I-Mini Eplasmid Kit II engapheliyo

Ukucocwa kwebakala le-plasmid DNA engenasiphelo.

I-EndoFree Mini Plasmid Kit II yamkela itekhnoloji ye-silica membrane adsorption ngokufanelekileyo nangokukhethekileyo ibopha i-plasmid DNA. Inkqubo ekhethekileyo yokususa i-endotoxin ingasusa ngokufanelekileyo ukungcola okunje nge-endotoxin kunye neeproteni. Inkqubo yonke yokukhupha ithatha iyure enye, efanelekileyo kwaye ekhawulezayo. Isivuno sokukhupha i-plasmid sinxulumene nenombolo yeplasmid, uhlobo lwebacteria kunye neemeko zenkcubeko. Ukuya kuthi ga kwi-70 μg ye-plasmid DNA inokukhutshwa kwi-5-15 ml yeebacteria zenkcubeko yobusuku bonke.

Ikati. Hayi	Ubungakanani bokuPakisha
4992422	50 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

Yield Isivuno esikhawulezayo nesiphezulu: 5-70 μg plasmid DNA inokutsalwa kwiyure enye.
■ Ukucoceka okuphezulu: Inkqubo ekhethekileyo yokususa i-endotoxin edityaniswe ne-Spin Column CP4 inokukhangisa ngokuthe ngqo i-nucleic acid kwaye isuse i-endotoxin iye kwinqanaba elincinci.
■ Uluhlu olubanzi lwezicelo: Zilungele imifuniselo yesiqhelo efana nokuthintela ukwetyisa ukutya, i-PCR, ukulandelelana, ukugoba, ukuguqula, kunye nokuvavanywa okuphezulu njengokunyanga kwemfuza, iseli ye-microinjection, ukuthulisa imfuza, ukukhuphela, njl.

Ukuveliswa kwePlasmid

Plasmid Yield

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo Ikhithi ye-EndoFree Maxi Plasmid

Okulandelayo: I-TIANprep N96 Magnetic Plasmid Kit

Ukulungele ukukhutshwa kweeplasmids eziphezulu / eziphantsi
Umzobo 1. Ukukhutshwa kwe-DNA yeplasmid ukusuka kumthamo ohlukeneyo webacteria yenkcubeko kusetyenziswa i-EndoFree Mini Plasmid Kit II.
Umthamo we-Elution: 200 μl; Ukulayisha ivolumu: 3 μl ikopi ephantsi yeplasmid pBR322, 2 μl ikopi ephezulu yeplasmid pBS.
I-electrophoresis yenziwa nge-6 V / cm kwimizuzu engama-30 kwi-1% ye-agarose.
Ukuhanjiswa kweseli yiplasmid ecocekileyo

Umzobo 2 I-PEGFP plasmid ehlanjululwe yi-EndoFree Mini Plasmid Kit II yahanjiswa kwiiseli ezingama-293T. Inkcazo ye-GFP yafunyanwa kwi-48 h emva kokuhanjiswa

Bhala umyalezo wakho apha uze uyithumele kuthi