I-TGuide I-FFPE ye-DNA yeNyathelo elinye leNyathelo

Isinyathelo esinye sokukhupha i-genome DNA kwiisampulu zeFFPE.

I-TGuide i-FFPE ye-DNA yeNyathelo elinye yeNyathelo yenzelwe ngokukodwa ukukhupha i-genome DNA kwiisampulu zeparafini zeethishu nge-TGuide automated nucleic acid extractors. Le khithi yamkela inyathelo elinye lokufudumeza indlela, ukuze i-deparaffinization kunye ne-lysis yethishu inokwenziwa ngaxeshanye, kwaye izinto ezinobungozi ezinje nge-xylene azikho. Le khithi ineendlela ezimbini zokukhupha: Kwisixa esincinci seesampuli, khetha ii-2 iiyure zelisisi; Kwisixa esikhulu seesampulu, khetha iiyure ezili-16 ngobusuku obunye. Ikhithi yamkela iselulosi efakwe kwimagneti yobuchwephesha bokukhipha ngokufanelekileyo i-genome DNA.

Ikati. Hayi	Ubungakanani bokuPakisha
I-OSR-M405	48 preps

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FAQ

Iimpawu zemveliso

Izicelo

Ukuhlanjululwa kwe-DNA kunokusetyenziswa ngokuthe ngqo kwimisebenzi eqhelekileyo yesiqhelo, kubandakanya ukwetyisa kwe-enzymatic, iPCR, ukwakhiwa kwethala leencwadi, iblot eseMazantsi nolunye uvavanyo.

Iimbonakalo

■ Ukususwa kwezinto kumatshini: Ukucocwa kunye nokunyusa amanyathelo kunokugqitywa ngokuzenzekelayo ngokubeka nje iisampulu ezinganyangekiyo ezifakwe kwiparafini kwi-cartridge ye-reagent kwaye emva koko wongeze i-Sula Oil kunye neProteinase K.
■ Iziphumo ezinokuthenjwa: I-genome DNA efumanekayo isimahla kwi-RNA kunye nongcoliseko lweprotein, kwaye inokusetyenziselwa ngqo i-PCR okanye i-PCR ye-fluorescence.
■ Ukhuselekile kwaye awunabungozi: Izinto ezifunxa izinto eziphilayo eziyingozi emzimbeni womntu njenge phenol chloroform azifuneki.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo Iiseli ze-TGuide / iTissue Genomic DNA Kit

Okulandelayo: I-TGuide yeBacteria ye-Genomic DNA Kit

Iziphumo zibonise ukuba kwiisampulu ezinkulu zevolumu, ukuveliswa kokuveliswa kwezi ndlela zimbini bekuthelekiswa; Iisampulu ezinomthamo ophakathi kunye nomncinci, imveliso yenyathelo elinye yendlela yokuhambisa (inyusa nge-TGuide) yayinkulu kunendlela yesiqhelo yokuthambisa. Ke ngoko, inyathelo elinye lokwenza into ngokuhambisa nje kuphela ukwenza lula ukusebenza, kunciphisa umngcipheko, kodwa kuphucula nokusebenza kokukhupha.
Phawula:
Isampulu yevolumu enkulu: indawo yecandelo iphakathi kwe-2.5-4 cm² kwaye ubukhulu buphakathi kwe-5-20 μm.
Isampulu yevolumu ephakathi: indawo yokuhlulwa phakathi kwe-1.5-2.5 cm² kwaye ubukhulu buphakathi kwe-5-20 μm.
Isampulu yevolumu encinci: indawo yecandelo ingaphakathi kwe-0.2-1.5 cm² kwaye ubukhulu buphakathi kwe-5-20 μm.

Umbuzo: Incinci okanye ayikho i-DNA kwi-eluent.

A-1 Ukuxinana kweeseli okanye intsholongwane kwisampulu yokuqala -Tyebisa uxinzelelo lweeseli okanye iintsholongwane.

A-2 I-lysis engonelanga yeesampulu -Iisampulu azange zixutywe kakuhle kunye ne-lysis buffer. Kucetyiswa ukuba udibanise ngokucofa i-vortexing kangangezihlandlo ezi-1-2. -I-lysis yeseli engonelanga ebangelwe kukuncipha kwemisebenzi ye-proteinase K. Kucetyiswa ukuba usike izicwili zibe ziingceba kwaye wandise ixesha lokuhlamba ukususa yonke intsalela yelysate.

A-3 Ukungoneliseki kwe-DNA adsorption. -Ayikho i-ethanol okanye ipesenti esezantsi endaweni ye-100% ye-ethanol eyongezwe ngaphambi kokuba kudluliselwe i-lysate kwikholamu yokujikeleza.

A-4 Ixabiso le-pH ye-elution buffer liphantsi kakhulu. Lungisa i-pH iye phakathi kwe-8.0-8.3.

Q: I-DNA ayisebenzi kakuhle kulingelo lokuphendula kwe-enzymatic reaction.

Intsalela ye-ethanol kwi-eluent.

-Kukho intsalela yokuhlambela i-PW kwisikali. I-ethanol ingasuswa ngokufaka phakathi kwikholamu ejikelezayo ye-3-5 min, kwaye emva koko ubeke kubushushu begumbi okanye kwi-50 ℃ incubator ye-1-2 min.

Q: Ukonakaliswa kweDNA

A-1 Isampulu ayisiyonto intsha. -Khupha isampulu yeDNA elungileyo njengokulawula ukumisela ukuba ingaba iDNA ekwisampulu ihlaziyiwe.

Unyango lwangaphambi kokunyanga okungalunganga. -Yenziwe kukugaya i-nitrogen engamanzi ngokugqithisileyo, ukubuyisa ukufuma, okanye isixa esikhulu sesampulu.

Umbuzo: Uyenza njani inyanzelo yokukhutshwa kwe-gDNA?

Ukufakwa kwangaphambili kufuneka kwahluka kwiisampulu ezahlukeneyo. Kwiisampulu zezityalo, qiniseka ukuba usile kakuhle kwi-nitrogen engamanzi. Kwiisampulu zezilwanyana, homogenate okanye ugaye kakuhle kwi-nitrogen engamanzi. Kwiisampulu ezineendonga zeseli ekunzima ukuziqhekeza, ezinjenge-G + bacteria kunye nemvubelo, kucetyiswa ukuba kusetyenziswe i-lysozyme, i-lyticase okanye iindlela zoomatshini zokwaphula iindonga zeseli.

Q: Nguwuphi umahluko phakathi kweekiti ezintathu zokukhupha i-gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

I-4992201/4992202 ye-Genomic DNA Kit isebenzisa indlela esekwe kwikholamu efuna ikloroform yokukhupha. Ifanelekile ngakumbi kwiisampulu ezahlukeneyo zezityalo, kunye nomgubo owomileyo wezityalo. I-Hi-DNAsecure Plant Kit nayo isekwe kwikholamu, kodwa kungekho mfuneko yokukhutshwa kwe-phenol / chloroform, ukuyenza ikhuseleke kwaye ingabinetyhefu. Kufanelekile ukuba izityalo ezine-polysaccharides ephezulu kunye nomxholo we-polyphenol. 4992709/4992710 DNAquick Plant System yamkela indlela esekwe kulwelo. Ukukhutshwa kwePhenol / chloroform akufuneki nako. Inkqubo yokuhlanjululwa ilula kwaye iyakhawuleza ngaphandle komda kwiisampulu zezixa zokuqala, ke abasebenzisi banokuhlengahlengisa isixa ngokuhambelana neemfuno zovavanyo. Ubungakanani obukhulu bamaqhekeza e-gDNA anokufunyanwa ngesivuno esikhulu.

Sesiphi isivuno esiqikelelweyo se-gDNA kwi-1 ml yesampulu yegazi yi-TIANamp Blood DNA Kit?

I-genomic DNA yakhutshwa kwimilinganiselo eyahlukeneyo yeesampulu zabantu zegazi yi-TIANamp Blood DNA Kit. Iziphumo zilandelayo. Iziphumo zidweliswe njengereferensi kuphela, ezona ziphumo zokukhupha zixhomekeke kwimeko yeesampulu.

Q: Ngaba i-4992207/4992208 kunye ne-4992722/4992723 ingasetyenziselwa ukukhupha i-DNA yegazi?

Ukukhutshelwa kwegazi le-DNA kungena kusetyenziswa ii-reagents ezibonelelwe kwezi khithi zimbini ngokutshintsha nje umthetho olandelwayo kumyalelo othile wokukhutshwa kwegazi le-DNA. Ikopi ethambileyo yenkqubo yokutsalwa kwegazi kwi-DNA inokukhutshwa xa uceliwe.

Q: Xa ufaka isicelo se-TIANamp Genomic DNA Kit, ungaqhekeza njani izicwili ezitsha ekumisweni kweseli?

Misa isampulu entsha nge-1 ml ye-PBS, isaline eqhelekileyo okanye i-TE buffer. Ngokupheleleyo homogenize isampuli yi homogenizer kwaye uqokelele isitshisi ukuya ezantsi kumbhobho nge-centrifuging. Yilahle into engaphaya kwamandla, kwaye uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde uphinde wenziwe khona nge-200 μl buffer GA. Ukucocwa kwe-DNA elandelayo kunokwenziwa ngokomyalelo.

Q: Uyikhetha njani imveliso yokukhutshwa kwe-DNA kwiplasma, i-serum kunye neesampulu zamanzi omzimba?

Ukucoca i-gDNA kwi-plasma, i-serum kunye neesampuli zomzimba, kuyacetyiswa i-TIANamp Micro DNA Kit. Ukucoca intsholongwane gDNA kwiisampulu ze-serum / plasma, I-TIANamp Virus DNA / RNA Kit iyacetyiswa. Ukucoca i-gDNA yebhaktiriya kwiisampulu ze-serum kunye neplasma, i-TIANamp Bacteria DNA Kit iyacetyiswa (i-lysozyme kufuneka ibandakanywe kubhaktiriya). Kwiisampulu zamathe, iHi-Swab DNA Kit kunye ne-TIANamp Bacteria DNA Kit ziyacetyiswa.

Q: Ungazikhetha njani iikiti ze-gDNA zokukhupha kwiisampulu zomngundo?

I-DNAsecure Plant Kit okanye i-DNAquick Plant System iyacetyiswa ukuba isuswe kwi-fungal genome extraction. Ukukhutshwa kwegwele le-genome, i-TIANamp Yeast DNA Kit iyacetyiswa (i-lyticase kufuneka izilungiselele).

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