DNA Bisulfite Conversion Kit Featured Image

Kit yokuguqula i-Bisulfite Kit

Ukuguqulwa nokucocwa kunokugqitywa ngeyure e-2, ngenqanaba lokuguqula ukuya kuthi ga kwi-99%.

I-DNA Bisulfite Conversion Kit yenzelwe ngokukodwa ukuguqulwa kwe-DNA bisulfite kuphando lwe-DNA methylation. Unyango lweesampulu ze-DNA kusetyenziswa le khithi inokugqitywa ngeyure ezi-2, kwaye inqanaba lokuguqulwa kwe-cytosine engafakwanga i-uracil ingaphezulu kwama-99%. Le khithi yamkela icandelo elahlukileyo le-DNA ekhusela iarhente, eqinisekisa umgangatho kunye nokubuyiselwa kweDNA emva kokuguqulwa. Ukongeza, le khithi ikwasebenzisa indlela kwikholamu yokususwa kweqela le-sulphurous acid group, eyenza ukuba yonke inkqubo isebenze lula.

Ikati. Hayi	Ubungakanani bokuPakisha
4992447	50 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

■ ilula kwaye iyakhawuleza: Inkqubo yokuguqula nokucoca inokugqitywa kwiiyure ezi-2, kunye neemfuno eziphantsi zezixhobo kunye nezixhobo, ezifanelekileyo kumaziko ophando kuwo onke amanqanaba.
■ Izinga lokuguqulwa okuphezulu: Izinga lokuguqulwa kwe-cytosine engafakwanga imethylated kwi-uracil kwisampulu ye-DNA ingaphezulu kwama-99%.
Sensitivity Ubuntununtunu obuphezulu: Le khithi inokuqhubekekisa iisampulu zeDNA ezisezantsi njenge-500 pg ukuya kuthi ga kwi-2.5 μg.

Ukucaciswa

Uhlobo: Ukuguqulwa kweBisulfite
Isampulu yeDNA: 500 pg-2.5 μg
Ixabiso lokuguqulwa: > 99%
Ixesha lokusebenza: ~ 2 iyure
Izicelo: Iisampulu ze-DNA ezenziwa yile khithi zilungele i-methylation ethile ye-PCR / qPCR, ukulandelelana kunye ne-microarray.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo I-TIANSeq mRNA yokuBamba iKhithi

Okulandelayo: Ikhithi yeMethylation-specipc PCR (MSP)

Itheyibhile 1: Izinga lokuguqulwa kwe-C engafakwanga u “C” ukuya ku “U”
Ukusebenzisa i-genome yomntu njengezinto zokulinga, izinga lokubuyisela isampuli linokufikelela kuma-65%, kwaye umlinganiselo we "C" ongagqithwanga ku "U" unokufikelela ngaphezu kwama-99%.

Table 1: Conversion rate of unmethylated “C” to “U” Using human genome as experimental material, the recovery rate of the sample can reach 65%, and the ratio of unmethylated “C” to “U” can reach more than 99%.