I-TGuide yegazi ye-DNA Kit

Ukukhupha i-genomic DNA kwigazi lomntu okanye lempilo yonke.

I-TGuide yegazi ye-Genomic DNA kit yenzelwe ngokukodwa ukukhupha i-DNA (kubandakanya i-genomic DNA, i-mitochondrial DNA kunye ne-DNA ye-virus) kwigazi elipheleleyo, i-serum, iplasma kunye nee-leukocytes zisebenzisa i-TGuide Series Automated Nucleic Acid Extractor. Izinto zokuhlaziya eziyimfuneko kwi-lysis yeseli kunye nokuthotywa kweeproteni, ubuhlalu bemagnethi ngokuthe ngqo bokuthengisa i-DNA, ukuhlamba i-buffer, njl.njl. I-DNA ehlanjululweyo iya kufakwa kwitampu enetyuwa encinci. Ubude be-genomic DNA ehlanjululwe yikhithi yi-20- 30 kb, efanelekileyo kwi-PCR okanye ezinye iimpendulo ze-enzymatic.

Ikati. Hayi	Ubungakanani bokuPakisha
I-OSR-M102	Ama-preps angama-48
I-OSR-M102-T1	Ama-preps angama-48
I-OSR-M104	Ama-preps angama-48
I-OSR-M104-T1	Ama-preps angama-48

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo 1

Umzekelo wovavanyo 2

Umzekelo wovavanyo 3

FAQ

Iimpawu zemveliso

Izicelo

I-genome ehlanjululweyo ingasetyenziswa ngokuthe ngqo kwi-PCR, i-RT-PCR, i-enzyme digestion reaction, i-Southern hybridization kunye nolunye uvavanyo.

Iimbonakalo

■ Ukukhutshwa okulula nokukhawulezayo: Iimveliso ezincedisayo ze-TGuide zisekwe kumgaqo wokucocwa kwe-acid ye-asidi ngamaso emagnethi, kwaye ukukhutshwa kwe-genomic ye-DNA kungagqitywa kwimizuzu engama-44.
■ Iziphumo ezinokuthenjwa: I-genomic DNA ekhutshwe yikhithi ayinabumdaka obufana ne-RNA neprotein, kwaye inokusetyenziselwa ngqo iPCR okanye i-fluorescence quantitative PCR.
No Akukho phenol kunye nekloroform yokukhutshwa: Akukho zinyibilikisi eziyingozi emzimbeni womntu njenge phenol kunye ne chloroform ezifunekayo.
■ Ubungakanani besampula yokuqala eguqukayo: i-200 μl, i-400 μl, i-1.2 ml yegazi elipheleleyo okanye i-1-3 ml ye-leukocyte yangaphambi kokuba yahlulwe inokukhutshwa ngokuthe ngqo ngokukhetha ikhithi ehambelana nayo.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-TIANSeq rRNA yokuSebenzisa iKhithi (H / M / R)

Okulandelayo: I-TGuide Plasma DNA Ukukhutshwa kweKhithi (1.2ml)

	Iziphumo ze-genomic DNA ekhutshwe kwi-200 μl yegazi elipheleleyo liphathwe nge-anticoagulants ezahlukeneyo
	I-DNA yanyibilika kwi-200 μl elution buffer. M: 1 kb ileli 1-5 Ukufunyanwa kwe-inhibitors yee-anticoagulants ezahlukeneyo. Uhlobo ekujoliswe kulo: Uhlobo lwe-Cbl-b, 1.7 kb P: Ulawulo olufanelekileyo

	Iziphumo ze-genomic DNA ekhutshwe kwi-400 μl okanye kwi-600 μl yegazi elipheleleyo
	I-DNA yachithwa kwi-100 μl elution buffer IGenomic DNA ekhutshwe kwi-400 μl okanye kwi-600 μl yegazi elipheleleyo M: Ummakishi we-DL15000 Ukulayisha ivolumu: 2 μl

	Iziphumo ze-genomic DNA ekhutshwe kwi-1.2 ml yegazi elipheleleyo
	I-DNA yachithwa kwi-300 μl elution buffer IGenomic DNA ikhutshwe kwi-1.2 ml yegazi elipheleleyo M: Ummakishi we-DL15000; Ukulayisha ivolumu: 2 μl

Umbuzo: Incinci okanye ayikho i-DNA kwi-eluent.

A-1 Ukuxinana kweeseli okanye intsholongwane kwisampulu yokuqala -Tyebisa uxinzelelo lweeseli okanye iintsholongwane.

A-2 I-lysis engonelanga yeesampulu -Iisampulu azange zixutywe kakuhle kunye ne-lysis buffer. Kucetyiswa ukuba udibanise ngokucofa i-vortexing kangangezihlandlo ezi-1-2. -I-lysis yeseli engonelanga ebangelwe kukuncipha kwemisebenzi ye-proteinase K. Kucetyiswa ukuba usike izicwili zibe ziingceba kwaye wandise ixesha lokuhlamba ukususa yonke intsalela yelysate.

A-3 Ukungoneliseki kwe-DNA adsorption. -Ayikho i-ethanol okanye ipesenti esezantsi endaweni ye-100% ye-ethanol eyongezwe ngaphambi kokuba kudluliselwe i-lysate kwikholamu yokujikeleza.

A-4 Ixabiso le-pH ye-elution buffer liphantsi kakhulu. Lungisa i-pH iye phakathi kwe-8.0-8.3.

Q: I-DNA ayisebenzi kakuhle kulingelo lokuphendula kwe-enzymatic reaction.

Intsalela ye-ethanol kwi-eluent.

-Kukho intsalela yokuhlambela i-PW kwisikali. I-ethanol ingasuswa ngokufaka phakathi kwikholamu ejikelezayo ye-3-5 min, kwaye emva koko ubeke kubushushu begumbi okanye kwi-50 ℃ incubator ye-1-2 min.

Q: Ukonakaliswa kweDNA

A-1 Isampulu ayisiyonto intsha. -Khupha isampulu yeDNA elungileyo njengokulawula ukumisela ukuba ingaba iDNA ekwisampulu ihlaziyiwe.

Unyango lwangaphambi kokunyanga okungalunganga. -Yenziwe kukugaya i-nitrogen engamanzi ngokugqithisileyo, ukubuyisa ukufuma, okanye isixa esikhulu sesampulu.

Umbuzo: Uyenza njani inyanzelo yokukhutshwa kwe-gDNA?

Ukufakwa kwangaphambili kufuneka kwahluka kwiisampulu ezahlukeneyo. Kwiisampulu zezityalo, qiniseka ukuba usile kakuhle kwi-nitrogen engamanzi. Kwiisampulu zezilwanyana, homogenate okanye ugaye kakuhle kwi-nitrogen engamanzi. Kwiisampulu ezineendonga zeseli ekunzima ukuziqhekeza, ezinjenge-G + bacteria kunye nemvubelo, kucetyiswa ukuba kusetyenziswe i-lysozyme, i-lyticase okanye iindlela zoomatshini zokwaphula iindonga zeseli.

Q: Nguwuphi umahluko phakathi kweekiti ezintathu zokukhupha i-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 ye-Genomic DNA Kit isebenzisa indlela esekwe kwikholamu efuna ikloroform yokukhupha. Ifanelekile ngakumbi kwiisampulu ezahlukeneyo zezityalo, kunye nomgubo owomileyo wezityalo. I-Hi-DNAsecure Plant Kit nayo isekwe kwikholamu, kodwa kungekho mfuneko yokukhutshwa kwe-phenol / chloroform, ukuyenza ikhuseleke kwaye ingabinetyhefu. Kufanelekile ukuba izityalo ezine-polysaccharides ephezulu kunye nomxholo we-polyphenol. 4992709/4992710 DNAquick Plant System yamkela indlela esekwe kulwelo. Ukukhutshwa kwePhenol / chloroform akufuneki nako. Inkqubo yokuhlanjululwa ilula kwaye iyakhawuleza ngaphandle komda kwiisampulu zezixa zokuqala, ke abasebenzisi banokuhlengahlengisa isixa ngokuhambelana neemfuno zovavanyo. Ubungakanani obukhulu bamaqhekeza e-gDNA anokufunyanwa ngesivuno esikhulu.

Sesiphi isivuno esiqikelelweyo se-gDNA kwi-1 ml yesampulu yegazi yi-TIANamp Blood DNA Kit?

I-genomic DNA yakhutshwa kwimilinganiselo eyahlukeneyo yeesampulu zabantu zegazi yi-TIANamp Blood DNA Kit. Iziphumo zilandelayo. Iziphumo zidweliswe njengereferensi kuphela, ezona ziphumo zokukhupha zixhomekeke kwimeko yeesampulu.

Q: Ngaba i-4992207/4992208 kunye ne-4992722/4992723 ingasetyenziselwa ukukhupha i-DNA yegazi?

Ukukhutshelwa kwegazi le-DNA kungena kusetyenziswa ii-reagents ezibonelelwe kwezi khithi zimbini ngokutshintsha nje umthetho olandelwayo kumyalelo othile wokukhutshwa kwegazi le-DNA. Ikopi ethambileyo yenkqubo yokutsalwa kwegazi kwi-DNA inokukhutshwa xa uceliwe.

Q: Xa ufaka isicelo se-TIANamp Genomic DNA Kit, ungaqhekeza njani izicwili ezitsha ekumisweni kweseli?

Misa isampulu entsha nge-1 ml ye-PBS, isaline eqhelekileyo okanye i-TE buffer. Ngokupheleleyo homogenize isampuli yi homogenizer kwaye uqokelele isitshisi ukuya ezantsi kumbhobho nge-centrifuging. Yilahle into engaphaya kwamandla, kwaye uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde wenziwe khona nge-200 μl buffer GA. Ukucocwa kwe-DNA elandelayo kunokwenziwa ngokomyalelo.

Q: Uyikhetha njani imveliso yokukhutshwa kwe-DNA kwiplasma, i-serum kunye neesampulu zamanzi omzimba?

Ukucoca i-gDNA kwi-plasma, i-serum kunye neesampuli zomzimba, kuyacetyiswa i-TIANamp Micro DNA Kit. Ukucoca intsholongwane gDNA kwiisampulu ze-serum / plasma, I-TIANamp Virus DNA / RNA Kit iyacetyiswa. Ukucoca i-gDNA yebhaktiriya kwiisampulu ze-serum kunye neplasma, i-TIANamp Bacteria DNA Kit iyacetyiswa (i-lysozyme kufuneka ibandakanywe kubhaktiriya). Kwiisampulu zamathe, iHi-Swab DNA Kit kunye ne-TIANamp Bacteria DNA Kit ziyacetyiswa.

Q: Ungazikhetha njani iikiti ze-gDNA zokukhupha kwiisampulu zomngundo?

I-DNAsecure Plant Kit okanye i-DNAquick Plant System iyacetyiswa ukuba isuswe kwi-fungal genome extraction. Ukukhutshwa kwegwele le-genome, i-TIANamp Yeast DNA Kit iyacetyiswa (i-lyticase kufuneka izilungiselele).

Iselfowuni eDna yokuSusa iKhithi