■ Ukucaciswa okuqinileyo: Isikhuseli sinendlela eyodwa yokulinganisa yeK+ kunye ne-NH4+kunye ne-H-Bond yentsusa. Inkqubo yokuphendula iphuculwe kwaye inezinto ezithile eziphezulu zokunyusa umgangatho.
■ Ubuntununtunu obuphezulu: Uyakwazi ukufumana ubungakanani bokubonisa inqanaba lokubonisa elinentaphane yobuninzi.
■ Ukuphinda-phinda okuhle: Iziphumo zovavanyo zizinzile kwaye zinokuphindeka kakuhle.
■ ilula kwaye iyakhawuleza: Yitemplate kuphela, i-primer kunye ne-ddH2Kufuneka udityaniswe kummiselo wokwenza i-PCR yangempela.
■ Ukulungiswa kwe-ROX: Idayi ye-ROX ehlanganiswe ngokwahlukeneyo iyabhetyebhetye ukuyisebenzisa kwaye inokuqinisekisa iziphumo ezichanekileyo.
■ Ukusebenza ngokubanzi: Kusebenza ngokubanzi kuvavanyo olufana nohlalutyo lwembonakalo yemfuza kunye nokuchongwa kwe-asidi ye-nucleic ngendlela ye-SYBR Green kwizixhobo ezahlukeneyo zexesha le-PCR ezinje ngeABI, Stratagene, Roche, Bio-Rad kunye ne-Eppendorf.
Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)
Inkqubo ye-enzyme iqinisekisa ukukhulisa okuzinzileyo nokusebenzayo Kwezinye iinkqubo zokuphendula, xa kuthelekiswa nenkqubo yokunyusa i-enzyme, inkqubo ye-enzyme ene-othomathikhi inexabiso elomeleleyo le-fluorescence kunye nokusebenza ngokunyusa okuphezulu. |
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Thelekisa i-Dual HotStart enzyme inkqubo yolawulo oluzenzekelayo kunye nenkqubo ye-enzyme yendabuko eyodwa Kuvavanyo lokukhulisa okunzima ukukhulisa itemplate, inkqubo ye-H-bond factor buffer yeSuperReal PreMix Plus inamandla okhethekileyo okuphendula kunye nokuphindaphinda okungcono kunenkqubo yesiqhelo yesixhobo. |
Oko yasekwa, umzi-mveliso wethu uphuhlisa iimveliso zodidi lokuqala lwehlabathi ngokubambelela kumgaqo
esemgangathweni kuqala. Iimveliso zethu ziye zaziwa ngokugqwesileyo kushishino kunye nexabiso lokuthenjwa phakathi kwabathengi abatsha nabadala ..