I-TIANprep Rapid N96 Plasmid Kit

Ukuhamba okuphezulu, ukukhutshwa ngokukhawuleza kwenani elincinci leplasmid.

Ikhithi yamkela isopropanol indlela yemvula yokufumana iplasmid DNA. Ikhithi yamkela ipleyiti yokucoca kakuhle eyi-96 kunye ne-Buffer III, ephuhliswe ngokukodwa yi-TIANGEN, kwaye inokukhupha ngokukhawuleza nangokucocekileyo iplasmid DNA. Kufanelekile ukulungiswa ngokukhawuleza kweplasmids. I-1.3 ml yekopi ephezulu yeplasmid yenkcubeko inokusetyenzwa kakuhle, kwaye iisampulu ezingama-96 zeDNA yeplasmid ikhutshwa ngokukhawuleza. Esona sivuno sikhulu equleni li-10-20 μg (isivuno sinxulumene nezinto ezinjengeenombolo zeplasmid, imeko yebhaktiriya, njl. I-Plasmid DNA ekhutshwe yikhithi inokusetyenziswa kuvavanyo lwebhayoloji njengokuthintela ukwetyisa i-enzyme, ukulandelelana, ukuvavanywa kwethala leencwadi, ukubopha kunye notshintsho.

Ikati. Hayi:	Ubungakanani bokuPakisha
4992868	4 preps
4992869	24 preps

Thumela i-imeyile kuthi

Iinkcukacha Product

Ukuhamba komsebenzi

Umzekelo wovavanyo

Iimpawu zemveliso

Iimbonakalo

■ Ilula kwaye ilula: Iisampulu ezingama-96 zinokutsalwa kwiiyure ezingama-2-3.
■ Exhotywe nge-reagent ekhethekileyo ye-TIANRed, inokuchonga ngokucacileyo inqanaba le-lysis yesampulu kwinkqubo yokukhupha ukuqinisekisa i-plasmid DNA ephezulu kakhulu.
■ Ukucoceka okuphezulu: Iplasmid yokusulungeka okuphezulu inokusetyenziswa ngokuthe ngqo kumalinge asezantsi.

Zonke iimveliso zingenziwa ngokwezifiso kwi-ODM / OEM. Iinkcukacha,nceda ucofe iNkonzo eChongiweyo (i-ODM / i-OEM)

Egqithileyo Ikhithi ye-EndoFree Midi Plasmid

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TIANprep Rapid N96 Plasmid Kit01

	I-Plasmid DNA yatsalwa kwi-1 ml yobusuku obukhulisiweyo be-Escherichia coli (TOP10) (LB medium) isebenzisa i-TIANprep Rapid N96 Plasmid Kit, kunye ne-avareji ye-8-10 μg yokucoceka okuphezulu kwe-pBS plasmid yafunyanwa. I-µl plasmid ilayishwe equleni ngalinye le-1% ye-agarose gel, kwaye yafunyanwa yi-electrophoresis.
	I-Plasmid DNA yatsalwa kwi-1 ml yobusuku obukhulisiweyo be-Escherichia coli (TOP10) (LB medium) isebenzisa i-TIANprep Rapid N96 Plasmid Kit, kunye ne-avareji ye-8-10 μg yokucoceka okuphezulu kwe-pBS plasmid yafunyanwa. Vector: i-pBS plasmid; Ubunzima: TOP10. I-plasmid yalandelwa yi-ABI. I-PRISM 3730XL ye-DNA sequencer