nezityalo ezinepolyphenolics.
■ Ukungabinetyhefu: Ukusebenza okukhuselekileyo kuba akukho mfuneko yephenol okanye ikloroform yokukhupha.
■ Ukucoceka okuphezulu nokusebenza ngokukuko: I-DNA emsulwa inokufumaneka ngokufanelekileyo, enokusetyenziswa ngokuthe ngqo kuvavanyo lwebhayoloji yemolekyuli enje nge-chip hybridization, NGS, njl.

Izicelo

Ifanelekile imisebenzi eyahlukeneyo yesiqhelo, kubandakanya ukuthintela ukwetyisa i-enzyme, i-PCR, ukwakhiwa kwethala leencwadi, i-chip hybridization kunye ne-Southern blot.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-Hi-DNAsecurity Kit

Okulandelayo: Hi-Swab ID Kit

I-DNA yathatyathwa kumagqabi e-100 mg Malus spectabilis kunye nomthi wepesika oneSuper Plant Genomic Kit kunye neekiti ezifanelekileyo zomthengisi A no-Q ngokwemiyalelo. M: Isiphawuli seTIANGEN D15000. I-3 μl ye-100 μl eluate yayilayishwa ngomgaqo ngamnye. Iziphumo zovavanyo zibonisa ukuba iSuper Plant Genomic Kit inesivuno esikhulu seDNA.

I-DNA yathatyathwa kumagqabi e-100 mg yezicubu zezityalo ezahlukeneyo ezineSuper Plant Genomic Kit ngokomyalelo. M: Isiphawuli seTIANGEN D15000. I-3 μl ye-100 μl eluate yayilayishwa ngomgaqo ngamnye. Iziphumo zovavanyo zibonisa ukuba iSuper Plant Genomic Kit inokusetyenziselwa ukukhupha iintlobo ngeentlobo zeesampulu zezityalo, kubandakanya iipolysaccharides kunye neesampulu zezityalo ezityebileyo ezinepolyphenolics, ngesivuno esiphakamileyo kunye nococeko.

Umbuzo: Incinci okanye ayikho i-DNA kwi-eluent.

A-1 Ukuxinana kweeseli okanye intsholongwane kwisampulu yokuqala -Tyebisa uxinzelelo lweeseli okanye iintsholongwane.

A-2 I-lysis engonelanga yeesampulu -Iisampulu azange zixutywe kakuhle kunye ne-lysis buffer. Kucetyiswa ukuba udibanise ngokucofa i-vortexing kangangezihlandlo ezi-1-2. -I-lysis yeseli engonelanga ebangelwe kukuncipha kwemisebenzi ye-proteinase K. Kucetyiswa ukuba usike izicwili zibe ziingceba kwaye wandise ixesha lokuhlamba ukususa yonke intsalela yelysate.

A-3 Ukungoneliseki kwe-DNA adsorption. -Ayikho i-ethanol okanye ipesenti esezantsi endaweni ye-100% ye-ethanol eyongezwe ngaphambi kokuba kudluliselwe i-lysate kwikholamu yokujikeleza.

A-4 Ixabiso le-pH ye-elution buffer liphantsi kakhulu. Lungisa i-pH iye phakathi kwe-8.0-8.3.

Q: I-DNA ayisebenzi kakuhle kulingelo lokuphendula kwe-enzymatic reaction.

Intsalela ye-ethanol kwi-eluent.

-Kukho intsalela yokuhlambela i-PW kwisikali. I-ethanol ingasuswa ngokufaka phakathi kwikholamu ejikelezayo ye-3-5 min, kwaye emva koko ubeke kubushushu begumbi okanye kwi-50 ℃ incubator ye-1-2 min.

Q: Ukonakaliswa kweDNA

A-1 Isampulu ayisiyonto intsha. -Khupha isampulu yeDNA elungileyo njengokulawula ukumisela ukuba ingaba iDNA ekwisampulu ihlaziyiwe.

Unyango lwangaphambi kokunyanga okungalunganga. -Yenziwe kukugaya i-nitrogen engamanzi ngokugqithisileyo, ukubuyisa ukufuma, okanye isixa esikhulu sesampulu.

Umbuzo: Uyenza njani inyanzelo yokukhutshwa kwe-gDNA?

Ukufakwa kwangaphambili kufuneka kwahluka kwiisampulu ezahlukeneyo. Kwiisampulu zezityalo, qiniseka ukuba usile kakuhle kwi-nitrogen engamanzi. Kwiisampulu zezilwanyana, homogenate okanye ugaye kakuhle kwi-nitrogen engamanzi. Kwiisampulu ezineendonga zeseli ekunzima ukuziqhekeza, ezinjenge-G + bacteria kunye nemvubelo, kucetyiswa ukuba kusetyenziswe i-lysozyme, i-lyticase okanye iindlela zoomatshini zokwaphula iindonga zeseli.

Q: Nguwuphi umahluko phakathi kweekiti ezintathu zokukhupha i-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 ye-Genomic DNA Kit isebenzisa indlela esekwe kwikholamu efuna ikloroform yokukhupha. Ifanelekile ngakumbi kwiisampulu ezahlukeneyo zezityalo, kunye nomgubo owomileyo wezityalo. I-Hi-DNAsecure Plant Kit nayo isekwe kwikholamu, kodwa kungekho mfuneko yokukhutshwa kwe-phenol / chloroform, ukuyenza ikhuseleke kwaye ingabinetyhefu. Kufanelekile ukuba izityalo ezine-polysaccharides ephezulu kunye nomxholo we-polyphenol. 4992709/4992710 DNAquick Plant System yamkela indlela esekwe kulwelo. Ukukhutshwa kwePhenol / chloroform akufuneki nako. Inkqubo yokuhlanjululwa ilula kwaye iyakhawuleza ngaphandle komda kwiisampulu zezixa zokuqala, ke abasebenzisi banokuhlengahlengisa isixa ngokuhambelana neemfuno zovavanyo. Ubungakanani obukhulu bamaqhekeza e-gDNA anokufunyanwa ngesivuno esikhulu.

Sesiphi isivuno esiqikelelweyo se-gDNA kwi-1 ml yesampulu yegazi yi-TIANamp Blood DNA Kit?

I-genomic DNA yakhutshwa kwimilinganiselo eyahlukeneyo yeesampulu zabantu zegazi yi-TIANamp Blood DNA Kit. Iziphumo zilandelayo. Iziphumo zidweliswe njengereferensi kuphela, ezona ziphumo zokukhupha zixhomekeke kwimeko yeesampulu.

Q: Ngaba i-4992207/4992208 kunye ne-4992722/4992723 ingasetyenziselwa ukukhupha i-DNA yegazi?

Ukukhutshelwa kwegazi le-DNA kungena kusetyenziswa ii-reagents ezibonelelwe kwezi khithi zimbini ngokutshintsha nje umthetho olandelwayo kumyalelo othile wokukhutshwa kwegazi le-DNA. Ikopi ethambileyo yenkqubo yokutsalwa kwegazi kwi-DNA inokukhutshwa xa uceliwe.

Q: Xa ufaka isicelo se-TIANamp Genomic DNA Kit, ungaqhekeza njani izicwili ezitsha ekumisweni kweseli?

Misa isampulu entsha nge-1 ml ye-PBS, isaline eqhelekileyo okanye i-TE buffer. Ngokupheleleyo homogenize isampuli yi homogenizer kwaye uqokelele isitshisi ukuya ezantsi kumbhobho nge-centrifuging. Yilahle into engaphaya kwamandla, kwaye uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde wenziwe khona nge-200 μl buffer GA. Ukucocwa kwe-DNA elandelayo kunokwenziwa ngokomyalelo.

Q: Uyikhetha njani imveliso yokukhutshwa kwe-DNA kwiplasma, i-serum kunye neesampulu zamanzi omzimba?

Ukucoca i-gDNA kwi-plasma, i-serum kunye neesampuli zomzimba, kuyacetyiswa i-TIANamp Micro DNA Kit. Ukucoca intsholongwane gDNA kwiisampulu ze-serum / plasma, I-TIANamp Virus DNA / RNA Kit iyacetyiswa. Ukucoca i-gDNA yebhaktiriya kwiisampulu ze-serum kunye neplasma, i-TIANamp Bacteria DNA Kit iyacetyiswa (i-lysozyme kufuneka ibandakanywe kubhaktiriya). Kwiisampulu zamathe, iHi-Swab DNA Kit kunye ne-TIANamp Bacteria DNA Kit ziyacetyiswa.

Q: Ungazikhetha njani iikiti ze-gDNA zokukhupha kwiisampulu zomngundo?

I-DNAsecure Plant Kit okanye i-DNAquick Plant System iyacetyiswa ukuba isuswe kwi-fungal genome extraction. Ukukhutshwa kwegwele le-genome, i-TIANamp Yeast DNA Kit iyacetyiswa (i-lyticase kufuneka izilungiselele).

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