I-TRNzol yendalo iphela

Ifomula entsha yokuphuculwa kwesampulu ebanzi yokuziqhelanisa.

I-TRNzol Universal Reagent inokusetyenziselwa ukwahlula i-RNA iyonke kwiisampulu ezinjengentsholongwane, ibhaktiriya, ukungunda, isilwanyana, izihlunu zezityalo kunye nolwelo lomzimba ngobuchule obungcono be-lysis kunye nemvakalelo ephezulu. Igcina ingqibelelo ye-RNA ngelixa iphazamisa iiseli kunye nokunyibilikisa izinto zeseli ngexesha lesampula homogenization. I-RNA ebekelwe bucala yile mveliso inokusebenza ngokuthe ngqo njengeetemplate zokufumana ukuhla okanye ezinye iinkqubo.

Ikati. Hayi	Ubungakanani bokuPakisha
4992730	100 ml

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

FAQ

Iimpawu zemveliso

Iimbonakalo

■ Ukuguquguquka okuphezulu: Uluhlu lwasendle lokuqalisa ivolumu yesampulu, efanelekileyo kwisampulu enkulu yokukhupha isampulu kwimpendulo enye.
■ Isivuno esikhulu: Indlela yokuthambisa yandisa imveliso yeRNA kwisampulu.
■ Ukusetyenziswa okubanzi: Ilungele iisampulu ezininzi ezahlukeneyo ezifana nezicubu zezityalo nezilwanyana, iiseli ezikhulisiweyo, igazi, ulwelo lomzimba, njl.
■ Ukusebenza okukhawulezileyo: IGenomic DNA inokufumaneka kwisithuba seyure enye.

Ukucaciswa

Uhlobo: Kwemvula esekelwe
Isampuli: Intsholongwane, ibhaktiriya, ukungunda, isilwanyana, izicwili zezityalo, iiseli ezinenkcubeko kunye nolwelo lomzimba.
Ithagethi: RNA
Ixesha lokusebenza: ~ 1 iyure
Izicelo: I-TRNzol I-reagent yendalo iphela inciphisa ukungcola okunje nge-DNA kunye neeproteni kwi-RNA ecociweyo, kwaye inokusetyenziselwa ngokuthe ngqo kuvavanyo lwebhayoloji ezahlukeneyo ezinjengeNorthern Blot, iDot Blot, ukuvavanywa kwePolyA, ukuguqulelwa kwe-vitro, Uhlalutyo lokhuselo lweRNase, ukwakhiwa kwethala leencwadi le-cDNA , I-RT-PCR, ixesha lokwenene le-PCR kunye nokulandelelana okuphezulu.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo RNAprep Pure Micro Kit

Okulandelayo: RNAprep Isisele esinyulu / iBacteria Kit

Workflow

Indlela: 30 mg yeethishu zesibindi, i-100 mg yamagqabi erayisi aqokelelwa ngokugaya i-nitrogen engamanzi; 1 × 10⁶Iiseli ezikhulisiweyo ze-HepG2 kunye ne-700 μl Saccharomyces Cerevisiae medium medium (OD600 = 0.9) zaqokelelwa nge-centrifugation. I-1 ml ye-TRNzol Universal Reagent evela kwi-TIANGEN kunye neemveliso ezifanelekileyo ezivela kumthengisi u-L no-T zongezwa kwi-aliquot nganye yesampulu kwaye ukukhutshwa kwe-RNA kwenziwa emva kwemigaqo ebonelelwe ngumthengisi ngamnye. Umthamo we-elution yi-80 μl, 50 μl, 30 μl kunye ne-30 μl kwiisampulu ezine ngokwahlukeneyo. I-3 μl ye-eluate yayilayishwa kwindlela nganye.
I-MIII: I-TIANGEN Ummakishi III;
I-electrophoresis yenziwa nge-6 V / cm kwimizuzu engama-30 kwi-1% ye-agarose.
Iziphumo: I-TIANGEN TRNzol Universal Reagent inokukhupha ubunyulu obuphezulu kunye nokuthembeka kwe-RNA kwisibindi sebuzi, amagqabi erayisi, iiseli ezinamasiko kunye neesampulu zegwele, ngokusebenza kakuhle. Umgangatho weRNA uthelekiswa okanye uphakame kancinci kunowomthengisi weL kunye neemveliso zeT.

Q: Ikholamu yokuvalela

I-A-1 Cell lysis okanye i-homogenization ayonelanga

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampuli esetyenzisiweyo okanye ukwandisa inani le-lysis buffer.

Q: Isivuno seRNA esisezantsi

A-1 I-lysis yeseli engonelanga okanye i-homogenization

Ukunciphisa ukusetyenziswa kwesampula, ukwandisa inani le-buffer ye-lysis, ukwandisa i-homogenization kunye nexesha le-lysis.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

- Nceda ubhekise kubungakanani bokusebenza.

I-A-3 RNA ayikhutshiwe ngokupheleleyo kwikholamu

--Ngasemva kokongeza amanzi asimahla e-RNase, yishiye imizuzu embalwa ngaphambi kokufaka isenti.

I-A-4 Ethanol elufeni

- Emva kokucoca, icentrifuge kwakhona kwaye ususe isikhuseli sokuhlamba kangangoko.

I-A-5 yesiko lenkcubeko yeseli ayisuswanga ngokupheleleyo

- Xa uqokelela iiseli, nceda uqiniseke ukuba ususa indawo yenkcubeko kangangoko kunokwenzeka.

A-6 Iiseli ezigcinwe kwiRNAstore azisebenzi kakuhle kwisenti

--Uxinano lweRNAstore lukhulu kunophakathi ophakathi kwenkcubeko yeseli; ke amandla e-centrifugal kufuneka anyuswe. Kuyacetyiswa kwi-centrifuge kwi-3000x g.

Umxholo we-A-7 Low RNA kunye nobuninzi kwisampulu

--Sebenzisa isampulu elungileyo ukumisela ukuba ngaba isivuno esisezantsi sibangelwa sisampulu.

Q: Ukuthotywa kweRNA

A-1 Imathiriyeli ayisiyonto intsha

Izicubu ezintsha kufuneka zigcinwe kwi-nitrojeni engamanzi kwangoko okanye kwangoko ibekwe kwi-RNAstore reagent ukuqinisekisa ukusebenza kokukhutshwa.

Isixa sesampulu esingu-A-2 sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-3 RNase ukungcolan

- Nangona i-buffer ebonelelwe kwikhithi ingenayo i-RNase, kulula ukungcolisa i-RNase ngexesha lenkqubo yokukhupha kwaye kufuneka iphathwe ngononophelo.

Ungcoliseko lwe-4-Electrophoresis

---- Faka endaweni ye-electrophoresis buffer kwaye uqiniseke ukuba izinto ezisetyenziswayo kunye nokuLayisha iBuffer zikhululekile kungcoliseko lweRNase.

A-5 Ukulayisha kakhulu i-electrophoresis

---- Ukunciphisa inani lokulayisha isampula, ukulayishwa kwequla ngalinye akufuneki kudlule kwi-2 μg.

Umbuzo: Ungcoliseko lwe-DNA

A-1 Isixa sesampulu sikhulu kakhulu

---- Ukunciphisa inani lesampula.

A-2 Ezinye iisampulu zinomxholo ophezulu we-DNA kwaye zinokunyangwa nge-DNase.

- Yenza unyango lwe-RNase-Simahla ye-DNase kwisisombululo esifunyenweyo se-RNA, kwaye i-RNA inokusetyenziselwa ngokuthe ngqo kuvavanyo olulandelayo emva konyango, okanye inokucocwa ngakumbi ziikhithi zokucocwa zeRNA.

Q: Uyisusa njani i-RNase kwizinto ezisetyenziswayo zovavanyo nakwiglasi?

Ngeiglasi, kubhakwe kwi-150 ° C nge-4 h. Kwizikhongozeli zeplastiki, zibhaptizwe kwi-0.5 M NaOH kangange-10 min, emva koko zahlanjwa kakuhle ngamanzi angenasimahla e-RNase kwaye emva koko zibulale ukuze ususe i-RNase ngokupheleleyo. Izinto ezisetyenziswayo okanye isisombululo esisetyenzisiweyo kulingo, ngakumbi amanzi, kufuneka simahla kwi-RNase. Sebenzisa amanzi asimahla e-RNase kuwo onke amalungiselelo e-reagent (yongeza amanzi kwibhotile yeglasi ecocekileyo, yongeza i-DEPC kuxinzelelo lokugqibela lwe-0.1% (V / V), gungqisa ubusuku kunye ne-autoclave).

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